NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=FVB/N;
TISSUE=Mammary tumor;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).

[2]

ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-885, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1016/j.molcel.2006.06.026; PubMed=16916647 [NCBI, ExPASy, EBI, Israel, Japan]
Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.;
"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.";
Mol. Cell 23:607-618(2006).

Comments

CATALYTIC ACTIVITY: Sarcosine + acceptor + H₂O = glycine + formaldehyde + reduced acceptor.
COFACTOR: Binds 1 FAD covalently per monomer (By similarity).
PATHWAY: Amine and polyamine degradation; sarcosine degradation; formaldehyde and glycine from sarcosine: step 1/1 .
SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
SIMILARITY: Belongs to the gcvT family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC003456; AAH03456.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_619606.1; -.

UniGene

Mm.278467

3D structure databases

ModBase

Q99LB7.

PTM databases

PhosphoSite

Q99LB7; -.

Organism-specific databases

MGI

MGI:2183102; Sardh.

Gene expression databases

ArrayExpress

Q99LB7; -.

CleanEx

MM_SARDH; -.

GermOnline

ENSMUSG00000009614; Mus musculus.

Ontologies

GO:0005759;	Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004047;	Molecular function: aminomethyltransferase activity (inferred from electronic annotation from InterPro).
GO:0008480;	Molecular function: sarcosine dehydrogenase activity (inferred from electronic annotation from EC).
GO:0006546;	Biological process: glycine catabolic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR006076; FAD-dep_OxRdtase.
IPR013977; GCV_T_C.
IPR006222; GCV_T_N.
Graphical view of domain structure.

Pfam

PF01266; DAO; 1.
PF01571; GCV_T; 1.
PF08669; GCV_T_C; 1.
Pfam graphical view of domain structure.

Genome annotation databases

Ensembl

ENSMUSG00000009614; Mus musculus. [Contig view]

GeneID

192166; -.

KEGG

mmu:192166; -.

Phylogenomic databases

HOGENOM

Q99LB7; -.

HOVERGEN

Q99LB7; -.

Other

371164; -.

Sardh; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acetylation; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Phosphoprotein; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 919`		`Sarcosine dehydrogenase, mitochondrial.`	`PRO_0000010771`
`MOD_RES`	`109 109`		`Tele-8alpha-FAD histidine (By similarity).`
`MOD_RES`	`778 778`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`885 885`		`N6-acetyllysine.`

Sequence information

Length: 919 AA [This is the length of the unprocessed precursor]

Molecular weight: 101682 Da [This is the MW of the unprocessed precursor]

CRC64: 4908ACB540C9D305 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASLSRVLRV AATCPRGRAA WNLGLQPLAT EARPTTEKSV PYQRTLKEEA QGASVVPQGP 

        70         80         90        100        110        120 
SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGAVLLERE RLTSGTTWHT AGLLWQLRPS 

       130        140        150        160        170        180 
DVEVELLAHT RQVVSRDLEE ETGLHTGWIQ NGGLFIASNQ QRLNEYKRLM SLGKAYGIES 

       190        200        210        220        230        240 
HVLSPAETKS LYPLMNVDDL YGTLYVPQDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG 

       250        260        270        280        290        300 
IRVRTDDFGV RRVAAVETEH GSIQTPCVVN CAGVWASKVG RMAGVKVPLV AMHHAYVVTE 

       310        320        330        340        350        360 
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWE EVSDKFAFGL FDLDWDVFTQ 

       370        380        390        400        410        420 
HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG 

       430        440        450        460        470        480 
CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHTRWIRER SHESYAKNYS VVFPHDEPLA 

       490        500        510        520        530        540 
GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG NQAHKDYTYS 

       550        560        570        580        590        600 
RLLGDEYTFD FPPHHHMIQK ECLACRGAAA VFNMSYFGKF YLLGVDARKA ADWLFSADVN 

       610        620        630        640        650        660 
RPPGSTVYTC MLNQRGGTES DLTVSRLAPG TQASPLVPAF EGDCYYLAVG GAVAQHNWSH 

       670        680        690        700        710        720 
INTVLQDQEF RCQLMDSSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG 

       730        740        750        760        770        780 
HLVRAIRLSF VGELGWELHV PRASCLPVYR AVMAAGARHG LVNAGYRAID SLSIEKGYRH 

       790        800        810        820        830        840 
WHADLRPDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLICLT VEEEVPMFGL 

       850        860        870        880        890        900 
EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGEYA LERMGVTYAA 

       910 
QVHLKSPFDP DNKRVKGIY

Q99LB7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools