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UniProtKB/Swiss-Prot entry P32559


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MSS1_YEAST
Primary accession number P32559
Secondary accession number Q0PHA7
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1996 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 67)
Name and origin of the protein
Protein name tRNA modification GTPase MSS1, mitochondrial [Precursor]
Synonyms None
Gene name
Name: MSS1
Synonyms: PET53
OrderedLocusNames: YMR023C
ORFNames: YM9711.13C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1006/jmbi.1993.1371; PubMed=8392589 [NCBI, ExPASy, EBI, Israel, Japan]
Decoster E., Vassal A., Faye G.;
"MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase.";
J. Mol. Biol. 232:79-88(1993).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Li X., Wang X., Guan M.-X.;
"MSS1, a mitochondrial GTP-binding protein involved in mitochondrial tRNA modification.";
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169872 [NCBI, ExPASy, EBI, Israel, Japan]
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
Nature 387:90-93(1997).
[4]
INTERACTION WITH MTO1.
DOI=10.1074/jbc.273.43.27945; PubMed=9774408 [NCBI, ExPASy, EBI, Israel, Japan]
Colby G., Wu M., Tzagoloff A.;
"MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae.";
J. Biol. Chem. 273:27945-27952(1998).
[5]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02026; PubMed=14562095 [NCBI, ExPASy, EBI, Israel, Japan]
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.;
"Global analysis of protein localization in budding yeast.";
Nature 425:686-691(2003).
[6]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[7]
FUNCTION.
DOI=10.1074/jbc.M409306200; PubMed=15509579 [NCBI, ExPASy, EBI, Israel, Japan]
Umeda N., Suzuki T., Yukawa M., Ohya Y., Shindo H., Watanabe K., Suzuki T.;
"Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases.";
J. Biol. Chem. 280:1613-1624(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X69481; CAA49238.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ834923; ABG77646.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z49211; CAA89126.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S54025; S54025.
RefSeq NP_013736.1; -.
3D structure databases
ModBase P32559.
Protein-protein interaction databases
DIP DIP:5882N; -.
IntAct P32559; -.
Organism-specific databases
CYGD YMR023c; -.
SGD S000004625; MSS1.
Yeast-GFP YMR023C.
Gene expression databases
GermOnline YMR023C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005743; Cellular component: mitochondrial inner membrane (inferred from direct assay from SGD).
GO:0006412; Biological process: translation (inferred from mutant phenotype from SGD).
GO:0006400; Biological process: tRNA modification (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR005289; GTP_bd.
IPR002917; MMR_HSR1_GTP_bd.
IPR001806; Ras_trnsfrmng.
IPR005225; Small_GTP_bd.
IPR004520; ThdF.
Graphical view of domain structure.
Pfam PF01926; MMR_HSR1; 1.
Pfam graphical view of domain structure.
PRINTS PR00449; RASTRNSFRMNG.
TIGRFAMs TIGR00650; MG442; 1.
TIGR00450; mnmE_trmE_thdF; 1.
TIGR00231; small_GTP; 1.
BLOCKS P32559.
Genome annotation databases
Ensembl YMR023C; Saccharomyces cerevisiae. [Contig view]
GeneID 855037; -.
GenomeReviews Z71257_GR; YMR023C.
KEGG sce:YMR023C; -.
NMPDR fig|4932.3.peg.4782; -.
Phylogenomic databases
HOGENOM P32559; -.
Other
LinkHub P32559; -.
ProtoNet P32559.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; GTP-binding; Mitochondrion; Nucleotide-binding; Transit peptide; tRNA processing.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    19  19     Mitochondrion (Potential). 
CHAIN   20   526  507     tRNA modification GTPase MSS1, mitochondrial. PRO_0000035781
NP_BIND   281   288  8     GTP (Potential). 
NP_BIND   328   332  5     GTP (Potential). 
NP_BIND   394   397  4     GTP (Potential). 
CONFLICT   53    53        A -> R (in Ref. 1; CAA49238). 
Sequence information
Length: 526 AA [This is the length of the unprocessed precursor] Molecular weight: 58352 Da [This is the MW of the unprocessed precursor] CRC64: 8740A374CA1E637D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNSASFLQSR LISRSFLVRR SLKRYSGLAK PYTFQQPTIY ALSTPANQTS AIAIIRISGT 

        70         80         90        100        110        120 
HAKYIYNRLV DSSTVPPIRK AILRNIYSPS SCSVKPHDQK ESKILLDTSL LLYFQAPYSF 

       130        140        150        160        170        180 
TGEDVLELHV HGGKAVVNSI LKAIGSLHDR SSGKDIRFAL PGDFSRRAFQ NGKFDLTQLE 

       190        200        210        220        230        240 
GIKDLIDSET ESQRRSALSS FNGDNKILFE NWRETIIENM AQLTAIIDFA DDNSQEIQNT 

       250        260        270        280        290        300 
DEIFHNVEKN IICLRDQIVT FMQKVEKSTI LQNGIKLVLL GAPNVGKSSL VNSLTNDDIS 

       310        320        330        340        350        360 
IVSDIPGTTR DSIDAMINVN GYKVIICDTA GIREKSSDKI EMLGIDRAKK KSVQSDLCLF 

       370        380        390        400        410        420 
IVDPTDLSKL LPEDILAHLS SKTFGNKRII IVVNKSDLVS DDEMTKVLNK LQTRLGSKYP 

       430        440        450        460        470        480 
ILSVSCKTKE GIESLISTLT SNFESLSQSS ADASPVIVSK RVSEILKNDV LYGLEEFFKS 

       490        500        510        520 
KDFHNDIVLA TENLRYASDG IAKITGQAIG IEEILDSVFS KFCIGK 

P32559 in FASTA format

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