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UniProtKB/Swiss-Prot entry Q96LR5


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UB2E2_HUMAN
Primary accession number Q96LR5
Secondary accession numbers None
Integrated into Swiss-Prot on May 24, 2004
Sequence was last modified on December 1, 2001 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 64)
Name and origin of the protein
Protein name Ubiquitin-conjugating enzyme E2 E2
Synonyms EC 6.3.2.19
Ubiquitin-protein ligase E2
Ubiquitin carrier protein E2
UbcH8
Gene name
Name: UBE2E2
Synonyms: UBCH8
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
PubMed=9371400 [NCBI, ExPASy, EBI, Israel, Japan]
Kimura M., Hattori T., Matsuda Y., Yoshioka T., Sumi N., Umeda Y., Nakashima S., Okano Y.;
"cDNA cloning, characterization, and chromosome mapping of UBE2E2 encoding a human ubiquitin-conjugating E2 enzyme.";
Cytogenet. Cell Genet. 78:107-111(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain;
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AK057886; BAB71605.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC022332; AAH22332.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_689866.1; -.
UniGene Hs.475688
3D structure databases
PDB
1Y6L; X-ray; 1.85 A; A/B/C=54-201.[ExPASy / RCSB / EBI]
PDBsum 1Y6L; -.
ModBase Q96LR5.
PTM databases
PhosphoSite Q96LR5; -.
Organism-specific databases
GeneCards GC03P023221; -.
H-InvDB HIX0018506; -.
HGNC HGNC:12478; UBE2E2.
GenAtlas UBE2E2.
HPA HPA003303; -.
MIM 602163; gene. [NCBI / EBI]
PharmGKB PA37128; -.
GeneCards Q96LR5.
Gene expression databases
ArrayExpress Q96LR5; -.
CleanEx HS_UBE2E2; -.
GermOnline ENSG00000182247; Homo sapiens.
Ontologies
GO
GO:0042296; Molecular function: ISG15 ligase activity (inferred from direct assay from HGNC).
GO:0004842; Molecular function: ubiquitin-protein ligase activity (inferred from electronic annotation from EC).
GO:0032020; Biological process: ISG15-protein conjugation (inferred from direct assay from HGNC).
GO:0051246; Biological process: regulation of protein metabolic process (inferred from electronic annotation from InterPro).
GO:0006511; Biological process: ubiquitin-dependent protein catabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.
Gene3D G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
PANTHER PTHR11621; UBQ-conjugat_E2; 1.
Pfam PF00179; UQ_con; 1.
Pfam graphical view of domain structure.
ProDom PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00212; UBCc; 1.
SMART graphical view of domain structure.
PROSITE PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE Q96LR5; -.
Genome annotation databases
Ensembl ENSG00000182247; Homo sapiens. [Contig view]
GeneID 7325; -.
KEGG hsa:7325; -.
Phylogenomic databases
HOGENOM Q96LR5; -.
HOVERGEN Q96LR5; -.
Other
LinkHub Q96LR5; -.
NextBio 28662; -.
SOURCE UBE2E2; Homo sapiens.
ProtoNet Q96LR5.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Ligase; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   201  201     Ubiquitin-conjugating enzyme E2 E2. PRO_0000082472
ACT_SITE   139   139        Glycyl thioester intermediate (By similarity). 
HELIX   55    69  15      
STRAND   75    82  8      
STRAND   86    92  7      
TURN   98   101  4      
STRAND   103   109  7      
TURN   112   115  4      
STRAND   120   125  6      
HELIX   141   143  3      
TURN   144   146  3      
HELIX   153   165  13      
HELIX   175   183  9      
HELIX   185   199  15      
Sequence information
Length: 201 AA [This is the length of the unprocessed precursor] Molecular weight: 22255 Da [This is the MW of the unprocessed precursor] CRC64: 3286FB51E2B9CD3E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSTEAQRVDD SPSTSGGSSD GDQRESVQQE PEREQVQPKK KEGKISSKTA AKLSTSAKRI 

        70         80         90        100        110        120 
QKELAEITLD PPPNCSAGPK GDNIYEWRST ILGPPGSVYE GGVFFLDITF SPDYPFKPPK 

       130        140        150        160        170        180 
VTFRTRIYHC NINSQGVICL DILKDNWSPA LTISKVLLSI CSLLTDCNPA DPLVGSIATQ 

       190        200 
YMTNRAEHDR MARQWTKRYA T 

Q96LR5 in FASTA format

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