NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.00810-06; PubMed=16980466 [NCBI, ExPASy, EBI, Israel, Japan]
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes.";
J. Bacteriol. 188:7922-7931(2006).

[2]

PROTEIN SEQUENCE OF 2-35.
PubMed=8620891 [NCBI, ExPASy, EBI, Israel, Japan]
Bock A.-K., Kunow J., Glasemacher J., Schoenheit P.;
"Catalytic properties, molecular composition and sequence alignments of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina barkeri (strain Fusaro).";
Eur. J. Biochem. 237:35-44(1996).

Comments

CATALYTIC ACTIVITY: Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO₂ + 2 reduced ferredoxin + 2 H⁺.
SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma chain.
MISCELLANEOUS: It also catalyzes the oxidation of 2-oxobutyrate.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000099; AAZ69967.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S65417; S65417.

RefSeq

YP_304547.1; -.

3D structure databases

ModBase

P80522.

Enzyme and pathway databases

BioCyc

MBAR269797:MBAR_A0999-MON; -.

Ontologies

GO:0019164;	Molecular function: pyruvate synthase activity (inferred from electronic annotation from EC).
GO:0030976;	Molecular function: thiamin pyrophosphate binding (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011766; TPP_enzyme_bd_C.
Graphical view of domain structure.

Pfam

PF02775; TPP_enzyme_C; 1.
Pfam graphical view of domain structure.

ProtoNet

P80522.

Genome annotation databases

GeneID

3626882; -.

GenomeReviews

CP000099_GR; Mbar_A0999.

KEGG

mba:Mbar_A0999; -.

NMPDR

fig|269797.3.peg.1010; -.

Phylogenomic databases

HOGENOM

P80522; -.

Genome annotation databases

CMR

P80522; Mbar_A0999.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Direct protein sequencing; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 296`	`295`	`Pyruvate synthase subunit porB.`	`PRO_0000097126`

Sequence information

Length: 296 AA [This is the length of the unprocessed precursor]

Molecular weight: 32056 Da [This is the MW of the unprocessed precursor]

CRC64: C040A6216787AC14 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSKTAPKTYI TSGHSGCAGC CDAFAAKFTL MGAGPNTIVI NPTGCLEVMS TPFPYSSWQV 

        70         80         90        100        110        120 
PWIHSLFENA GAVASGVEAA LKALGKKDDV KVVSIGGDGS TMDIGLGALS GAFERGHDFT 

       130        140        150        160        170        180 
YVCMDNEAYM NTGVQRSSGT PFDASTTTTP AGKVSFGNPR PKKNMPAIMA AHGSPYVATT 

       190        200        210        220        230        240 
SIGFPRDMIR KVKKATEIVG PTYIHAQAPC PTGWGFDTSK TLEIAKLAVE TCLWPMYEME 

       250        260        270        280        290 
NGEITQVRKV KNPRPVEEYL RAQKRFKHLF TMEGGEEEIK KIQAIADWNI KHFELQ

P80522 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools