[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE. PubMed=3997817 [NCBI, ExPASy, EBI, Israel, Japan] Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K., Bonam D., Baldwin T.O.; "Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase."; J. Biol. Chem. 260:6139-6146(1985).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=CTP5; DOI=10.1007/BF00280295; PubMed=1685011 [NCBI, ExPASy, EBI, Israel, Japan] Escher A., O'Kane D.J., Szalay A.A.; "The beta subunit polypeptide of Vibrio harveyi luciferase determines light emission at 42 degrees C."; Mol. Gen. Genet. 230:385-393(1991).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28 AND 74-133. STRAIN=ATCC 33843 / 392 / MAV; PubMed=6571986 [NCBI, ExPASy, EBI, Israel, Japan] Cohn D.H., Ogden R.C., Abelson J.N., Baldwin T.O., Nealson K.H., Simon M.I., Mileham A.J.; "Cloning of the Vibrio harveyi luciferase genes: use of a synthetic oligonucleotide probe."; Proc. Natl. Acad. Sci. U.S.A. 80:120-123(1983).
[4]	X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). DOI=10.1021/bi00020a002; PubMed=7756289 [NCBI, ExPASy, EBI, Israel, Japan] Fisher A.J., Raushel F.M., Baldwin T.O., Rayment I.; "Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4-A resolution."; Biochemistry 34:6581-6586(1995).
[5]	X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS). DOI=10.1074/jbc.271.36.21956; PubMed=8703001 [NCBI, ExPASy, EBI, Israel, Japan] Fisher A.J., Thompson T.B., Thoden J.B., Baldwin T.O., Rayment I.; "The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions."; J. Biol. Chem. 271:21956-21968(1996).

Comments

FUNCTION: Light-emitting reaction in luminous bacteria.
CATALYTIC ACTIVITY: RCHO + reduced FMN + O₂ = RCOOH + FMN + H₂O + light.
SUBUNIT: Heterodimer of an alpha and a beta chain.
MISCELLANEOUS: The synthesis of this protein is regulated by a complex control mechanism that has been termed autoinduction. In fully induced cells luciferase comprises up to 5% of the soluble protein.
SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M10961; AAA88685.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X58791; CAA41597.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
V01422; CAA24692.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
V01423; CAA24693.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A22613; A22613.

3D structure databases

PDB

1BRL; X-ray; 2.40 A; A/C=1-355.	[ExPASy / RCSB / EBI]
1LUC; X-ray; 1.50 A; A=1-355.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1BRL; -.
1LUC; -.

ModBase

P07740.

Ontologies

GO:0047646;	Molecular function: alkanal monooxygenase (FMN-linked) activity (inferred from electronic annotation from EC).
GO:0008218;	Biological process: bioluminescence (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011251; Luciferase-like_bac.
IPR002103; Luciferase_bac.
IPR016048; Luciferase_mOase.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.30; Luciferase_like; 1.

Pfam

PF00296; Bac_luciferase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00089; LUCIFERASE.

PROSITE

PS00494; BACTERIAL_LUCIFERASE; 1.

Other

P07740; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Direct protein sequencing; Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase; Photoprotein.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 355`	`355`	`Alkanal monooxygenase alpha chain.`	`PRO_0000220172`
`CONFLICT`	`17 17`		`Q -> E (in Ref. 1; AA sequence).`
`CONFLICT`	`68 68`		`T -> K (in Ref. 2; CAA41597).`
`CONFLICT`	`169 169`		`P -> A (in Ref. 1; AA sequence).`
`CONFLICT`	`174 174`		`A -> P (in Ref. 1; AA sequence).`
`CONFLICT`	`204 205`		`QL -> HV (in Ref. 2; CAA41597).`
`CONFLICT`	`238 238`		`R -> K (in Ref. 2; CAA41597).`
`CONFLICT`	`321 321`		`D -> N (in Ref. 2; CAA41597).`
`STRAND`	`2 7`	`6`
`HELIX`	`17 33`	`17`
`STRAND`	`39 42`	`4`
`HELIX`	`55 64`	`10`
`STRAND`	`71 78`	`8`
`HELIX`	`83 97`	`15`
`STRAND`	`101 106`	`6`
`HELIX`	`111 117`	`7`
`HELIX`	`124 141`	`18`
`STRAND`	`142 144`	`3`
`STRAND`	`148 150`	`3`
`STRAND`	`170 173`	`4`
`HELIX`	`177 185`	`9`
`STRAND`	`190 192`	`3`
`STRAND`	`194 196`	`3`
`HELIX`	`198 214`	`17`
`TURN`	`219 221`	`3`
`STRAND`	`225 229`	`5`
`HELIX`	`236 256`	`21`
`STRAND`	`262 265`	`4`
`HELIX`	`295 298`	`4`
`STRAND`	`299 303`	`5`
`HELIX`	`304 318`	`15`
`STRAND`	`322 326`	`5`
`HELIX`	`328 330`	`3`
`HELIX`	`333 346`	`14`

Sequence information

Length: 355 AA [This is the length of the unprocessed precursor]

Molecular weight: 40153 Da [This is the MW of the unprocessed precursor]

CRC64: F915E6BBE41ACEEE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKFGNFLLTY QPPELSQTEV MKRLVNLGKA SEGCGFDTVW LLEHHFTEFG LLGNPYVAAA 

        70         80         90        100        110        120 
HLLGATETLN VGTAAIVLPT AHPVRQAEDV NLLDQMSKGR FRFGICRGLY DKDFRVFGTD 

       130        140        150        160        170        180 
MDNSRALMDC WYDLMKEGFN EGYIAADNEH IKFPKIQLNP SAYTQGGAPV YVVAESASTT 

       190        200        210        220        230        240 
EWAAERGLPM ILSWIINTHE KKAQLDLYNE VATEHGYDVT KIDHCLSYIT SVDHDSNRAK 

       250        260        270        280        290        300 
DICRNFLGHW YDSYVNATKI FDDSDQTKGY DFNKGQWRDF VLKGHKDTNR RIDYSYEINP 

       310        320        330        340        350 
VGTPEECIAI IQQDIDATGI DNICCGFEAN GSEEEIIASM KLFQSDVMPY LKEKQ

P07740 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools