NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nbt1010; PubMed=15378067 [NCBI, ExPASy, EBI, Israel, Japan]
Hong S.H., Kim J.S., Lee S.Y., In Y.H., Choi S.S., Rih J.-K., Kim C.H., Jeong H., Hur C.G., Kim J.J.;
"The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens.";
Nat. Biotechnol. 22:1275-1281(2004).

Comments

FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016827; AAU38888.1; ALT_INIT; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_089473.1; -.

3D structure databases

SMR

Q65Q72; 38-829.

ModBase

Q65Q72.

Ontologies

GO:0042597;	Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from InterPro).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0030151;	Molecular function: molybdenum ion binding (inferred from electronic annotation from InterPro).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR010051; NO3_reductase_lsu_periplasm.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01706; NAPA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

3075593; -.

AE016827_GR; MS2281.

msu:MS2281; -.

fig|221988.1.peg.2146; -.

Phylogenomic databases

HOGENOM

Q65Q72; -.

Genome annotation databases

CMR

Q65Q72; MS2281.

Other

ProtoNet

Q65Q72.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 32`	`32`	`Tat-type signal (Potential).`
`CHAIN`	`33 830`	`798`	`Periplasmic nitrate reductase.`	`PRO_0000045991`
`METAL`	`46 46`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`49 49`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`53 53`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`81 81`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 830 AA [This is the length of the unprocessed precursor]

Molecular weight: 93765 Da [This is the MW of the unprocessed precursor]

CRC64: AF0EDF9F2472B59E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MELNRRDFMK ANAAVAAAAA AGITIPVKNV HAADDDMGIR WDKAPCRYCG TGCSVLVGTK 

        70         80         90        100        110        120 
DGRVVATQGD PDAEVNRGLN CIKGYFLSKI MYGADRVQTP LLRMKDGKFH KEGDFTPVSW 

       130        140        150        160        170        180 
DQAFTVMADK IKAILKEKKD PNAIGMFSSG QTTIFEGYAK VKLWKAGLRS NTIDPNARHC 

       190        200        210        220        230        240 
MASAAVAFLR TFGMDEPMGC YNDIEKTDAF VLWGSNMAEM HPILWSRISD RRLSSDKVKV 

       250        260        270        280        290        300 
VVMSTFEHRS FELADTPIIF KPHSDLAILN YIANYIIQND KVNWDFVNKH TKFKRGETDI 

       310        320        330        340        350        360 
GYGLRPEHPL EVAAKNRKTA GKMYDSDFEE FKKIVAPYTL EEAHRISGVP KDQLETLAKM 

       370        380        390        400        410        420 
YADPQQNLVS FWTMGFNQHT RGVWVNHMVY NVHLLTGKIS KPGCGPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFVHRLP ADMVVTNPKH VEIAENIWKL PKGTISNKPG FPAVQQSRAL KDGKLNFLWQ 

       490        500        510        520        530        540 
LCTNNMQGGP NINEEIFPGW RNPDNLIVVS DPYPSASAVA ADLILPTCMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQFWRQQ VKGPGQSRSD LWQIVEFSKY FKTEEVWSEE LLAQMPEYRG KTLYEVLYLN 

       610        620        630        640        650        660 
GEVNKFQTPT NVPGYINDEA EDFGFYLQKG LFEEYASFGR GHGHDLADFD TYHQVRGLRW 

       670        680        690        700        710        720 
PVVDGKETLW RYREGYDPYV KAGEEVSFYG YPDKKAIILG VPYEAPAESP DEEYDLWLCT 

       730        740        750        760        770        780 
GRVLEHWHTG TMTRRVPELH KAFPNNLCWM HPTDAKKRGL RHGDKVKLIT RRGEMISHLD 

       790        800        810        820        830 
TRGRNKCPEG LIYTTFFDAG QLANKLTLDA TDPISGETDY KKCAVKVVKA

Q65Q72 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools