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UniProtKB/Swiss-Prot entry P00015


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CYC2_MOUSE
Primary accession number P00015
Secondary accession numbers None
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 83)
Name and origin of the protein
Protein name Cytochrome c, testis-specific
Synonyms None
Gene name
Name: Cyct
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2834389 [NCBI, ExPASy, EBI, Israel, Japan]
Virbasius J.V., Scarpulla R.C.;
"Structure and expression of rodent genes encoding the testis-specific cytochrome c. Differences in gene structure and evolution between somatic and testicular variants.";
J. Biol. Chem. 263:6791-6796(1988).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Testis;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
PRELIMINARY PROTEIN SEQUENCE OF 2-105.
STRAIN=BALB/c;
PubMed=240690 [NCBI, ExPASy, EBI, Israel, Japan]
Hennig B.;
"Change of cytochrome c structure during development of the mouse.";
Eur. J. Biochem. 55:167-183(1975).
Comments
  • FUNCTION: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
  • SUBCELLULAR LOCATION: Mitochondrion matrix.
  • TISSUE SPECIFICITY: This is one of two isocytochromes C found in the testis. The other is identical with the form found in other mouse tissues. These cytochromes are assumed to be located in the sperm.
  • PTM: Binds 1 heme group per subunit.
  • SIMILARITY: Belongs to the cytochrome c family.
  • WEB RESOURCE: Name=Protein Spotlight; Note=Life shuttle - Issue 76 of November 2006; URL="http://www.expasy.org/spotlight/back_issues/sptlt076.shtml";.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M20625; AAA37501.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X55771; CAA39293.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK005582; BAB24136.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK018833; BAB31455.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK018851; BAB31464.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B28160; CCMST.
RefSeq NP_034119.1; -.
UniGene Mm.4872
3D structure databases
PDB
2AIU; X-ray; 1.60 A; A=1-105.[ExPASy / RCSB / EBI]
PDBsum 2AIU; -.
ModBase P00015.
Organism-specific databases
MGI MGI:88579; Cyct.
Gene expression databases
ArrayExpress P00015; -.
CleanEx MM_CYCT; -.
GermOnline ENSMUSG00000056436; Mus musculus.
Ontologies
GO
GO:0020037; Molecular function: heme binding (inferred from direct assay from MGI).
GO:0006915; Biological process: apoptosis (inferred from direct assay from MGI).
GO:0042743; Biological process: hydrogen peroxide metabolic process (inferred from direct assay from MGI).
QuickGo view.
Family and domain databases
InterPro IPR009056; Cyt_c_monohaem.
IPR003088; Cyt_CI.
IPR002327; Cyt_CIAB.
Graphical view of domain structure.
Gene3D G3DSA:1.10.760.10; Cytochrome_c_R; 1.
PANTHER PTHR11961; Cyt_CIAB; 1.
Pfam PF00034; Cytochrom_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00604; CYTCHRMECIAB.
ProDom PD000375; Cyt_CIAB; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS51007; CYTC; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P00015.
Genome annotation databases
Ensembl ENSMUSG00000056436; Mus musculus. [Contig view]
GeneID 13067; -.
KEGG mmu:13067; -.
NMPDR fig|10090.3.peg.6178; -.
Phylogenomic databases
HOGENOM P00015; -.
HOVERGEN P00015; -.
Other
SOURCE Cyct; Mus musculus.
ProtoNet P00015.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; Direct protein sequencing; Electron transport; Heme; Iron; Metal-binding; Mitochondrion; Respiratory chain; Transport.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   105  104     Cytochrome c, testis-specific. PRO_0000108226
METAL   19    19        Iron (heme axial ligand). 
METAL   81    81        Iron (heme axial ligand). 
BINDING   15    15        Heme (covalent). 
BINDING   18    18        Heme (covalent). 
MOD_RES   2     2        N-acetylglycine. 
HELIX   4    14  11      
TURN   15    18  4      
STRAND   39    42  4      
HELIX   51    55  5      
HELIX   62    70  9      
HELIX   72    75  4      
HELIX   89   104  16      
Sequence information
Length: 105 AA [This is the length of the unprocessed precursor] Molecular weight: 11714 Da [This is the MW of the unprocessed precursor] CRC64: 00C275FC70B5AAF9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGDAEAGKKI FVQKCAQCHT VEKGGKHKTG PNLWGLFGRK TGQAPGFSYT DANKNKGVIW 

        70         80         90        100 
SEETLMEYLE NPKKYIPGTK MIFAGIKKKS EREDLIKYLK QATSS 

P00015 in FASTA format

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