Microtubule-associated protein 1 light chain 3 beta
MAP1A/1B light chain 3 B
MAP1A/MAP1B LC3 B
MAP1 light chain 3-like protein 2
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B

Gene name

	Name:	Map1lc3b
	Synonyms:	Map1alc3, Map1lc3

From

Rattus norvegicus (Rat)

[TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

Protein existence

1: Evidence at protein level;

References

[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). TISSUE=Brain; PubMed=7908909 [NCBI, ExPASy, EBI, Israel, Japan] Mann S.S., Hammarback J.A.; "Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B."; J. Biol. Chem. 269:11492-11497(1994).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). STRAIN=Sprague-Dawley; TISSUE=Brain; Dang Y., Yu L., Wu J., Pei Y.; "Cloning and characterization of rat lc3 orthologs."; Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). STRAIN=Sprague-Dawley; Zhou G., Dai F., Yu L.; "Rattus norvegicus microtubule-associated protein 1 light chain 3 (Map1lc3)."; Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). TISSUE=Heart, and Pituitary; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[5]	PROTEIN SEQUENCE OF 31-37, AND MASS SPECTROMETRY. STRAIN=Sprague-Dawley; TISSUE=Brain; Lubec G., Kang S.U.; Submitted (JUL-2007) to UniProtKB.
[6]	SUBCELLULAR LOCATION, CLEAVAGE, AND MUTAGENESIS OF GLY-120. DOI=10.1093/emboj/19.21.5720; PubMed=11060023 [NCBI, ExPASy, EBI, Israel, Japan] Kabeya Y., Mizushima N., Ueno T., Yamamoto A., Kirisako T., Noda T., Kominami E., Ohsumi Y., Yoshimori T.; "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing."; EMBO J. 19:5720-5728(2000).
[7]	REVIEW. DOI=10.1016/j.biocel.2004.05.009; PubMed=15325588 [NCBI, ExPASy, EBI, Israel, Japan] Tanida I., Ueno T., Kominami E.; "LC3 conjugation system in mammalian autophagy."; Int. J. Biochem. Cell Biol. 36:2503-2518(2004).

Comments

FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).
SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.
SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID Q62625-1

This is the isoform sequence displayed in this entry.

Name 2

Isoform ID Q62625-2

Features which should be applied to build the isoform sequence: VSP_012092.
TISSUE SPECIFICITY: Abundant only in neurons. Detected in testes.
PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
SIMILARITY: Belongs to the MAP1 LC3 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U05784; AAA20645.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY206669; AAP42561.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY392036; AAQ94605.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC058144; AAH58144.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC083556; AAH83556.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A53624; A53624.

NP_074058.2; -.

3D structure databases

PDB

1UGM; X-ray; 2.05 A; A=1-120.	[ExPASy / RCSB / EBI]
2Z0D; X-ray; 1.90 A; B=1-120.	[ExPASy / RCSB / EBI]
2Z0E; X-ray; 1.90 A; B=1-124.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1UGM; -.
2Z0D; -.
2Z0E; -.

ModBase

Q62625.

Organism-specific databases

RGD

621315; Map1lc3b.

Gene expression databases

ArrayExpress

Q62625; -.

GermOnline

ENSRNOG00000038106; Rattus norvegicus.

Ontologies

GO:0000421;	Cellular component: autophagic vacuole membrane (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR004241; MAP1_LC3.
Graphical view of domain structure.

PANTHER

PTHR10969; MAP1_LC3; 1.

Pfam

PF02991; MAP1_LC3; 1.
Pfam graphical view of domain structure.

BLOCKS

Q62625.

Genome annotation databases

Ensembl

ENSRNOG00000038106; Rattus norvegicus. [Contig view]

GeneID

64862; -.

KEGG

rno:64862; -.

Phylogenomic databases

HOVERGEN

Q62625; -.

Other

ProtoNet

Q62625.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Alternative splicing; Autophagy; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Lipoprotein; Membrane; Microtubule; Ubl conjugation pathway.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 120`	`119`	`Microtubule-associated proteins 1A/1B light chain 3B.`	`PRO_0000017202`
`PROPEP`	`121 142`	`22`	`Removed in mature form (Probable).`	`PRO_0000017203`
`LIPID`	`120 120`		`Phosphatidylethanolamine amidated glycine (By similarity).`
`VAR_SEQ`	`126 142`		`Missing (in isoform 2).`	`VSP_012092`
`MUTAGEN`	`120 120`		`G->A: No processing of precursor.`
`HELIX`	`7 10`	`4`
`HELIX`	`13 26`	`14`
`STRAND`	`30 37`	`8`
`STRAND`	`50 55`	`6`
`HELIX`	`60 70`	`11`
`STRAND`	`80 83`	`4`
`STRAND`	`91 94`	`4`
`HELIX`	`95 102`	`8`
`STRAND`	`109 115`	`7`

Sequence information

Length: 142 AA [This is the length of the unprocessed precursor]

Molecular weight: 16394 Da [This is the MW of the unprocessed precursor]

CRC64: D07E9D063C125E32 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 

        70         80         90        100        110        120 
SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SERDEDGFLY MVYASQETFG 

       130        140 
TALAVTYMSA LKATATGREP CL

Q62625 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools