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UniProtKB/Swiss-Prot entry Q8JPX5

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_EBORR
Primary accession number Q8JPX5
Secondary accession number Q5UAK4
Integrated into Swiss-Prot on July 11, 2006
Sequence was last modified on October 1, 2002 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 27)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus) [TaxID: 386032] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Filoviridae; Ebola-like viruses.
Virus hosts Epomops franqueti (Franquet's epauleted bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/S0168-1702(02)00087-4; PubMed=12191779 [NCBI, ExPASy, EBI, Israel, Japan]
Groseth A., Stroeher U., Theriault S., Feldmann H.;
"Molecular characterization of an isolate from the 1989/90 epizootic of Ebola virus Reston among macaques imported into the United States.";
Virus Res. 87:155-163(2002).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
STRAIN=Isolate Pennsylvania-89;
DOI=10.1016/j.virol.2004.11.018; PubMed=15661171 [NCBI, ExPASy, EBI, Israel, Japan]
Boehmann Y., Enterlein S., Randolf A., Muehlberger E.I.;
"A reconstituted replication and transcription system for Ebola virus Reston and comparison with Ebola virus Zaire.";
Virology 332:406-417(2005).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBCELLULAR LOCATION: Cytoplasm. Virion (By similarity).
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF522874; AAN04454.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY769362; AAV48581.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_690587.1; -.
3D structure databases
ModBase Q8JPX5.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR017235; RNA-dir_RNA_pol_filovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037548; RNA_pol_Filoviridae; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
GeneID 955191; -.
Other
ProtoNet Q8JPX5.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2212  2212     Large structural protein. PRO_0000245048
DOMAIN   625    809  185     RdRp catalytic. 
VARIANT   192    192  1     K -> R (in strain: Isolate Pennsylvania-89). 
VARIANT   324    324  1     H -> R (in strain: Isolate Pennsylvania-89). 
VARIANT   442    442  1     F -> V (in strain: Isolate Pennsylvania-89). 
VARIANT   600    600  1     E -> G (in strain: Isolate Pennsylvania-89). 
VARIANT   755    755  1     K -> E (in strain: Isolate Pennsylvania-89). 
VARIANT   1374   1374  1     D -> G (in strain: Isolate Pennsylvania-89). 
VARIANT   1487   1487  1     E -> D (in strain: Isolate Pennsylvania-89). 
VARIANT   1679   1679  1     E -> A (in strain: Isolate Pennsylvania-89). 
VARIANT   1896   1898  3     ASQ -> SSH (in strain: Isolate Pennsylvania-89). 
VARIANT   1909   1909  1     S -> SS (in strain: Isolate Pennsylvania-89). 
Sequence information
Length: 2212 AA [This is the length of the unprocessed precursor] Molecular weight: 252549 Da [This is the MW of the unprocessed precursor] CRC64: C28CE580881136E3 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MATQHTQYPD ARLSSPIVLD QCDLVTRACG LYSSYSLNPQ LRQCKLPKHI YRLKFDTIVS 

        70         80         90        100        110        120 
KFLSDTPVAT LPIDYLVPIL LRSLTGHGDR PLTPTCNQFL DEIINYTLHD AAFLDYYLKA 

       130        140        150        160        170        180 
TGAQDHLTNI ATREKLKNEI LNNDYVHQLF FWHDLSILAR RGRLNRGNNR STWFVHDEFI 

       190        200        210        220        230        240 
DILGYGDYIF WKIPLSLLPV TIDGVPHAAT DWYQPTLFKE SILGHSQILS VSTAEILIMC 

       250        260        270        280        290        300 
KDIITCRFNT SLIASIAKLE DVDVSDYPDP SDILKIYNAG DYVISILGSE GYKIIKYLEP 

       310        320        330        340        350        360 
LCLAKIQLCS KFTERKGRFL TQMHLSVIND LRELISNRRL KDYQQEKIRD FHKILLQLQL 

       370        380        390        400        410        420 
SPQQFCELFS VQKHWGHPIL HSEKAIQKVK RHATILKALR PNVIFETYCV FKYNIAKHYF 

       430        440        450        460        470        480 
DSQGTWYSVI SDRNLTPGLN SFIKRNHFPS LPMIKDLLWE FYHLNHPPLF STKVISDLSI 

       490        500        510        520        530        540 
FIKDRATAVE QTCWDAVFEP NVLGYNPPNK FSTKRVPEQF LEQEDFSIES VLNYAQELHY 

       550        560        570        580        590        600 
LLPQNRNFSF SLKEKELNIG RTFGKLPYLT RNVQTLCEAL LADGLAKAFP SNMMVVTERE 

       610        620        630        640        650        660 
QKESLLHQAS WHHTSDDFGE NATVRGSSFV TDLEKYNLAF RYEFTAPFIE YCNHCYGVRN 

       670        680        690        700        710        720 
VFNWMHYLIP QCYMHVSDYY NPPHNVNLSN REYPPEGPSS YRGHLGGIEG LQQKLWTSIS 

