ID   EID1_ARATH              Reviewed;         336 AA.
AC   Q8LEA8; O81289; Q0WMI9;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2005, sequence version 2.
DT   16-DEC-2008, entry version 44.
DE   RecName: Full=Phytochrome A-associated F-box protein;
DE   AltName: Full=Empfindlicher im dunkelroten Licht protein 1;
GN   Name=EID1; OrderedLocusNames=At4g02440; ORFNames=T14P8.22;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION.
RX   MEDLINE=20225526; PubMed=10760243; DOI=10.1105/tpc.12.4.547;
RA   Bueche C., Poppe C., Schaefer E., Kretsch T.;
RT   "eid1: a new Arabidopsis mutant hypersensitive in phytochrome A-
RT   dependent high-irradiance responses.";
RL   Plant Cell 12:547-558(2000).
RN   [6]
RP   SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, INTERACTION WITH
RP   SKP1A AND SKP1B, DOMAIN, AND MUTAGENESIS OF PRO-10; GLY-101 AND
RP   197-ARG-LYS-198.
RX   MEDLINE=21216578; PubMed=11316788; DOI=10.1101/gad.197201;
RA   Dieterle M., Zhou Y.-C., Schaefer E., Funk M., Kretsch T.;
RT   "EID1, an F-box protein involved in phytochrome A-specific light
RT   signaling.";
RL   Genes Dev. 15:939-944(2001).
CC   -!- FUNCTION: Negative regulator of the phyA signaling pathway that
CC       shifts the responsiveness of the phyA signaling system associated
CC       with hypocotyl elongation from red to far-red wavelength.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Probable component of an E3 ubiquitin ligase SCF complex.
CC       Interacts with SKP1A and SKP1B.
CC   -!- INTERACTION:
CC       Q39255:SKP1A; NbExp=2; IntAct=EBI-687388, EBI-532357;
CC       Q9FHW7:SKP1B; NbExp=2; IntAct=EBI-687388, EBI-604076;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DOMAIN: The F-box domain is required for the interaction with
CC       SKP1A and SKP1B.
CC   -!- MISCELLANEOUS: 'Empfindlicher im dunkelroten licht' means
CC       sensitive to far-red light in German.
CC   -!- SIMILARITY: Contains 1 F-box domain.
CC   -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database
CC       for the ubiquitin/26S proteasome proteolytic pathway in plants;
CC       URL="http://plantsubq.genomics.purdue.edu/";
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DR   EMBL; AF069298; AAC19270.1; -; Genomic_DNA.
DR   EMBL; AL161494; CAB80737.1; -; Genomic_DNA.
DR   EMBL; BT002038; AAN72049.1; -; mRNA.
DR   EMBL; BT008731; AAP42744.1; -; mRNA.
DR   EMBL; AK229833; BAF01662.1; -; mRNA.
DR   EMBL; AY085530; AAM62754.1; -; mRNA.
DR   PIR; T01306; T01306.
DR   RefSeq; NP_192153.1; -.
DR   UniGene; At.43977; -.
DR   IntAct; Q8LEA8; 11.
DR   PRIDE; Q8LEA8; -.
DR   GeneID; 828008; -.
DR   GenomeReviews; CT486007_GR; AT4G02440.
DR   KEGG; ath:AT4G02440; -.
DR   NMPDR; fig|3702.1.peg.18057; -.
DR   TAIR; At4g02440; -.
DR   GermOnline; AT4G02440; Arabidopsis thaliana.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0048366; P:leaf development; IMP:TAIR.
DR   GO; GO:0048573; P:photoperiodism, flowering; IMP:TAIR.
DR   GO; GO:0010017; P:red or far red light signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0009585; P:red, far-red light phototransduction; IEA:UniProtKB-KW.
DR   GO; GO:0010099; P:regulation of photomorphogenesis; IMP:TAIR.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001810; F-box.
DR   Pfam; PF00646; F-box; 1.
DR   PROSITE; PS50181; FBOX; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Nucleus; Phytochrome signaling pathway;
KW   Ubl conjugation pathway.
FT   CHAIN         1    336       Phytochrome A-associated F-box protein.
FT                                /FTId=PRO_0000119961.
FT   DOMAIN        3     55       F-box.
FT   MOTIF       197    201       Nuclear localization signal.
FT   MUTAGEN      10     10       P->A: Disrupts interaction with SKP1A and
FT                                SKP1B.
FT   MUTAGEN     101    101       G->C: In eid1-2; enhances sensitivity to
FT                                red and far-red light.
FT   MUTAGEN     197    198       RK->SE: Cytoplasmic localization instead
FT                                of nuclear.
FT   CONFLICT     16     16       N -> K (in Ref. 4; AAM62754).
FT   CONFLICT     71     71       A -> AA (in Ref. 4; AAM62754).
SQ   SEQUENCE   336 AA;  37398 MW;  0E4231CCF243487C CRC64;
     MAESVFSCIP EDVVFNIFFK LQDDPRNWAR LACVCTKFSS IVRNVCCKTQ CYSAIPTVIS
     DLLPLPPSAA ASASSSTAAD SSLTPPGGWA SLYKLAVCCP GLFHAGILLE NSDFGLEREL
     GPDQNLDPKP TTTDLALNDE EVSKPVGSGL ETTSFWSLYD DLYTDTIPAP PPEDSIDDQE
     EEIETSEIRP GRDLPVRKRR KICRSLGSHL ASGGWNLSRE QGNKLLASRF RGDCLYICNW
     PGCIHVEEKR NYMLFRGVFK DFKRSRVWRT INDGNRSKTS GLKCAFCLCD ETWDLHSSFC
     LRRVFGFHDD GEPVVRAYVC ENGHVSGAWT ALPLYT
//