ID   AFR_ARATH               Reviewed;         372 AA.
AC   Q8LAW2; Q9SJB1;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   16-DEC-2008, entry version 42.
DE   RecName: Full=Protein AFR;
DE   AltName: Full=Protein ATTENUATED FAR-RED RESPONSE;
GN   Name=AFR; OrderedLocusNames=At2g24540; ORFNames=F25P17.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   INDUCTION.
RX   MEDLINE=20568398; PubMed=11118138; DOI=10.1126/science.290.5499.2110;
RA   Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T.,
RA   Wang X., Kreps J.A., Kay S.A.;
RT   "Orchestrated transcription of key pathways in Arabidopsis by the
RT   circadian clock.";
RL   Science 290:2110-2113(2000).
RN   [5]
RP   FUNCTION, INDUCTION, AND INTERACTION WITH SKP1A.
RX   PubMed=14653999; DOI=10.1016/j.cub.2003.11.019;
RA   Harmon F.G., Kay S.A.;
RT   "The F box protein AFR is a positive regulator of phytochrome A-
RT   mediated light signaling.";
RL   Curr. Biol. 13:2091-2096(2003).
CC   -!- FUNCTION: Part of the phyA-mediated signaling transduction pathway
CC       leading to the regulation of gene expression and hypocotyls
CC       elongation in response to red and far-red light exposure.
CC   -!- SUBUNIT: Interacts with SKP1A. May be part of a E3 ubiquitin
CC       ligase SCF(AFR) complex.
CC   -!- INTERACTION:
CC       P25854:CAM1; NbExp=1; IntAct=EBI-604438, EBI-1235664;
CC       P59220:CAM7; NbExp=1; IntAct=EBI-604438, EBI-1236031;
CC       P30187:CML10; NbExp=1; IntAct=EBI-604438, EBI-1238722;
CC       Q9S744:CML9; NbExp=1; IntAct=EBI-604438, EBI-1236048;
CC       Q39255:SKP1A; NbExp=3; IntAct=EBI-604438, EBI-532357;
CC   -!- INDUCTION: Rhythmic expression with highest levels at the end of
CC       the dark phase, just before the transition to light. Induction by
CC       far-red light requires phyA.
CC   -!- DOMAIN: The kelch repeats form a beta-propeller structure involved
CC       in protein-protein interaction.
CC   -!- DOMAIN: The F-box is required for the interaction with SKP1A.
CC   -!- SIMILARITY: Contains 1 F-box domain.
CC   -!- SIMILARITY: Contains 5 Kelch repeats.
CC   -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database
CC       for the ubiquitin/26S proteasome proteolytic pathway in plants;
CC       URL="http://plantsubq.genomics.purdue.edu/";
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DR   EMBL; AC006954; AAD23891.1; -; Genomic_DNA.
DR   EMBL; AY072009; AAL57704.1; -; mRNA.
DR   EMBL; BT000847; AAN38684.1; -; mRNA.
DR   EMBL; AY087570; AAM65112.1; -; mRNA.
DR   PIR; H84637; H84637.
DR   RefSeq; NP_565572.1; -.
DR   UniGene; At.28274; -.
DR   IntAct; Q8LAW2; 6.
DR   PRIDE; Q8LAW2; -.
DR   GeneID; 816990; -.
DR   GenomeReviews; CT485783_GR; AT2G24540.
DR   KEGG; ath:AT2G24540; -.
DR   NMPDR; fig|3702.1.peg.9478; -.
DR   TAIR; At2g24540; -.
DR   GermOnline; AT2G24540; Arabidopsis thaliana.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0010017; P:red or far red light signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0009585; P:red, far-red light phototransduction; IEA:UniProtKB-KW.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001810; F-box.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR013089; Kelch_related.
DR   Gene3D; G3DSA:2.120.10.80; Kelch-typ_b-propeller; 1.
DR   PANTHER; PTHR23230; Kelch_related; 1.
DR   Pfam; PF00646; F-box; 1.
DR   Pfam; PF01344; Kelch_1; 2.
DR   SMART; SM00256; FBOX; 1.
DR   SMART; SM00612; Kelch; 2.
DR   PROSITE; PS50181; FBOX; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Kelch repeat; Phytochrome signaling pathway;
KW   Repeat; Ubl conjugation pathway.
FT   CHAIN         1    372       Protein AFR.
FT                                /FTId=PRO_0000119959.
FT   DOMAIN       29     74       F-box.
FT   REPEAT       80    126       Kelch 1.
FT   REPEAT      135    178       Kelch 2.
FT   REPEAT      179    227       Kelch 3.
FT   REPEAT      229    276       Kelch 4.
FT   REPEAT      279    325       Kelch 5.
FT   CONFLICT    130    130       M -> I (in Ref. 3; AAM65112).
FT   CONFLICT    246    246       Q -> E (in Ref. 3; AAM65112).
FT   CONFLICT    307    307       Q -> P (in Ref. 3; AAM65112).
FT   CONFLICT    351    351       R -> K (in Ref. 3; AAM65112).
SQ   SEQUENCE   372 AA;  41278 MW;  0F48D9747B900634 CRC64;
     MAEQETTSNI NTINDQAEEE TRTKSQPLIS GLPNDIAELC LLRLPYPYHA LYRSVSSSWN
     KTITNPRFLF SKQSLSISSP YLFVFAFNKS TARIQWQSLD LASGRWFVLP PMPNSFTKIS
     SPHALSCASM PRQGKLFVLG GGDVNRSAVV YTALTNRWSC ISPMMSPRTY FVSGNVNGKI
     MAVGGSVGGN GEATTEVESY DPDNDTWTVV KKLPMVLAKY DSAVIGKEMC VTEGWAWPFM
     FPPMGQVYDS DEGTWREMSG GMKEGWTGVS VVIRDRLFVI SEHGDFPMKV YCSDDDTWRY
     VSGEKLQGEK MRRPFAVTGA DDRVFVVASG INVAEGRVSE GQNGDFSVEW RMVSSPKSSI
     QFSPASCHVL YV
//