[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. STRAIN=ATCC 24725 / CBS 481.73 / CCRC 36200 / NRRL 6361 / VKM-F-1767; DOI=10.1007/s002940050010; PubMed=10672447 [NCBI, ExPASy, EBI, Israel, Japan] Yadav J.S., Loper J.C.; "Cytochrome P450 oxidoreductase gene and its differentially terminated cDNAs from the white rot fungus Phanerochaete chrysosporium."; Curr. Genet. 37:65-73(2000).
[2]	KINETIC MECHANISM. DOI=10.1016/S0006-291X(02)02600-1; PubMed=12437968 [NCBI, ExPASy, EBI, Israel, Japan] Warrilow A.G.S., Lamb D.C., Kelly D.E., Kelly S.L.; "Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism."; Biochem. Biophys. Res. Commun. 299:189-195(2002).

Comments

FUNCTION: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
CATALYTIC ACTIVITY: NADPH + n oxidized hemoprotein = NADP⁺ + n reduced hemoprotein.
COFACTOR: FAD (By similarity).
COFACTOR: FMN (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity).
SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF193060; AAG31349.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF193061; AAG31350.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF193062; AAG31351.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P00388; 1AMO. [HSSP ENTRY / PDB]

ModBase

Q9HDG2.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0010181;	Molecular function: FMN binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0003958;	Molecular function: NADPH-hemoprotein reductase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR015702; NADPH_Cyt_Red.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

PANTHER

PTHR19384:SF17; NADPH_Cyt_Red; 1.

Pfam

PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00369; FLAVODOXIN.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; FAD; Flavoprotein; FMN; Membrane; NADP; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 736`	`736`	`NADPH--cytochrome P450 reductase.`	`PRO_0000167607`
`TRANSMEM`	`8 24`	`17`	`Potential.`
`DOMAIN`	`66 216`	`151`	`Flavodoxin-like.`
`DOMAIN`	`269 546`	`278`	`FAD-binding FR-type.`
`NP_BIND`	`162 193`	`32`	`FMN (By similarity).`
`NP_BIND`	`310 321`	`12`	`FAD (By similarity).`
`NP_BIND`	`452 463`	`12`	`FAD (By similarity).`
`NP_BIND`	`572 590`	`19`	`NADP (By similarity).`
`NP_BIND`	`682 698`	`17`	`NADP (By similarity).`

Sequence information

Length: 736 AA [This is the length of the unprocessed precursor]

Molecular weight: 81628 Da [This is the MW of the unprocessed precursor]

CRC64: AC45F12C96198AB1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAVSSSSDVI VLSVGIILAA LYLFREQIFS AAKPKTVQVP SSKAAAGGNG NPRDFIAKMK 

        70         80         90        100        110        120 
EGKKRIVIFY GSQTGTAEEY AIRLAKEAKS KFGLASLVCD PEEYDFENLD QVPEDCCVFF 

       130        140        150        160        170        180 
VMATYGEGEP TDNAVQLCQN LSDESFEFSN GEHKLPGLKY VIFGLGNKTY EHYNLISRNV 

       190        200        210        220        230        240 
DRDLQKMGAI RIGERGEGDD DKSMEEDYLE WKDGMWEAFA KAMNVEEGQG GDSPDFVVTE 

       250        260        270        280        290        300 
VFDHPEEKVY LGELSARALT RTKGIHDAKN PYPAPIIAAK ELFAPGSDRN CVHIELSTES 

       310        320        330        340        350        360 
SGITYQHGDH VGVWPSNADK EVDRLLYALG LHEKKDTVIN IESLDPALAK VPFPVPTTYA 

       370        380        390        400        410        420 
TVLRHYIDIS ALAGRQILGV LAKFAPNPEA EAVLKDLNSN KEHYQNIVAN GCMKLGEVLQ 

       430        440        450        460        470        480 
YAAGNDLHAD PTASNTTAWK IPFDIIVSSI PRLQPRYYSI SSSPKLYPNA IHATVVVLKY 

       490        500        510        520        530        540 
KSEKAPRVEE RWIYGVGSNF LLNLKYASHH DKAATLVSDD SPSEPSIVSH YPTYSIEGPR 

       550        560        570        580        590        600 
GAYKQGDVVK VPIHVRRSTF RLPTNPKSPV IMIGPGTGVA PFRGFVQERV AMARRTIEKH 

       610        620        630        640        650        660 
GPEGLADWGP IRLYYGCRRS DQDFLYKDEW PEYAKELHGK FIMRCAFSRE PPYKPDGSKI 

       670        680        690        700        710        720 
YVQDLIWEDA EQIADAILNG KGYVYICGDA KSMSKSVEET LCRILGEAKG GSAEVEGAAE 

       730 
LKLLKERNRL LLDVWS

Q9HDG2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools