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UniProtKB/Swiss-Prot entry Q9SLH3


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RGA_ARATH
Primary accession number Q9SLH3
Secondary accession numbers O23642 O23725 Q941D4
Integrated into Swiss-Prot on November 22, 2005
Sequence was last modified on May 1, 2000 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 41)
Name and origin of the protein
Protein name DELLA protein RGA
Synonyms Repressor on the ga1-3 mutant
GAI-related sequence
Restoration of growth on ammonia protein 1
GRAS family protein 10
AtGRAS-10
Gene name
Name: RGA
Synonyms: GRS, RGA1
OrderedLocusNames: At2g01570
ORFNames: F2I9.19
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
STRAIN=cv. Columbia;
DOI=10.1016/S0014-5793(97)00590-5; PubMed=9237632 [NCBI, ExPASy, EBI, Israel, Japan]
Truong H.-N., Caboche M., Daniel-Vedele F.;
"Sequence and characterization of two Arabidopsis thaliana cDNAs isolated by functional complementation of a yeast gln3 gdh1 mutant.";
FEBS Lett. 410:213-218(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Landsberg erecta;
PubMed=9389651 [NCBI, ExPASy, EBI, Israel, Japan]
Peng J., Carol P., Richards D.E., King K.E., Cowling R.J., Murphy G.P., Harberd N.P.;
"The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses.";
Genes Dev. 11:3194-3205(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/45471; PubMed=10617197 [NCBI, ExPASy, EBI, Israel, Japan]
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
Nature 402:761-768(1999).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[5]
FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MUTAGENESIS OF ASP-478.
DOI=10.1105/tpc.10.2.155; PubMed=9490740 [NCBI, ExPASy, EBI, Israel, Japan]
Silverstone A.L., Ciampaglio C.N., Sun T.-P.;
"The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway.";
Plant Cell 10:155-169(1998).
[6]
FUNCTION.
PubMed=11606551 [NCBI, ExPASy, EBI, Israel, Japan]
King K.E., Moritz T., Harberd N.P.;
"Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA.";
Genetics 159:767-776(2001).
[7]
FUNCTION.
PubMed=11606552 [NCBI, ExPASy, EBI, Israel, Japan]
Dill A., Sun T.-P.;
"Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana.";
Genetics 159:777-785(2001).
[8]
DOMAIN, AND MUTAGENESIS OF 44-ASP--ALA-60.
DOI=10.1073/pnas.251534098; PubMed=11717468 [NCBI, ExPASy, EBI, Israel, Japan]
Dill A., Jung H.-S., Sun T.-P.;
"The DELLA motif is essential for gibberellin-induced degradation of RGA.";
Proc. Natl. Acad. Sci. U.S.A. 98:14162-14167(2001).
[9]
TISSUE SPECIFICITY.
DOI=10.1101/gad.969002; PubMed=11877383 [NCBI, ExPASy, EBI, Israel, Japan]
Lee S., Cheng H., King K.E., Wang W., He Y., Hussain A., Lo J., Harberd N.P., Peng J.;
"Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI/RGA-like gene whose expression is up-regulated following imbibition.";
Genes Dev. 16:646-658(2002).
[10]
FUNCTION.
DOI=10.1038/nature01387; PubMed=12610625 [NCBI, ExPASy, EBI, Israel, Japan]
Fu X., Harberd N.P.;
"Auxin promotes Arabidopsis root growth by modulating gibberellin response.";
Nature 421:740-743(2003).
[11]
DEGRADATION.
DOI=10.1105/tpc.010827; PubMed=12724538 [NCBI, ExPASy, EBI, Israel, Japan]
McGinnis K.M., Thomas S.G., Soule J.D., Strader L.C., Zale J.M., Sun T.-P., Steber C.M.;
"The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase.";
Plant Cell 15:1120-1130(2003).
[12]
FUNCTION.
DOI=10.1105/tpc.015685; PubMed=14615596 [NCBI, ExPASy, EBI, Israel, Japan]
Achard P., Vriezen W.H., Van Der Straeten D., Harberd N.P.;
"Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function.";
Plant Cell 15:2816-2825(2003).
[13]
FUNCTION.
DOI=10.1242/dev.00992; PubMed=14973286 [NCBI, ExPASy, EBI, Israel, Japan]
Cheng H., Qin L., Lee S., Fu X., Richards D.E., Cao D., Luo D., Harberd N.P., Peng J.;
"Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function.";
Development 131:1055-1064(2004).
[14]
PROBABLE UBIQUITINATION, AND INTERACTION WITH GID2.
DOI=10.1105/tpc.020958; PubMed=15155881 [NCBI, ExPASy, EBI, Israel, Japan]
Dill A., Thomas S.G., Hu J., Steber C.M., Sun T.-P.;
"The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation.";
Plant Cell 16:1392-1405(2004).
[15]
INTERACTION WITH GID2, AND PHOSPHORYLATION.
DOI=10.1105/tpc.021386; PubMed=15161962 [NCBI, ExPASy, EBI, Israel, Japan]
Fu X., Richards D.E., Fleck B., Xie D., Burton N., Harberd N.P.;
"The Arabidopsis mutant sleepy1gar2-1 protein promotes plant growth by increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA protein substrates.";
Plant Cell 16:1406-1418(2004).
[16]
INTERACTION WITH GID2.
DOI=10.1104/pp.104.039578; PubMed=15173565 [NCBI, ExPASy, EBI, Israel, Japan]
Tyler L., Thomas S.G., Hu J., Dill A., Alonso J.M., Ecker J.R., Sun T.-P.;
