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UniProtKB/Swiss-Prot entry Q9VWH4


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDH3A_DROME
Primary accession number Q9VWH4
Secondary accession numbers Q8IQW9 Q8SXH8
Integrated into Swiss-Prot on April 13, 2004
Sequence was last modified on May 1, 2000 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 54)
Name and origin of the protein
Protein name Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor]
Synonyms EC 1.1.1.41
Isocitric dehydrogenase
NAD(+)-specific ICDH
Gene name
Name: l(1)G0156
ORFNames: CG12233
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[2]
GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE014298; AAF48965.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAN09496.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY089629; AAL90367.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_573388.1; -.
NP_728257.1; -.
UniGene Dm.222
3D structure databases
HSSP P08200; 1ISO. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
ModBase Q9VWH4.
Protein-protein interaction databases
IntAct Q9VWH4; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-002607-MON; -.
Organism-specific databases
FlyBase FBgn0027291; l(1)G0156.
Gene expression databases
ArrayExpress Q9VWH4; -.
GermOnline CG12233; Drosophila melanogaster.
Ontologies
GO
GO:0005811; Cellular component: lipid particle (inferred from direct assay from FlyBase).
GO:0005739; Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0004449; Molecular function: isocitrate dehydrogenase (NAD+) activity (inferred from sequence or structural similarity from UniProtKB).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006099; Biological process: tricarboxylic acid cycle (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00175; mito_nad_idh; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS Q9VWH4.
ProtoNet Q9VWH4.
Genome annotation databases
Ensembl CG12233; Drosophila melanogaster. [Contig view]
GeneID 32940; -.
KEGG dme:Dmel_CG12233; -.
NMPDR fig|7227.3.peg.18632; -.
Phylogenomic databases
HOGENOM Q9VWH4; -.
Other
NextBio 781145; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Complete proteome; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   377        Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial. PRO_0000014442
METAL   249   249        Magnesium or manganese (By similarity). 
METAL   273   273        Magnesium or manganese (By similarity). 
METAL   277   277        Magnesium or manganese (By similarity). 
BINDING   131   131        Substrate (By similarity). 
BINDING   141   141        Substrate (By similarity). 
BINDING   162   162        Substrate (By similarity). 
BINDING   249   249        Substrate (By similarity). 
SITE   169   169  1     Critical for catalysis (By similarity). 
SITE   216   216  1     Critical for catalysis (By similarity). 
VAR_SEQ   1    32        MAARFIQKILNQLGLIAARDAPAVTATPAVSQ -> MAARFIQKI (in isoform A). VSP_050698
Sequence information
Length: 377 AA [This is the length of the unprocessed precursor] Molecular weight: 40844 Da [This is the MW of the unprocessed precursor] CRC64: FB3F30905B8939B3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAARFIQKIL NQLGLIAARD APAVTATPAV SQVNATPAAS RSYSSGTKKV TLIPGDGIGP 

        70         80         90        100        110        120 
EISAAVQKIF TAANVPIEWE AVDVTPVRGP DGKFGIPQAA IDSVNTNKIG LKGPLMTPVG 

       130        140        150        160        170        180 
KGHRSLNLAL RKEFNLYANV RPCRSLEGYK TLYDDVDVVT IRENTEGEYS GIEHEIVDGV 

       190        200        210        220        230        240 
VQSIKLITEE ASKRVAEYAF QYAKNNNRKK VTVVHKANIM RMSDGLFLRC VRDMAQKFPE 

       250        260        270        280        290        300 
IQFEEKYLDT VCLNMVQNPG KYDVLVMPNL YGDILSDMCA GLVGGLGLTP SGNMGLNGAL 

       310        320        330        340        350        360 
FESVHGTAPD IAGKDLANPT ALLLSAVMML RHMELNTYAD KIERAAFETI KEGKYLTGDL 

       370 
GGRAKCSEFT NEICAKL 

Q9VWH4 in FASTA format

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