FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).
CATALYTIC ACTIVITY: NADH + quinone = NAD⁺ + quinol.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
SUBUNIT: NDH-1 is composed of 13 different subunits. Subunits nuoB, CD, E, F, and G constitute the peripheral sector of the complex (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
SIMILARITY: Belongs to the complex I 20 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE013218; AAM67716.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_660505.1; -.

3D structure databases

ModBase

Q8K9Y6.

Enzyme and pathway databases

BioCyc

BAPH198804:BUSG148-MON; -.

Ontologies

GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008137;	Molecular function: NADH dehydrogenase (ubiquinone) activity (inferred from electronic annotation from InterPro).
GO:0048038;	Molecular function: quinone binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006120;	Biological process: mitochondrial electron transport, NADH to ubiquinone (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

HAMAP

MF_01356; -; 1.
PBIL [Tree]

InterPro

IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.

PANTHER

PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.

Pfam

PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01957; nuoB_fam; 1.

PROSITE

PS01150; COMPLEX1_20K; 1.

Genome annotation databases

GeneID

1005965; -.

GenomeReviews

AE013218_GR; BUsg_148.

KEGG

bas:BUsg148; -.

Phylogenomic databases

HOGENOM

Q8K9Y6; -.

Genome annotation databases

CMR

Q8K9Y6; BUsg_148.

Other

ProtoNet

Q8K9Y6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Cell membrane; Complete proteome; Iron; Iron-sulfur; Membrane; Metal-binding; NAD; Oxidoreductase; Quinone; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 223`	`223`	`NADH-quinone oxidoreductase subunit B.`	`PRO_0000118770`
`METAL`	`66 66`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`67 67`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`132 132`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`161 161`		`Iron-sulfur (4Fe-4S) (Potential).`

Sequence information

Length: 223 AA [This is the length of the unprocessed precursor]

Molecular weight: 25497 Da [This is the MW of the unprocessed precursor]

CRC64: 9883840771B302BF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNYTLTRAER KSQRHYPNET TQSTIDPIEG YLKKNILMGK VTKLLHKIVN WGRKNSLWPY 

        70         80         90        100        110        120 
NFGLSCCYVE MVTAFTSVHD VARFGSEVLR ASPRQADVMV IAGTPFIKMA PVIQRLYDQM 

       130        140        150        160        170        180 
LEPKWVISMG ACANSGGMYD IYSVVQGVDK FLPVDIYIPG CPPRPEAYIH ALTLLQKTIN 

       190        200        210        220 
EERRPLSWVV GEQGIYRKKM LSEKIQNRKK RISVVNLPTS EKI

Q8K9Y6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools