NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Isolate 19;
DOI=10.1016/0378-1119(95)00169-7; PubMed=7789806 [NCBI, ExPASy, EBI, Israel, Japan]
Williams R.S.B., Davis M.A., Howlett B.J.;
"The nitrate and nitrite reductase-encoding genes of Leptosphaeria maculans are closely linked and transcribed in the same direction.";
Gene 158:153-154(1995).

Comments

CATALYTIC ACTIVITY: Ammonium hydroxide + 3 NAD(P)⁺ + H₂O = nitrite + 3 NAD(P)H.
COFACTOR: Binds 1 siroheme per subunit (By similarity).
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
COFACTOR: FAD (By similarity).
PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
SUBUNIT: Homodimer (By similarity).

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U18793; AAA82739.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

PC4032; PC4032.

3D structure databases

HSSP

Q52437; 1D7Y. [HSSP ENTRY / PDB]

ModBase

P43504.

Ontologies

GO:0051537;	Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0050660;	Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008942;	Molecular function: nitrite reductase [NAD(P)H] activity (inferred from electronic annotation from EC).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR006066; Nir_Si_BS.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.

Pfam

PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
Pfam graphical view of domain structure.

PROSITE

PS00365; NIR_SIR; PARTIAL.

Other

ProtoNet

P43504.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; FAD; Flavoprotein; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Nitrate assimilation; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >185`	`>185`	`Nitrite reductase [NAD(P)H].`	`PRO_0000199959`
`NP_BIND`	`72 107`	`36`	`FAD (Potential).`
`NON_TER`	`185 185`

Sequence information

Length: 185 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 20756 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 19D67F97D386E7F9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAATLPLLTQ GIEPVSGESY SPPGERHVQA TWGSKDGNIS NTEWNDPEAN RLPEKVAELE 

        70         80         90        100        110        120 
KKGELNNSHP RRRVVVVGLG MVGVAFIEKL MKYDIKRREY DIIVIGEEPH LAYNRVGLTS 

       130        140        150        160        170        180 
FFQHRQVENL YLNPQEWYSS MPEDSLHYHL NTLVTEIDSE NKTVKTSSGQ AVSYDILVLA 


TGSSS

P43504 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools