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UniProtKB/Swiss-Prot entry Q9AXM6

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name T10H_TAXCU
Primary accession number Q9AXM6
Secondary accession numbers None
Integrated into Swiss-Prot on February 12, 2003
Sequence was last modified on June 1, 2001 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 36)
Name and origin of the protein
Protein name Taxane 10-beta-hydroxylase
Synonyms EC 1.14.13.76
5-alpha-taxadienol-10-beta-hydroxylase
Cytochrome P450 725A1
Gene name
Name: CYP725A1
From
Taxus cuspidata (Japanese yew) [TaxID: 99806] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Coniferopsida; Coniferales; Taxaceae; Taxus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
DOI=10.1073/pnas.98.4.1501; PubMed=11171980 [NCBI, ExPASy, EBI, Israel, Japan]
Schoendorf A., Rithner C.D., Williams R.M., Croteau R.B.;
"Molecular cloning of a cytochrome P450 taxane 10beta-hydroxylase cDNA from Taxus and functional expression in yeast.";
Proc. Natl. Acad. Sci. U.S.A. 98:1501-1506(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF318211; AAK00946.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
ModBase Q9AXM6.
Enzyme and pathway databases
BioCyc MetaCyc:MON-13401; -.
Ontologies
GO
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0050597; Molecular function: taxane 10-beta-hydroxylase activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0042617; Biological process: paclitaxel biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
Other
ProtoNet Q9AXM6.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Taxol biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   497  497     Taxane 10-beta-hydroxylase. PRO_0000052202
METAL   443   443        Iron (heme axial ligand) (By similarity). 
Sequence information
Length: 497 AA [This is the length of the unprocessed precursor] Molecular weight: 56691 Da [This is the MW of the unprocessed precursor] CRC64: CFE40415ABC43814 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDSFIFLRSI GTKFGQLESS PAILSLTLAP ILAIILLLLF RYNHRSSVKL PPGKLGFPLI 

        70         80         90        100        110        120 
GETIQLLRTL RSETPQKFFD DRLKKFGPVY MTSLIGHPTV VLCGPAGNKL VLSNEDKLVE 

       130        140        150        160        170        180 
MEGPKSFMKL IGEDSIVAKR GEDHRILRTA LARFLGAQAL QNYLGRMSSE IGHHFNEKWK 

       190        200        210        220        230        240 
GKDEVKVLPL VRGLIFSIAS TLFFDVNDGH QQKQLHHLLE TILVGSLSVP LDFPGTRYRK 

       250        260        270        280        290        300 
GLQARLKLDE ILSSLIKRRR RDLRSGIASD DQDLLSVLLT FRDEKGNSLT DQGILDNFSA 

       310        320        330        340        350        360 
MFHASYDTTV APMALIFKLL YSNPEYHEKV FQEQLEIIGN KKEGEEISWK DLKSMKYTWQ 

       370        380        390        400        410        420 
AVQESLRMYP PVFGIFRKAI TDIHYDGYTI PKGWRVLCSP YTTHLREEYF PEPEEFRPSR 

       430        440        450        460        470        480 
FEDEGRHVTP YTYVPFGGGL RTCPGWEFSK IEILLFVHHF VKNFSSYIPV DPNEKVLSDP 

       490 
LPPLPANGFS IKLFPRS
Q9AXM6 in FASTA format

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