NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=DSM 14371 / JCM 11309 / KCTC 3954 / HTE831;
DOI=10.1093/nar/gkf526; PubMed=12235376 [NCBI, ExPASy, EBI, Israel, Japan]
Takami H., Takaki Y., Uchiyama I.;
"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments.";
Nucleic Acids Res. 30:3927-3935(2002).

Comments

FUNCTION: Initiates the rapid degradation of small, acid-soluble proteins during spore germination (By similarity).
CATALYTIC ACTIVITY: Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala.
SUBUNIT: Homotetramer (By similarity).
PTM: Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination (By similarity).
SIMILARITY: Belongs to the peptidase A25 family [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000028; BAC13931.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_692896.1; -.

3D structure databases

HSSP

P22321; 1C8B. [HSSP ENTRY / PDB]

ModBase

Q8CXC9.

Protein family/group databases

MEROPS

A25.001; -.

Enzyme and pathway databases

BioCyc

OIHE221109:OB1975-MON; -.

Ontologies

GO:0004222;	Molecular function: metalloendopeptidase activity (inferred from electronic annotation from HAMAP).
GO:0006508;	Biological process: proteolysis (inferred from electronic annotation from HAMAP).
GO:0009847;	Biological process: spore germination (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00626; -; 1.
PBIL [Tree]

InterPro

IPR005080; Peptidase_A25.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.10100; Peptidase_A25; 1.

Pfam

PF03418; Peptidase_A25; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF019549; Peptidase_A25; 1.

ProDom

PD041835; Peptidase_A25; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR01441; GPR; 1.

Genome annotation databases

1018452; -.

BA000028_GR; OB1975.

oih:OB1975; -.

fig|221109.1.peg.1975; -.

Phylogenomic databases

HOGENOM

Q8CXC9; -.

Genome annotation databases

CMR

Q8CXC9; OB1975.

Other

ProtoNet

Q8CXC9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Hydrolase; Protease; Zymogen.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`PROPEP`	`1 13`	`13`	`By similarity.`	`PRO_0000026878`
`CHAIN`	`14 367`	`354`	`Germination protease.`	`PRO_0000026879`

Sequence information

Length: 367 AA [This is the length of the unprocessed precursor]

Molecular weight: 40467 Da [This is the MW of the unprocessed precursor]

CRC64: 3F4985F73F2CE5C7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEEQQIPFQV RTDLAIEAKD MYTESKPEET NDKEIKGVTF KERSVKDIKV SYVDIDEEGE 

        70         80         90        100        110        120 
KLLGKKPGSY VTIYADGVKK QDTDRQGQAA QVLAKELEDL MRKNNVTKES TCLVVGLGNW 

       130        140        150        160        170        180 
NVTPDALGPM TVEKVLVTSH LFRLQYETVA QGYRDVAAVT PGVMGVTGIE TSDIIFGIVE 

       190        200        210        220        230        240 
KYKPDLVIAV DALASRSINR VNETIQLSDT GIHPGSGVGN KRKEISKKTL GIPVIAIGVP 

       250        260        270        280        290        300 
TVVDAVTITS DTIDYVLKHF GREWKEKDDP SKSLTPAGMS FGNRKLTDED LPNMEKRKTV 

       310        320        330        340        350        360 
LGIVGELSDE EKRKLITEVL TPLGHNLMVT PKEVDGFMID MAEVLANGIN AALHEKVDVD 


NFANYSR

Q8CXC9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools