ID   MDAR3_ARATH             Reviewed;         434 AA.
AC   Q9LFA3; Q8LBV9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   16-DEC-2008, entry version 48.
DE   RecName: Full=Probable monodehydroascorbate reductase, cytoplasmic isoform 3;
DE            Short=MDAR 3;
DE            EC=1.6.5.4;
GN   OrderedLocusNames=At3g52880; ORFNames=F8J2_50;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016720; PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M.,
RA   Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B.,
RA   Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P.,
RA   De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P.,
RA   Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V.,
RA   Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S.,
RA   Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G.,
RA   Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B.,
RA   Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G.,
RA   Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J.,
RA   Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D.,
RA   Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D.,
RA   Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W.,
RA   Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J.,
RA   Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P.,
RA   Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S.,
RA   Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V.,
RA   Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C.,
RA   Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E.,
RA   Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA   Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of monodehydroascorbate to
CC       ascorbate, oxidizing NADH in the process (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 monodehydroascorbate = NAD(+) + 2
CC       ascorbate.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase family.
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DR   EMBL; AL132969; CAB86892.1; -; Genomic_DNA.
DR   EMBL; AF360197; AAK25907.1; -; mRNA.
DR   EMBL; AY057576; AAL09815.1; -; mRNA.
DR   EMBL; AY045666; AAK74024.1; -; mRNA.
DR   EMBL; AY070718; AAL50062.1; -; mRNA.
DR   EMBL; AY060525; AAL31138.1; -; mRNA.
DR   EMBL; AY091403; AAM14342.1; -; mRNA.
DR   EMBL; AY125492; AAM83213.1; -; mRNA.
DR   EMBL; AY086968; AAM64531.1; -; mRNA.
DR   PIR; T47545; T47545.
DR   RefSeq; NP_190856.1; -.
DR   UniGene; At.24483; -.
DR   PRIDE; Q9LFA3; -.
DR   GeneID; 824454; -.
DR   GenomeReviews; BA000014_GR; AT3G52880.
DR   KEGG; ath:AT3G52880; -.
DR   NMPDR; fig|3702.1.peg.16560; -.
DR   TAIR; At3g52880; -.
DR   ArrayExpress; Q9LFA3; -.
DR   GermOnline; AT3G52880; Arabidopsis thaliana.
DR   GO; GO:0048046; C:apoplast; IDA:TAIR.
DR   GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR   GO; GO:0005782; C:peroxisomal matrix; IDA:TAIR.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0016656; F:monodehydroascorbate reductase (NADH) activity; IEA:EC.
DR   GO; GO:0042744; P:hydrogen peroxide catabolic process; TAS:TAIR.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0009626; P:plant-type hypersensitive response; IDA:TAIR.
DR   GO; GO:0046686; P:response to cadmium ion; IEP:TAIR.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR001100; Pyr_nuc-diS_OxRdtase.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00411; PNDRDTASEI.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; NAD; Oxidoreductase;
KW   Redox-active center.
FT   CHAIN         1    434       Probable monodehydroascorbate reductase,
FT                                cytoplasmic isoform 3.
FT                                /FTId=PRO_0000209138.
FT   CONFLICT    206    206       D -> N (in Ref. 3; AAM64531).
SQ   SEQUENCE   434 AA;  46487 MW;  DE7FFF0F349784A0 CRC64;
     MAEKSFKYII LGGGVSAGYA AKEFANQGVQ PGELAVISKE AVAPYERPAL SKGYLFPEGA
     ARLPGFHCCV GSGGEKLLPE SYKQKGIELI LSTEIVKADL SAKSLVSATG DVFKYQTLII
     ATGSTVLRLT DFGVKGADSK NILYLREIDD ADKLVEAIKA KKGGKAVVVG GGYIGLELSA
     VLRINNLDVT MVFPEPWCMP RLFTADIAAF YETYYTNKGV KIIKGTVASG FTAQPNGEVK
     EVQLKDGRTL EADIVIVGVG AKPLTSLFKG QVEEDKGGIK TDAFFKTSVP DVYAVGDVAT
     FPLKMYGDVR RVEHVDHSRK SAEQAVKAIK AAEGGAAVEE YDYLPFFYSR SFDLSWQFYG
     DNVGDSVLFG DSNPSNPKPR FGAYWVQGGK VVGAFMEGGS GDENKALAKV AKARPSAESL
     DELVKQGISF AAKI
//