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UniProtKB/Swiss-Prot entry Q8JTG9

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_ABLVH
Primary accession number Q8JTG9
Secondary accession numbers None
Integrated into Swiss-Prot on July 10, 2007
Sequence was last modified on October 1, 2002 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 25)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Australian bat lyssavirus (isolate Human/AUS/1998) (ABLV) [TaxID: 446562] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Lyssavirus.
Virus hosts Homo sapiens (Human) [TaxID: 9606]
Pteropus alecto (Black flying fox) [TaxID: 9402]
Pteropus conspicillatus (Spectacled flying fox) [TaxID: 328804]
Pteropus poliocephalus (Grey-headed flying fox) [TaxID: 9403]
Pteropus scapulatus (Little red flying fox) [TaxID: 94117]
Saccolaimus [TaxID: 446909]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1006/viro.2002.1417; PubMed=12083841 [NCBI, ExPASy, EBI, Israel, Japan]
Warrilow D., Smith I.L., Harrower B., Smith G.A.;
"Sequence analysis of an isolate from a fatal human infection of Australian bat lyssavirus.";
Virology 297:109-119(2002).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein to form the functional polymerase (By similarity).
  • SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the rhabdoviruses protein L family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF418014; AAN05310.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
ModBase Q8JTG9.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037546; RNA_pol_RhabdoV_sub; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
Other
ProtoNet Q8JTG9.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2127  2127     Large structural protein. PRO_0000294414
DOMAIN   611    799  189     RdRp catalytic. 
REGION   1562   2127  566     Interaction with P protein (By similarity). 
Sequence information
Length: 2127 AA [This is the length of the unprocessed precursor] Molecular weight: 242676 Da [This is the MW of the unprocessed precursor] CRC64: E3777CFCDA404F4E [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MIDPGEVYDD PIDPVEPEPE LKNNTVIPNI LRNSDYNLNS PLIEDSARLM LEWLTTGNRP 

        70         80         90        100        110        120 
GRLTLTDNCI RSYKTLKCYF RKVDVGSLKV GGLAAQAMIS LWLHGEHSES NRSRKCLSDL 

       130        140        150        160        170        180 
TQFYQKSSPI EKLLNYTLGN RGLRIPPEGV LFCLKKVDYD RAFGRYLANI YASYLFFHVI 

       190        200        210        220        230        240 
ILYMNALDWD EEKTILALWK DLSSVDIKKD QVKFRDPIWG SLIVTKDFVY SQNANCLFDR 

       250        260        270        280        290        300 
NYTLMLKDLF LSRFNSLLIL LSPPEPRYSD DLISQLCQLY IAGDNVLSMC GNSGYDVIKM 

       310        320        330        340        350        360 
LEPYVVNSLV QRAEGFRPLI HSLGDFPLFI RDKVGQLEGT FGPSARKFFQ VLDQFDNIHD 

       370        380        390        400        410        420 
LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH IKKVIDGTYQ ECLASDLAKR VLRWGFDKYS 

       430        440        450        460        470        480 
KWYLDPKLLA QDHPLTPYIR TQTWPPKHIV DLVGNTWHRL PITQIFEIPE SMDPSEILDD 

       490        500        510        520        530        540 
KSHSLTRTKL ASWLAENRGG PVPSEKVIIT ALSKPPVNPR EFLKSIDLGG LPDDDLIIGL 

       550        560        570        580        590        600 
KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 

       610        620        630        640        650        660 
TGQGLQDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDQ VFGLKKVFSR THEFFQKSWV 

       670        680        690        700        710        720 
YYSDRSDLIG LWEDQIYCLD MSDGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 

       730        740        750        760        770        780 
RTKILAQGDN QVLCPTYMLS SGLSQEGLLY ELESISRNAL SIYRAIEDGA SKLGLIIKKE 

       790        800        810        820        830        840 
ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 

       850        860        870        880        890        900 
SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAD GDDFLLAMSR IIYLDPSLGG 

       910        920        930        940        950        960 
VSGMSLGRFH IRQFSDPVSE GLSFWKEIWS SSSEPWIHSL CQEAGNPDLG DRSLESFTRL 

       970        980        990       1000       1010       1020 
LEDPTTLNIR GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSI 

      1030       1040       1050       1060       1070       1080 
EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRSLEESFY NSEIHGINRM 

      1090       1100       1110       1120       1130       1140 
TQVPQRIGRV WSCSSERADL LREISWGRKV VGTTVPHPSE MLTLLPKSSI SCVCGPTGSE 

      1150       1160       1170       1180       1190       1200 
NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 

      1210       1220       1230       1240       1250       1260 
WFIARDSNLA QTLIKNIISL TGPQFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSICPNL 

      1270       1280       1290       1300       1310       1320 
LSHISVSTDT MSDLTQDGRN FDFMFQPLML YAQTWTSELV QKDIRLKDST FHWHLRCQKC 

      1330       1340       1350       1360       1370       1380 
IRSIDDITLD TSQVFEFPDV SKRISRMVSG AVPQFQKLPE VYLKPGHFDS LCGKDKSRHI 

      1390       1400       1410       1420       1430       1440 
GSAQGLLYSI LVATHDPGYN DGTIFPVNIY SKISPKDYLR GLARGVLIGS SICFLTRMTN 

      1450       1460       1470       1480       1490       1500 
ININRPLELI SGVISYILLR LDNHPSLYVM LRESSLRSEI FSIPQKIPAA YPTTMKEGNR 

      1510       1520       1530       1540       1550       1560 
SVLCYLQHVL RYERDIITSS PENDWLWIFS DFRSTKMTYL TLITYQSHIL LQRIERSLSK 

      1570       1580       1590       1600       1610       1620 
KMRADLRQLS SLMRQVLGGH GEDSLDSGED IQRLLRDALQ RTRWVDQEVR HAAKTMTGDH 

      1630       1640       1650       1660       1670       1680 
SPSKKTSRKA GCSEWICSAQ QVAISTSSNP APVSEMDIRT LSRKLQNPLI SGLRVVQWAT 

      1690       1700       1710       1720       1730       1740 
GAHYKLKPIL DDLNAYPSFC LVVGDGSGGI SRTVLNMFPD AKLVFNSLLE VSDLMASGTH 

      1750       1760       1770       1780       1790       1800 
PLPPSAIMSG GDDIVSRVVD FESIWEKPSD LRNLSTWRYF QSVQSKLNMS YDLIICDAEV 

      1810       1820       1830       1840       1850       1860 
TDIISVNKIT LLMSDFVLSI DGPLDLIFKS YGTMLVNPDY KAIQHLSRAF PKATGYITQL 

      1870       1880       1890       1900       1910       1920 
TSSFSSELYL RFSKRGKFFR DVEYLTSSTL REMSLVLFNC SSPKSELQRA RSLNYQDLIR 

      1930       1940       1950       1960       1970       1980 
GFPEEIVSNP YNEMIITLID SEVESFLVHK MVDDLELQRG TLSKMSIIIA IVIVYSNRVF 

      1990       2000       2010       2020       2030       2040 
NVSKPLSDPV FYPPSDPKIL RHFNICCSTM LYLSTILGDV PNFARLHELY NSPITYYFKK 

      2050       2060       2070       2080       2090       2100 
QIIRGSIYLS WSWSDDTSVF KRVSCNSNLS LSSHWIRLIY KIIKTTRFTG SHVDLSKEVE 

      2110       2120 
KHLKGYNRWI TFNDVRSRSS LLDYSCL
Q8JTG9 in FASTA format

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