ID   LDH1_LISIN              Reviewed;         313 AA.
AC   Q92F65; Q8VTS6;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-NOV-2008, entry version 53.
DE   RecName: Full=L-lactate dehydrogenase 1;
DE            Short=L-LDH 1;
DE            EC=1.1.1.27;
GN   Name=ldh1; Synonyms=ldh; OrderedLocusNames=lin0242;
OS   Listeria innocua.
OC   Bacteria; Firmicutes; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=1642;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIP 11262 / Serovar 6a;
RX   MEDLINE=21537279; PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A.,
RA   Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T.,
RA   Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P.,
RA   Domann E., Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O.,
RA   Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P.,
RA   Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D.,
RA   Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G.,
RA   Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H.,
RA   Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R.,
RA   Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A.,
RA   Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 223-313.
RC   STRAIN=dd644;
RX   MEDLINE=21539548; PubMed=11683362; DOI=10.1007/s002840010300;
RA   Cai S., Wiedmann M.;
RT   "Characterization of the prfA virulence gene cluster insertion site in
RT   non-hemolytic Listeria spp.: probing the evolution of the Listeria
RT   virulence gene island.";
RL   Curr. Microbiol. 43:271-277(2001).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AL596164; CAC95475.1; -; Genomic_DNA.
DR   EMBL; AF322004; AAL36598.1; -; Genomic_DNA.
DR   PIR; AC1463; AC1463.
DR   RefSeq; NP_469587.1; -.
DR   HSSP; P00344; 2LDB.
DR   GeneID; 1128650; -.
DR   GenomeReviews; AL592022_GR; lin0242.
DR   KEGG; lin:lin0242; -.
DR   NMPDR; fig|272626.1.peg.240; -.
DR   ListiList; LIN00242; -.
DR   HOGENOM; Q92F65; -.
DR   BioCyc; LINN272626:LIN0242-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00488; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase;
KW   Phosphoprotein.
FT   CHAIN         1    313       L-lactate dehydrogenase 1.
FT                                /FTId=PRO_0000168361.
FT   NP_BIND      13     41       NAD (By similarity).
FT   ACT_SITE    176    176       Proton acceptor (By similarity).
FT   BINDING      89     89       Substrate (By similarity).
FT   BINDING     121    121       NAD or substrate (By similarity).
FT   BINDING     152    152       Substrate (By similarity).
FT   BINDING     227    227       Substrate (By similarity).
FT   MOD_RES     218    218       Phosphotyrosine (By similarity).
FT   CONFLICT    223    223       K -> L (in Ref. 2; AAL36598).
SQ   SEQUENCE   313 AA;  34218 MW;  E95D336CB317E4C1 CRC64;
     MKDHQKIILV GDGAVGSSYA FACVNLSIGQ EFGIIDIDKD RTIGDAMDLS HAVPFSTPKK
     IYSANYSDCH DADLVVVTAG TAQKPGETRL DLVNRNIKIM KGIVDEVMAS GFDGIFLIAS
     NPVDILTYAT WKFSGLPKER VIGSGTSLDT ARFRMSIADY LKVDARNVHG YILGEHGDTQ
     FPAWSHTTVG GLPITEWINE DEQGAMDTIF VSVRDAAYEI INKKGATFYG VAAALARITK
     AILNNENAIL PLSVYLDGHY GMNDIYIGAP AVVNRQGVRH IVEMNLNDKE KEQMKNSADT
     LKKVLDDAMK QID
//