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UniProtKB/Swiss-Prot entry Q9I4G8

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LOXA_PSEAE
Primary accession number Q9I4G8
Secondary accession numbers None
Integrated into Swiss-Prot on May 10, 2004
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 45)
Name and origin of the protein
Protein name Arachidonate 15-lipoxygenase [Precursor]
Synonyms 15-LOX
EC 1.13.11.33
Gene name
Name: loxA
OrderedLocusNames: PA1169
From
Pseudomonas aeruginosa [TaxID: 287] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
DOI=10.1038/35023079; PubMed=10984043 [NCBI, ExPASy, EBI, Israel, Japan]
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.";
Nature 406:959-964(2000).
[2]
 FUNCTION.
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
DOI=10.1073/pnas.0307308101; PubMed=14766977 [NCBI, ExPASy, EBI, Israel, Japan]
Vance R.E., Hong S., Gronert K., Serhan C.N., Mekalanos J.J.;
"The opportunistic pathogen Pseudomonas aeruginosa carries a secretable arachidonate 15-lipoxygenase.";
Proc. Natl. Acad. Sci. U.S.A. 101:2135-2139(2004).
Comments
  • FUNCTION: Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid (HpETE) which is rapidly reduced to hydroxyeicosatetraenoic acid (HETE). Could act on exogenous human-derived substrates to potentially modulate the local inflammatory responses during P.aeruginosa infections.
  • CATALYTIC ACTIVITY: Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.
  • COFACTOR: Binds 1 iron ion per subunit (By similarity).
  • SUBCELLULAR LOCATION: Periplasm. Secreted, extracellular space. Note=Periplasmic with some activity further secreted to the extracellular milieu.
  • SIMILARITY: Belongs to the lipoxygenase family.
  • SIMILARITY: Contains 1 lipoxygenase domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE004091; AAG04558.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A83499; A83499.
RefSeq NP_249860.1; -.
3D structure databases
HSSP P08170; 2SBL. [HSSP ENTRY / PDB]
ModBase Q9I4G8.
Enzyme and pathway databases
BioCyc PAER208964:PA1169-MON; -.
Organism-specific databases
PseudoCAP PA1169; -.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0042597; Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0050473; Molecular function: arachidonate 15-lipoxygenase activity (inferred from electronic annotation from EC).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000907; LipOase.
IPR013819; LipOase_C.
Graphical view of domain structure.
PANTHER PTHR11771; LipOase; 1.
Pfam PF00305; Lipoxygenase; 1.
Pfam graphical view of domain structure.
PRINTS PR00087; LIPOXYGENASE.
PROSITE PS00711; LIPOXYGENASE_1; 1.
PS00081; LIPOXYGENASE_2; 1.
PS51393; LIPOXYGENASE_3; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9I4G8.
ProtoNet Q9I4G8.
Genome annotation databases
GeneID 879547; -.
GenomeReviews AE004091_GR; PA1169.
KEGG pae:PA1169; -.
NMPDR fig|208964.1.peg.1170; -.
Phylogenomic databases
HOGENOM Q9I4G8; -.
Genome annotation databases
CMR Q9I4G8; PA1169.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase; Periplasm; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    19  19     Potential. 
CHAIN   20   685  666     Arachidonate 15-lipoxygenase. PRO_0000018329
DOMAIN   122   685  564     Lipoxygenase. 
METAL   377   377        Iron; catalytic (By similarity). 
METAL   382   382        Iron; catalytic (By similarity). 
METAL   555   555        Iron; catalytic (By similarity). 
METAL   559   559        Iron; catalytic (By similarity). 
METAL   685   685        Iron; via carboxylate; catalytic (By similarity). 
Sequence information
Length: 685 AA [This is the length of the unprocessed precursor] Molecular weight: 74804 Da [This is the MW of the unprocessed precursor] CRC64: B6A307595AE16A5E [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKRRSVLLSG VALSGTALAN DSIFFSPLKY LGAEQQRSID ASRSLLDNLI PPSLPQYDNL 

        70         80         90        100        110        120 
AGKLARRAVL TSKKLVYVWT ENFGNVKGVP MARSVPLGEL PNVDWLLKTA GVIVELIVNF 

       130        140        150        160        170        180 
VASLPASAAA QFERIATGLS GDLEAARQVH EALLEEAKND PAAAGSLLLR FTELQTRVIA 

       190        200        210        220        230        240 
ILTRVGLLVD DILKSASNLV TQRGQGDGLN RFRAVFGTLR LPEVADSFRD DEAFAYWRVA 

       250        260        270        280        290        300 
GPNPLLIRRV DALPANFPLG EEQFRRVMGA DDSLLEAAAS RRLYLLDYAE LGKLAPSGAV 

       310        320        330        340        350        360 
DKLLTGTGFA YAPIALFALG KDRARLLPVA IQCGQDPATH PMFVRPAESE SDLYWGWQMA 

       370        380        390        400        410        420 
KTVVQVAEEN YHEMFVHLAQ THLVSEAFCL ATQRTLAPSH PLHVLLAPHF EGTLFINEGA 

       430        440        450        460        470        480 
ARILLPSAGF IDVMFAAPIQ DTQATAGGNR LGFDFYRGML PESLKARNVD DPLALPDYPY 

       490        500        510        520        530        540 
RDDGLLVWNA IRQWAADYVA VYYASDGDVT ADVELAAWVG EVIGSGKVAG FRPITGRSQL 

       550        560        570        580        590        600 
VEVLTMVIFT ASAQHAAVNF PQPSMMTYAP AICAMSAAPA PDSPSGKSEA DWLKMMPPTL 

       610        620        630        640        650        660 
VALEKVNIYH LLGSVYHGRL GDYRQTGFPY APVFSDRRVT ASGGPLERFQ ARLKEVEATI 

       670        680 
RTRNQARRRP YEYLLPSRIP ASTNI
Q9I4G8 in FASTA format

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