"Della proteins and gibberellin-regulated seed germination and floral development in Arabidopsis.";
Plant Physiol. 135:1008-1019(2004).
[17]
FUNCTION.
DOI=10.1073/pnas.0402377101; PubMed=15128937 [NCBI, ExPASy, EBI, Israel, Japan]
Yu H., Ito T., Zhao Y., Peng J., Kumar P., Meyerowitz E.M.;
"Floral homeotic genes are targets of gibberellin signaling in flower development.";
Proc. Natl. Acad. Sci. U.S.A. 101:7827-7832(2004).
[18]
FUNCTION.
DOI=10.1007/s00425-005-0057-3; PubMed=16034591 [NCBI, ExPASy, EBI, Israel, Japan]
Cao D., Hussain A., Cheng H., Peng J.;
"Loss of function of four DELLA genes leads to light- and gibberellin-independent seed germination in Arabidopsis.";
Planta 223:105-113(2005).
[19]
MUTAGENESIS OF GLN-341.
DOI=10.1104/pp.104.057646; PubMed=15734906 [NCBI, ExPASy, EBI, Israel, Japan]
Muangprom A., Thomas S.-G., Sun T.-P., Osborn T.C.;
"A novel dwarfing mutation in a green revolution gene from Brassica rapa.";
Plant Physiol. 137:931-938(2005).
Comments
  • FUNCTION: Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation.
  • SUBUNIT: Interacts directly with the GID2/SLY1 component of the SCF(GID2) complex.
  • INTERACTION:
    Q9STX3:GID2; NbExp=2; IntAct=EBI-963624, EBI-619033;
  • SUBCELLULAR LOCATION: Nucleus.
  • TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in roots, rosette leaves, bolting and mature stems, young and mature siliques, flower buds and influorescences.
  • DOMAIN: The DELLA motif is required for its GA-induced degradation but not for the interaction with GID2.
  • PTM: Phosphorylated. Phosphorylation may increase the interaction with GID2.
  • PTM: Ubiquitinated. Upon GA application it is ubiquitinated by the SCF(GID2) complex, leading to its subsequent degradation.
  • SIMILARITY: Belongs to the GRAS family. DELLA subfamily.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y11336; CAA72177.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y15194; CAA75493.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC005560; AAC67333.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY052239; AAK97709.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY054160; AAL06821.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT010467; AAQ65090.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D84426; D84426.
RefSeq NP_178266.1; -.
UniGene At.24012
3D structure databases
ModBase Q9SLH3.
Protein-protein interaction databases
IntAct Q9SLH3; -.
Organism-specific databases
GeneFarm 4238; 427.
TAIR At2g01570; -.
Gene expression databases
ArrayExpress Q9SLH3; -.
GermOnline AT2G01570; Arabidopsis thaliana.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0009740; Biological process: gibberellic acid mediated signaling (inferred from electronic annotation from UniProtKB-KW).
GO:0007275; Biological process: multicellular organismal development (inferred from electronic annotation from UniProtKB-KW).
GO:0006355; Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR005202; GRAS.
Graphical view of domain structure.
Pfam PF03514; GRAS; 1.
Pfam graphical view of domain structure.
PROSITE PS50985; GRAS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9SLH3.
ProtoNet Q9SLH3.
Genome annotation databases
GeneID 814686; -.
GenomeReviews CT485783_GR; AT2G01570.
KEGG ath:AT2G01570; -.
NMPDR fig|3702.1.peg.7760; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Developmental protein; Gibberellin signaling pathway; Nucleus; Phosphoprotein; Repressor; Transcription; Transcription regulation; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   587  587     DELLA protein RGA. PRO_0000132234
MOTIF   44    48  5     DELLA motif. 
MOTIF   323   327  5     VHIID. 
MOTIF   423   427  5     LXXLL motif. 
COMPBIAS   190   200  11     Poly-Thr. 
MUTAGEN   44    60        Missing: In rga-delta17; induces resistance to GA-induced degradation but does not affect nuclear localization. 
MUTAGEN   341   341        Q->R: Causes a semidwarf phenotype by abolishing the interaction with GID2 leading to prevent its degradation. 
MUTAGEN   478   478        D->N: In rga-2; partially suppresses phenotypic defects of GA-mutant ga1-3. 
CONFLICT   86    86        T -> A (in Ref. 2; CAA75493). 
CONFLICT   139   139        K -> E (in Ref. 2; CAA75493). 
CONFLICT   203   203        G -> A (in Ref. 1 and 2). 
CONFLICT   209   209        V -> D (in Ref. 4; AAK97709). 
CONFLICT   383   383        I -> V (in Ref. 2; CAA75493). 
CONFLICT   587   587        Y -> H (in Ref. 1; CAA72177). 
Sequence information
Length: 587 AA [This is the length of the unprocessed precursor] Molecular weight: 64035 Da [This is the MW of the unprocessed precursor] CRC64: FC92E7F9408072AA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKRDHHQFQG RLSNHGTSSS SSSISKDKMM MVKKEEDGGG NMDDELLAVL GYKVRSSEMA 

