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UniProtKB/Swiss-Prot entry Q8B6J5

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_RABVH
Primary accession number Q8B6J5
Secondary accession numbers None
Integrated into Swiss-Prot on July 10, 2007
Sequence was last modified on March 1, 2003 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 23)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Rabies virus (strain HEP-Flury) (RABV) [TaxID: 11296] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Lyssavirus.
Virus host Mammalia [TaxID: 40674]
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/S0166-0934(02)00249-5; PubMed=12505638 [NCBI, ExPASy, EBI, Israel, Japan]
Inoue K., Shoji Y., Kurane I., Iijima T., Sakai T., Morimoto K.;
"An improved method for recovering rabies virus from cloned cDNA.";
J. Virol. Methods 107:229-236(2003).
[2]
 INTERACTION WITH PROTEIN P.
STRAIN=CVS;
PubMed=9499045 [NCBI, ExPASy, EBI, Israel, Japan]
Chenik M., Schnell M., Conzelmann K.K., Blondel D.;
"Mapping the interacting domains between the rabies virus polymerase and phosphoprotein.";
J. Virol. 72:1925-1930(1998).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein to form the functional polymerase (By similarity).
  • SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the rhabdoviruses protein L family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB085828; BAC53869.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
3D structure databases
ModBase Q8B6J5.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037546; RNA_pol_RhabdoV_sub; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
Other
ProtoNet Q8B6J5.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2127  2127     Large structural protein. PRO_0000294421
DOMAIN   611    799  189     RdRp catalytic. 
REGION   1562   2127  566     Interaction with P protein. 
Sequence information
Length: 2127 AA [This is the length of the unprocessed precursor] Molecular weight: 242955 Da [This is the MW of the unprocessed precursor] CRC64: E9B31C8642375518 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLDPGEVYDD PIDPIESEAE PRGTPTVPNI LRNSDYNLNS PLIEDSAKLM LEWLKTGNRP 

        70         80         90        100        110        120 
YRMTLTDNCS RSYKDLKDYF KKVDLGSLKV GGTAAQSMIS LWLYGAHSES NRSRRCITDL 

       130        140        150        160        170        180 
AHFYSKSSPI EKLLNCTLGN RGLRIPPEGV LSCLERVDYD KAFGRYLANT YSSYLFFHVI 

       190        200        210        220        230        240 
TLYMNALDWE EEKTILALWK DLTSVDTGKD LVKFKDQIWG LLIVTKDFVY SQSSNCLFDR 

       250        260        270        280        290        300 
NYTLMLKDLF LSRFNSLMIL LSPPEPRYSD DLISQLCQLY IAGDQVLSLC GNSGYEVIKI 

       310        320        330        340        350        360 
LEPYVVNSLV QRAEKFRPLI HSLGDFPMFI KDKVNQLEGT FGPSAKRFFR VLDQFDNIHD 

       370        380        390        400        410        420 
LVFVYGCYRH WGHPYIDYRK GLLKLYDQVH IKKVIDKSYQ ECLASDLARR ILRWGFDKYS 

       430        440        450        460        470        480 
KWYLDSRFLA RDHPLAPYIK TQTWPPKHIV DLVGDTWHKL PITQIFEIPE SMDPSEILDD 

       490        500        510        520        530        540 
KSHSFTRTRL ASWLSENRGG PVPSEKVIIT ALSQPPVNPR EFLKSIDLGG LPDDDLIIGL 

       550        560        570        580        590        600 
RPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 

       610        620        630        640        650        660 
TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TEDVFSVLDQ VFGLKRVFSR THEFFQKSWI 

       670        680        690        700        710        720 
YYSDRSDLIG LREDQIYCLD MSNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 

       730        740        750        760        770        780 
RTKILAQGDN QVLCPTYMLS PGLSQEGLLY ELESISRNAL SIYRAIEEGA SKLGLIIKKE 

       790        800        810        820        830        840 
ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 

       850        860        870        880        890        900 
SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAE GESFLLAMSR IIYLDPSLGG 

       910        920        930        940        950        960 
VSGMSLGRFH IRQFSDPVSE GLSFWREIWL SSHESWIHAL CQEAGNPDLG ERTLESFTRL 

       970        980        990       1000       1010       1020 
LEDPTTLNIK GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSV 

      1030       1040       1050       1060       1070       1080 
EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRKS LSRTLEESFY NSEIHGINRM 

      1090       1100       1110       1120       1130       1140 
TQTPQRVGRV WPCSSERADL LREISWGRKV VGTTVPHPSE MLGLLPKSSI SCTCGATGGG 

      1150       1160       1170       1180       1190       1200 
NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RAISLKESIN 

      1210       1220       1230       1240       1250       1260 
WFINRNSNLA QTLIRNIMSL TGPDFSLEEA PVFKRTGSAL HRFKSARYSE GGYSSVCPNL 

      1270       1280       1290       1300       1310       1320 
LSHISVSTDT MSDLTQDGKN YDFMFQPLML YAQTWTSELV QRDTRLRDST FHWHLRCNRC 

      1330       1340       1350       1360       1370       1380 
VRPIEDITLE TSQIFEFPDV SKRISRMVSG AVPHFQKLPD IRLRPGDFES LSGREKSRHI 

      1390       1400       1410       1420       1430       1440 
GSAQGLLYSI LVAIHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGILIGS SICFLTRMTN 

      1450       1460       1470       1480       1490       1500 
INIKRPLELI SGVISYILLR LDNHPSLYIM LREPSLRGEI FSIPQKIPAA YPTTMKEGNR 

      1510       1520       1530       1540       1550       1560 
SILCYLQHVL RYEREVITAS PENDWLWIFS DFRSAKMTYL TLITYQSHLL LQRVERNLSK 

      1570       1580       1590       1600       1610       1620 
SMRATLQQMG SLMRQVLGGH GEDTLESDDD IQRLLKDSLR RTRWVDQEVR HAARTMSGDY 

      1630       1640       1650       1660       1670       1680 
SPNKRVSRKA GCSEWVCSAQ QVAVSTSANP APVSELDIRA LSKRFQNPLI SGLRVVQWAT 

      1690       1700       1710       1720       1730       1740 
GAHYKLKPIL DDLNVFPSLC LVVGDGSGGI SRAVLNMFPD SKLVFNSLLE VNDLMASGTR 

      1750       1760       1770       1780       1790       1800 
PLPPSAIMSG GDDIISRVID FDSIWEKPSD LRNLATWRYF QSVQKQVNMS YDLIICDAEV 

      1810       1820       1830       1840       1850       1860 
TDIASINRIT LLMSDFALSI DGPLYLVFKT YGTMLVNPDY KAIQHLSRAF PSVTGFITQV 

      1870       1880       1890       1900       1910       1920 
TSSFSSELYL RFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPKSEMQRA RSLNYQDLVR 

      1930       1940       1950       1960       1970       1980 
GFPEEIISNP YNEMIITLID SDVESFLVHK MVDDLELQRG TLSKVAIIIS IMIVFSNRVF 

      1990       2000       2010       2020       2030       2040 
NISKPLTDPL FYPPSDPKIL RHFNICCSTM MYLSTALGDV PSFARLHDLY NRPITYYFRK 

      2050       2060       2070       2080       2090       2100 
QVIRGNIYLS WSWSDDTPVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG RIEDLSGEVE 

      2110       2120 
RHLHGYNRWI TLEDIRSRSS LLDYSCL
Q8B6J5 in FASTA format

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