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UniProtKB/Swiss-Prot entry Q99027


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name COMP_BACSU
Primary accession number Q99027
Secondary accession number O05226
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on May 30, 2000 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 68)
Name and origin of the protein
Protein name Sensor protein comP
Synonym EC 2.7.13.3
Gene name
Name: comP
OrderedLocusNames: BSU31690
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=BD630;
PubMed=2116363 [NCBI, ExPASy, EBI, Israel, Japan]
Weinrauch Y., Penchev R., Dubnau E., Smith I., Dubnau D.;
"A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.";
Genes Dev. 4:860-872(1990).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=9274030 [NCBI, ExPASy, EBI, Israel, Japan]
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.;
"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).";
Microbiology 143:2769-2774(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
STRAIN=168;
PubMed=1715859 [NCBI, ExPASy, EBI, Israel, Japan]
Weinrauch Y., Msadek T., Kunst F., Dubnau D.;
"Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.";
J. Bacteriol. 173:5685-5693(1991).
[5]
POLYMORPHISM IN COMX; COMQ AND COMP.
DOI=10.1128/JB.183.2.451-460.2001; PubMed=11133937 [NCBI, ExPASy, EBI, Israel, Japan]
Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D.;
"Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.";
J. Bacteriol. 183:451-460(2001).
[6]
FUNCTION.
DOI=10.1111/j.1365-2958.2005.04749.x; PubMed=16091051 [NCBI, ExPASy, EBI, Israel, Japan]
Comella N., Grossman A.D.;
"Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.";
Mol. Microbiol. 57:1159-1174(2005).
Comments
  • FUNCTION: Sensor in the two-component regulatory system comP/comA involved in a major quorum response pathway that regulates the development of genetic competence. Plays a role in sporulation, at least partly interchangeable with that of spoIIJ.
  • CATALYTIC ACTIVITY: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
  • SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
  • PTM: Autophosphorylates on a histidine and transfers the phosphate group onto an aspartate in comA, thus activating it.
  • MISCELLANEOUS: The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.
  • SIMILARITY: Contains 1 histidine kinase domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X54010; CAA37957.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z93932; CAB07903.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99120; CAB15157.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M22856; AAA22319.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M71283; AAA22324.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A35848; A35848.
B69604; B69604.
RefSeq NP_391047.1; -.
3D structure databases
ModBase Q99027.
Enzyme and pathway databases
BioCyc BSUB224308:BSU3164-MON; -.
Organism-specific databases
SubtiList BG10380; comP. [Micado]
Family and domain databases
InterPro IPR003594; ATP_bd_ATPase.
IPR011712; Sig_transdc_His_kin_sub3_dim/P.
IPR005467; Sig_transdc_His_kinase_core.
Graphical view of domain structure.
Gene3D G3DSA:3.30.565.10; ATP_bd_ATPase; 1.
Pfam PF02518; HATPase_c; 1.
PF07730; HisKA_3; 1.
Pfam graphical view of domain structure.
SMART SM00387; HATPase_c; 1.
SMART graphical view of domain structure.
PROSITE PS50109; HIS_KIN; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q99027.
Genome annotation databases
GeneID 938866; -.
GenomeReviews AL009126_GR; BSU31690.
KEGG bsu:BSU31690; -.
NMPDR fig|224308.1.peg.3172; -.
Phylogenomic databases
HOGENOM Q99027; -.
Genome annotation databases
CMR Q99027; BSU31690.
Other
ProtoNet Q99027.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Competence; Complete proteome; Kinase; Membrane; Phosphoprotein; Sporulation; Transferase; Transmembrane; Two-component regulatory system.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   769  769     Sensor protein comP. PRO_0000074736
TOPO_DOM   1     9  9     Cytoplasmic (Potential). 
TRANSMEM   10    33  24     Potential. 
TOPO_DOM   34   113  80     Extracellular (Potential). 
TRANSMEM   114   134  21     Potential. 
TOPO_DOM   135   144  10     Cytoplasmic (Potential). 
TRANSMEM   145   167  23     Potential. 
TOPO_DOM   168   235  68     Extracellular (Potential). 
TRANSMEM   236   257  22     Potential. 
TOPO_DOM   258   272  15     Cytoplasmic (Potential). 
TRANSMEM   273   295  23     Potential. 
TOPO_DOM   296   299  4     Extracellular (Potential). 
TRANSMEM   300   323  24     Potential. 
TOPO_DOM   324   337  14     Cytoplasmic (Potential). 
TRANSMEM   338   357  20     Potential. 
TOPO_DOM   358   361  4     Extracellular (Potential). 
TRANSMEM   362   383  22     Potential. 
TOPO_DOM   384   769  386     Cytoplasmic (Potential). 
DOMAIN   571   769  199     Histidine kinase. 
MOD_RES   456   456        Phosphohistidine; by autocatalysis (By similarity). 
CONFLICT   604   604        S -> C (in Ref. 1; CAA37957/AAA22319). 
CONFLICT   610   610        D -> Y (in Ref. 1; CAA37957/AAA22319). 
CONFLICT   628   628        E -> G (in Ref. 1; CAA37957/AAA22319). 
CONFLICT   636   637        QL -> PV (in Ref. 1; CAA37957/AAA22319). 
Sequence information
Length: 769 AA [This is the length of the unprocessed precursor] Molecular weight: 89317 Da [This is the MW of the unprocessed precursor] CRC64: E5CEACCE278DE5B4 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKNLIKKFTI AVIVLSILYI SYTTYISMNG IIIGTKIHKN DKSQFMIEEI SESSYGQFVG 

        70         80         90        100        110        120 
LRQADIILKI NKEKPSDKHL KWGYLSHINS LDILRSGKKI HLKDFDLVTL NRPYSFFLFV 

       130        140        150        160        170        180 
LPLFFYFLSI ICIFYILKVN KKRRSFAAYI LILLLLDISI AYISAGGPFR GHIINRYINL 

       190        200        210        220        230        240 
FTFISSPILY LQFIQRYLGE IGKTFLNRIS FLYIIPIFNL GIEFFQDYLQ VDIDFLATLN 

       250        260        270        280        290        300 
LVSFATLTLF SFSAIYLHLN KYKYAEHSFI LKLLILTNTL SFAPFLIFFV LPIIFTGNYI 

       310        320        330        340        350        360 
FPALASASLL VLIPFGLVYQ FVANKMFDIE FILGRMRYYA LLAMIPTLLI VGALVLFDVM 

       370        380        390        400        410        420 
DIQMNPVRQT VFFFVVMFAV FYFKEVMDFK FRLKRFSEKF NYQDSIFKYT QLMRGVTSLQ 

       430        440        450        460        470        480 
QVFKELKNTI LDVLLVSKAY TFEVTPDHKV IFLDKHEVGP DWNFYQEEFE NVTSEIGKII 

       490        500        510        520        530        540 
EVNQGFLMKV GERGGSSYVL LCLSNINTPR LTRDEISWLK TLSFYTSVSM ENVLHIEELM 

       550        560        570        580        590        600 
EHLKDLKQEG TNPIWLKKLM FAIEEKQRSG LARDLHDSVL QDLISLKRQC ELFLGDFKKD 

       610        620        630        640        650        660 
DNPSREEVQD KLVQMNEQMS DVISMTRETC HELRPQLLYD LGLVKALSKL VAHEQERVPF 

       670        680        690        700        710        720 
HIRLNTGRFT ASLDLDSQLN LYRIIQEFLS NAVKHSQATD VLIMLISIQN KIVLHYEDDG 

       730        740        750        760 
VGFDQEKNTE HSMSMGLSGI KERVRALDGR LRIETSEGKG FKADIEIEL 

Q99027 in FASTA format

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