       730        740        750        760        770        780 
CAQISLVEIK TGFKLRSAVM GDNQCITVLS VFPLKTDPEE QEQSAEDNAA RVAASLAKVT 

       790        800        810        820        830        840 
SACGIFLKPD ETFVHSGFIY FGKKQYLNGV QLPQSLKTAA RMAPLSDAIF DDLQGTLASI 

       850        860        870        880        890        900 
GTAFERAISE TRHILPCRIV AAFHTYFAVR ILQYHHLGFN KGIDLGQLSL SKPLDYGTIT 

       910        920        930        940        950        960 
LTLAVPQVLG GLSFLNPEKC FYRNFGDPVT SGLFQLRVYL EMVNMKDLFC PLISKNPGNC 

       970        980        990       1000       1010       1020 
SAIDFVLNPS GLNVPGSQDL TSFLRQIVRR SITLTARNKL INTLFHASAD LEDEMVCKWL 

      1030       1040       1050       1060       1070       1080 
LSSNPVMSRF AADIFSRTPS GKRLQILGYL EGTRTLLASK IINNNSETPV LDKLRKITLQ 

      1090       1100       1110       1120       1130       1140 
RWNLWFSYLD HCDQLLADAL QKISCTVDLA QILREYTWSH ILEGRSLIGA TLPCMVEQFK 

      1150       1160       1170       1180       1190       1200 
VKWLGQYEPC PECLNKKGSN AYVSVAVKDQ VVSAWPNTSR ISWTIGSGVP YIGSRTEDKI 

      1210       1220       1230       1240       1250       1260 
GQPAIKPRCP SSALKEAIEL ASRLTWVTQG GSNSEQLIRP FLEARVNLSV SEVLQMTPSH 

      1270       1280       1290       1300       1310       1320 
YSGNIVHRYN DQYSPHSFMA NRMSNTATRL IVSTNTLGEF SGGGQAARDS NIIFQNVINL 

      1330       1340       1350       1360       1370       1380 
AVALYDIRFR NTNTSDIRHN RAHLHLTECC TKEVPAQYLT YTSALNLDLS RYRDNELIYD 

      1390       1400       1410       1420       1430       1440 
SNPLKGGLNC NLTIDSPLVK GPRLNMIEDD LLRFPHLSGW ELAKTVVQSI ISDNSNSSTD 

      1450       1460       1470       1480       1490       1500 
PISSGETRSF TTHFLTYPQI GLLYSFGAVL CFYLGNTILW TKKLDYEQFL YYLHNQLHNL 

      1510       1520       1530       1540       1550       1560 
PHRALRVFKP TFKHASVMSR LMEIDSNFSI YIGGTSGDRG LSDAARLFLR TAIASFLQFL 

      1570       1580       1590       1600       1610       1620 
KSWIIDRQKT IPLWIVYPLE GQQPESINEF LHKILGLLKQ GPKSIPKEVS IQNDGHLDLA 

      1630       1640       1650       1660       1670       1680 
ENNYVYNSKS TASNFFHASL AYWRSRKSRK TQDHNDFSRG DGTLTEPVRK FSSNHQSDEK 

      1690       1700       1710       1720       1730       1740 
YYNVTCGKSP KPQERKDFSQ YRLSNNGQTM SNHRKKGKFH KWNPCKMLME SQRGTVLTEG 

      1750       1760       1770       1780       1790       1800 
DYFQNNTPPT DDVSSPHRLI LPFFKLGNHN HAHDQDAQEL MNQNIKQYLH QLRSMLDTTI 

      1810       1820       1830       1840       1850       1860 
YCRFTGIVSS MHYKLDEVLL EYNSFDSAIT LAEGEGSGAL LLLQKYSTRL LFLNTLATEH 

      1870       1880       1890       1900       1910       1920 
SIESEVVSGF STPRMLLPIM QKVHEGQVTV ILNNSASQIT DITSSMWLSN QKYNLPCQVE 

      1930       1940       1950       1960       1970       1980 
IIMMDAETTE NLNRSQLYRA VYNLILDHID PQYLKVVVLK VFLSDIEGIL WINDYLAPLF 

      1990       2000       2010       2020       2030       2040 
GAGYLIKPIT SSARSSEWYL CLSNLISTNR RSAHQTHKAC LGVIRDALQA QVQRGVYWLS 

      2050       2060       2070       2080       2090       2100 
HIAQYATKNL HCEYIGLGFP SLEKVLYHRY NLVDTGLGPL SSVIRHLTNL QAEIRDLVLD 

      2110       2120       2130       2140       2150       2160 
YNLMRESRTQ TYHFIKTAKG RITKLVNDFL KFSLIVQALK NNSSWYTELK KLPEVINVCN 

      2170       2180       2190       2200       2210 
RFYHTHNCEC QEKFFVQTLY LQRLRDAEIK LIERLTGLMR FYPEGLIYSN HT
Q8JPX5 in FASTA format

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