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UniProtKB/Swiss-Prot entry Q969T4


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UB2E3_HUMAN
Primary accession number Q969T4
Secondary accession numbers Q5U0R7 Q7Z4W4
Integrated into Swiss-Prot on May 24, 2004
Sequence was last modified on December 1, 2001 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 66)
Name and origin of the protein
Protein name Ubiquitin-conjugating enzyme E2 E3
Synonyms EC 6.3.2.19
Ubiquitin-protein ligase E3
Ubiquitin carrier protein E3
Ubiquitin-conjugating enzyme E2-23 kDa
UbcH9
UbcM2
Gene name
Name: UBE2E3
Synonyms: UBCE4, UBCH9
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, AND MUTAGENESIS OF CYS-145.
TISSUE=Gastric adenocarcinoma;
PubMed=10343118 [NCBI, ExPASy, EBI, Israel, Japan]
Ito K., Kato S., Matsuda Y., Kimura M., Okano Y.;
"cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme.";
Cytogenet. Cell Genet. 84:99-104(1999).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Umbilical cord blood;
Shen Y., Ye M., Fu G., Zhou J., Zhang Q., Huang Q., Xu S., He K., Chen S., Mao M., Chen Z.;
"Human UbcM2 gene, complete cds.";
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ARG-201.
Xin Y.R., Yu L., Zhao S.Y.;
"Cloning of a new human cDNA homologous to Mus musculus ubiquitin-conjugating enzyme UbcM2.";
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature03466; PubMed=15815621 [NCBI, ExPASy, EBI, Israel, Japan]
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.;
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4.";
Nature 434:724-731(2005).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lung, and PNS;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[7]
SUBCELLULAR LOCATION.
DOI=10.1083/jcb.200406001; PubMed=15545318 [NCBI, ExPASy, EBI, Israel, Japan]
Plafker S.M., Plafker K.S., Weissman A.M., Macara I.G.;
"Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import.";
J. Cell Biol. 167:649-659(2004).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0404720101; PubMed=15302935 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB017644; BAA76544.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF085362; AAD40197.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF136176; AAP97266.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT019345; AAV38152.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC104076; AAY14882.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC003554; AAH03554.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC092407; AAH92407.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_006348.1; -.
NP_872619.1; -.
UniGene Hs.470804
3D structure databases
HSSP P15731; 1QCQ. [HSSP ENTRY / PDB]
SMR Q969T4; 60-207.
ModBase Q969T4.
Protein-protein interaction databases
IntAct Q969T4; 2.
PTM databases
PhosphoSite Q969T4; -.
Organism-specific databases
GeneCards GC02P181553; -.
H-InvDB HIX0002642; -.
HIX0056142; -.
HGNC HGNC:12479; UBE2E3.
GenAtlas UBE2E3.
HPA HPA003303; -.
MIM 604151; gene. [NCBI / EBI]
PharmGKB PA37129; -.
GeneCards Q969T4.
Gene expression databases
CleanEx HS_UBE2E3; -.
GermOnline ENSG00000170035; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0005730; Cellular component: nucleolus (inferred from direct assay from HPA).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0004842; Molecular function: ubiquitin-protein ligase activity (inferred from electronic annotation from EC).
GO:0043687; Biological process: post-translational protein modification (inferred from electronic annotation from InterPro).
GO:0001558; Biological process: regulation of cell growth (inferred from electronic annotation from UniProtKB-KW).
GO:0051246; Biological process: regulation of protein metabolic process (inferred from electronic annotation from InterPro).
GO:0006511; Biological process: ubiquitin-dependent protein catabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.
Gene3D G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
PANTHER PTHR11621; UBQ-conjugat_E2; 1.
Pfam PF00179; UQ_con; 1.
Pfam graphical view of domain structure.
ProDom PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00212; UBCc; 1.
SMART graphical view of domain structure.
PROSITE PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE Q969T4; -.
Genome annotation databases
Ensembl ENSG00000170035; Homo sapiens. [Contig view]
GeneID 10477; -.
KEGG hsa:10477; -.
Phylogenomic databases
HOGENOM Q969T4; -.
HOVERGEN Q969T4; -.
Other
NextBio 39740; -.
SOURCE UBE2E3; Homo sapiens.
ProtoNet Q969T4.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Growth regulation; Ligase; Nucleus; Phosphoprotein; Polymorphism; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   207  207     Ubiquitin-conjugating enzyme E2 E3. PRO_0000082474
ACT_SITE   145   145        Glycyl thioester intermediate. 
MOD_RES   12    12        Phosphoserine. 
MOD_RES   91    91        Phosphotyrosine (By similarity). 
VARIANT   201   201  1     W -> R (in dbSNP:rs2368192 [NCBI]). VAR_023392 [3D]
MUTAGEN   145   145        C->S: Loss of activity. 
CONFLICT   65    65        R -> K (in Ref. 3; AAP97266). 
CONFLICT   91    91        Y -> C (in Ref. 3; AAP97266). 
CONFLICT   101   101        P -> L (in Ref. 3; AAP97266). 
CONFLICT   134   134        Y -> D (in Ref. 3; AAP97266). 
CONFLICT   159   159        I -> V (in Ref. 3; AAP97266). 
CONFLICT   171   171        T -> M (in Ref. 3; AAP97266). 
CONFLICT   176   176        A -> V (in Ref. 3; AAP97266). 
Sequence information
Length: 207 AA [This is the length of the unprocessed precursor] Molecular weight: 22913 Da [This is the MW of the unprocessed precursor] CRC64: 821CB1382478DC9F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK LSSKTTAKLS 

        70         80         90        100        110        120 
TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP PGSVYEGGVF FLDITFSSDY 

       130        140        150        160        170        180 
PFKPPKVTFR TRIYHCNINS QGVICLDILK DNWSPALTIS KVLLSICSLL TDCNPADPLV 

       190        200 
GSIATQYLTN RAEHDRIARQ WTKRYAT 

Q969T4 in FASTA format

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