[1]	NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. DOI=10.1016/S0166-445X(03)00057-2; PubMed=12842592 [NCBI, ExPASy, EBI, Israel, Japan] Hegelund T., Celander M.C.; "Hepatic versus extrahepatic expression of CYP3A30 and CYP3A56 in adult killifish (Fundulus heteroclitus)."; Aquat. Toxicol. 64:277-291(2003).
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 312-435. TISSUE=Liver; DOI=10.1006/bbrc.1997.6956; PubMed=9240431 [NCBI, ExPASy, EBI, Israel, Japan] Celander M.C., Stegeman J.J.; "Isolation of a cytochrome P450 3A cDNA sequence (CYP3A30) from the marine teleost Fundulus heteroclitus and phylogenetic analyses of CYP3A genes."; Biochem. Biophys. Res. Commun. 236:306-312(1997).

Comments

FUNCTION: Putative steroid 6-beta-hydroxylase.
CATALYTIC ACTIVITY: RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein (Potential). Microsome membrane; Peripheral membrane protein (Potential).
TISSUE SPECIFICITY: Highly expressed in liver and intestine. Moderate expression in gill and spleen. Low expression in kidney, brain and heart.
SIMILARITY: Belongs to the cytochrome P450 family.
CAUTION: Due to a recent gene duplication event, CYP3A30 and CYP3A56 are very similar. Because of this it was not possible to distinguish between the two genes when measuring the tissue expression.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY143428; AAN38837.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P14779; 1JPZ. [HSSP ENTRY / PDB]

ModBase

Q8AXY5.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005792;	Cellular component: microsome (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0020037;	Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0050381;	Molecular function: unspecific monooxygenase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR008072; Cyt_P450_E_CYP3A.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR01689; EP450IICYP3A.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Phylogenomic databases

HOVERGEN

Q8AXY5; -.

Other

ProtoNet

Q8AXY5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 496`	`496`	`Cytochrome P450 3A56.`	`PRO_0000051810`
`METAL`	`441 441`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 496 AA [This is the length of the unprocessed precursor]

Molecular weight: 57054 Da [This is the MW of the unprocessed precursor]

CRC64: BAF945B260CA0220 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGYFYLTAET WTLLVAFVTL LLIYAYWPYG TFKRLGISGP KPVPFFGTML HYRRGFFTFD 

        70         80         90        100        110        120 
EECKKKYGKV WGIYDGRQPV LCVTDPEIIK AVLVKECLSF FTNRRNFHLN GPLYDALSVA 

       130        140        150        160        170        180 
EDDQWKRIRS VLSPSFTSGR LKEMFEIMKN HSANLIRSMK KKADKDEPLD LKEFFGSYSM 

       190        200        210        220        230        240 
DVVTSTAFSV DIDSLNNPSD PFVTNIKKML KFDFLNPLFL AVAFFPFLGP ILEKFELSFF 

       250        260        270        280        290        300 
PKSVTDFFYA SLEKIKSNRE ASQQKSRVDF LQLMIDSQKN SGAQQDKSLT DHEILSQSMI 

       310        320        330        340        350        360 
FIFAGYETSS SSLTFLAYNL ATNPEVMKKL QEEIDATFPN KAPVHYQPLM EMEYLDCVIN 

       370        380        390        400        410        420 
ESLRLFPIAA RLERVAKAAV EINGIVIPKD MVVMIPTWPL HRDPEIWPEP EAFKPERFSK 

       430        440        450        460        470        480 
KNKDNIDPYI YMPFGSGPRN CIGMRFALVL IKLAVVEILQ QYSFSVCKET EVPFEMDIQG 

       490 
LLAPKRPIQL KLVPRS

Q8AXY5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools