ID   NIFU2_ARATH             Reviewed;         235 AA.
AC   Q93W20; Q9LTX6;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   22-JUL-2008, entry version 33.
DE   RecName: Full=NifU-like protein 2, chloroplastic;
DE            Short=AtCNfu2;
DE            Short=AtCnfU-V;
DE   Flags: Precursor;
GN   Name=NIFU2; Synonyms=CNFU2, NFU2; OrderedLocusNames=At5g49940;
GN   ORFNames=K9P8.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP   AND GENE FAMILY.
RC   STRAIN=cv. Columbia;
RX   PubMed=12553879; DOI=10.1042/BJ20021946;
RA   Leon S., Touraine B., Ribot C., Briat J.-F., Lobreaux S.;
RT   "Iron-sulphur cluster assembly in plants: distinct NFU proteins in
RT   mitochondria and plastids from Arabidopsis thaliana.";
RL   Biochem. J. 371:823-830(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20181125; PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21932900; PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M.,
RA   Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T.,
RA   Shibata K., Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
RN   [5]
RP   FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=15031412; DOI=10.1105/tpc.020511;
RA   Yabe T., Morimoto K., Kikuchi S., Nishio K., Terashima I., Nakai M.;
RT   "The Arabidopsis chloroplastic NifU-like protein CnfU, which can act
RT   as an iron-sulfur cluster scaffold protein, is required for biogenesis
RT   of ferredoxin and photosystem I.";
RL   Plant Cell 16:993-1007(2004).
RN   [6]
RP   FUNCTION.
RX   PubMed=15361144; DOI=10.1111/j.1365-313X.2004.02189.x;
RA   Touraine B., Boutin J.-P., Marion-Poll A., Briat J.-F., Peltier G.,
RA   Lobreaux S.;
RT   "Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron-
RT   sulphur cluster assembly in Arabidopsis chloroplasts.";
RL   Plant J. 40:101-111(2004).
CC   -!- FUNCTION: Molecular scaffold for [Fe-S] cluster assembly of
CC       chloroplastic iron-sulfur proteins. Required for biogenesis of
CC       ferredoxin, a major photosynthetic electron carrier containing
CC       [2Fe-2S] cluster. Required for the assembly of photosystem I
CC       complex.
CC   -!- COFACTOR: Binds 1 [2Fe-2S] cluster per subunit.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences;
CC       Name=1;
CC         IsoId=Q93W20-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in leaves and floral
CC       stalks. Ubiquitous (at protein level).
CC   -!- MISCELLANEOUS: Plants lacking NFU2 are dwarf with faint pale-green
CC       leaves, decreased amount of ferredoxin and impaired photosystem I
CC       accumulation.
CC   -!- SIMILARITY: Belongs to the nifU family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA97015.1; Type=Erroneous gene model prediction; Note=The predicted gene At5g49940 has been split into 2 genes: At5g49940 and At5g49945;
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DR   EMBL; AJ512934; CAD55559.1; -; mRNA.
DR   EMBL; AB024032; BAA97015.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AF370353; AAK44168.1; -; mRNA.
DR   EMBL; AF428399; AAL16167.1; -; mRNA.
DR   EMBL; AY062984; AAL34158.1; -; mRNA.
DR   EMBL; AK118652; BAC43248.1; -; mRNA.
DR   RefSeq; NP_568715.1; -.
DR   UniGene; At.26219; -.
DR   GeneID; 835057; -.
DR   GenomeReviews; BA000015_GR; AT5G49940.
DR   NMPDR; fig|3702.1.peg.26841; -.
DR   GeneFarm; 3943; 402.
DR   TAIR; At5g49940; -.
DR   ArrayExpress; Q93W20; -.
DR   GermOnline; AT5G49940; Arabidopsis thaliana.
DR   InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR   Pfam; PF01106; NifU; 2.
DR   ProDom; PD002830; NifU_C; 2.
PE   1: Evidence at protein level;
KW   2Fe-2S; Alternative splicing; Chloroplast; Complete proteome; Iron;
KW   Iron-sulfur; Metal-binding; Plastid; Transit peptide.
FT   TRANSIT       1     16       Chloroplast (Potential).
FT   CHAIN        17    235       NifU-like protein 2, chloroplastic.
FT                                /FTId=PRO_0000247613.
SQ   SEQUENCE   235 AA;  25620 MW;  6443A3A407D8A3A8 CRC64;
     MQLLTLNPAA ISRTPPQAID PSSSSSLLLP FPQILSSQRA LGLVARPCNP LRRGLSRFLS
     SRQLFRRSKV VKAVATPDPI LEVPLTEENV ESVLDEIRPY LMSDGGNVAL HEIDGNIVRV
     KLQGACGSCP SSTMTMKMGI ERRLMEKIPE IVAVEALPDE ETGLELNEEN IEKVLEEIRP
     YLIGTADGSL DLVEIEDPIV KIRITGPAAG VMTVRVAVTQ KLREKIPSIA AVQLI
//