        70         80         90        100        110        120 
EVALKLEQLE TMMSNVQEDG LSHLATDTVH YNPSELYSWL DNMLSELNPP PLPASSNGLD 

       130        140        150        160        170        180 
PVLPSPEICG FPASDYDLKV IPGNAIYQFP AIDSSSSSNN QNKRLKSCSS PDSMVTSTST 

       190        200        210        220        230        240 
GTQIGGVIGT TVTTTTTTTT AAGESTRSVI LVDSQENGVR LVHALMACAE AIQQNNLTLA 

       250        260        270        280        290        300 
EALVKQIGCL AVSQAGAMRK VATYFAEALA RRIYRLSPPQ NQIDHCLSDT LQMHFYETCP 

       310        320        330        340        350        360 
YLKFAHFTAN QAILEAFEGK KRVHVIDFSM NQGLQWPALM QALALREGGP PTFRLTGIGP 

       370        380        390        400        410        420 
PAPDNSDHLH EVGCKLAQLA EAIHVEFEYR GFVANSLADL DASMLELRPS DTEAVAVNSV 

       430        440        450        460        470        480 
FELHKLLGRP GGIEKVLGVV KQIKPVIFTV VEQESNHNGP VFLDRFTESL HYYSTLFDSL 

       490        500        510        520        530        540 
EGVPNSQDKV MSEVYLGKQI CNLVACEGPD RVERHETLSQ WGNRFGSSGL APAHLGSNAF 

       550        560        570        580 
KQASMLLSVF NSGQGYRVEE SNGCLMLGWH TRPLITTSAW KLSTAAY 

Q9SLH3 in FASTA format

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