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MONDAY AUGUST 31st
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|
8.00 -9.00
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Registration
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9.00 - 9.05
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OPENING REMARKS
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|
|
V.
Pallini, D. F.Hochstrasser
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9.05 - 9.15
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WELCOME ADDRESS
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|
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Professor
P.Tosi, President of Siena University
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OPENING LECTURE
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9.15 - 10.00
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GENOTYPES AND
PHENOTYPES
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|
|
J.
Klose (Berlin , Germany)
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PLENARY SESSION 1
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| SAMPLE PREPARATION
AND SOLUBILISATION
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| Chairpersons:
T. Rabilloud, B.Herbert
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10.00 - 10.20
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CAN
WE REALLY DO PROTEOMICS ?
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|
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T.
Rabilloud, M. Chevallet, S. Charmont,J. Lunardi (Grenoble, France)
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10.20 - 10.40
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STRATEGIES
FOR SAMPLE PREPARATION FOR TWO-
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| DIMENSIONAL
ELECTROPHORESIS
| |
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|
B.R.
Herbert, M.P. Molloy, T. Rabilloud, J-C. Sanchez, D.F. Hochstrasser,
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|
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A.A.
Gooley, K.L. Williams (Sydney, Australia; Grenoble, France; Geneva,
Switzerlamd)
|
| 10.40
- 11.00
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USING
LASER CAPTURE MICRODISSECTION TO SELECTIVELY
|
| OBTAIN DISTINCT
POPULATIONS OF CELLS FOR PROTEOMIC
| |
|
|
ANALYSIS
- PRELIMINARY FINDINGS
|
| R
E Banks, M J Dunn, M A Forbes, A Stanley, DPappin, M Gough, P Harnden,
| |
| P
J Selby (Leeds, Harefield, London;UK)
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11.00 - 11.30
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COFFEE BREAK
|
PLENARY SESSION 2
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| pH,
pI,pK AND RELEVANT GRADIENTS
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| Chairpersons:
A. Görg, B.Bjellqvist
|
11.30 - 11.50
|
RECENT DEVELOPMENTS
IN IPG-DALT: WIDE PH GRADIENTS UP
|
| TO
pH 12, LONGER SEPARATION DISTANCES AND
| |
| PROCEDURES
| |
| A.
Görg, C. Obermaier, G. Boguth, W. Weiss(Munich, Germany),
|
11.50 - 12.10
|
COMPARISON
OFTHE MOBILITIES OF PROTEINS ON AMPHOLYTE
|
| AND
IMMOBILISED pH GRADIENT BASED 2D GELS
| |
| S.
J. Fey, A. Nawrocki, M. R. Larsen, A. Podtelejnikov, O. N. Jensen, M.
| |
| Mann,
P. Roepstorff, A. Goerg, P. Mose Larsen (Odense, Denmark,Munich,
Germany.)
|
12.10 - 12.30
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CAPILLARY ZONE
ELECTROPHORESIS OF PEPTIDES AND
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| PROTEINS
IN ISOELECTRIC BUFFERS: THEORY AND
| |
|
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METHODOLOGY
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| P.
G. Righetti, C. Gelfi, M. Perego, A: V. Stoyanov,A. Bossi,
| |
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|
(Milan,
Italy; Moscow , Russia)
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12.30 - 14.00
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LUNCH
|
PLENARY SESSION 3
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| TRANSBLOT,
DETECTION AND QUANTITATION
|
| Chairpersons:
J.-C. Sanchez, L.Bini
|
14.00 - 14.20
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PARALLEL PROTEIN
CHEMICAL PROCESSING DURING TRANSBLOT
|
| W.
Bienvenut, P.-A. Binz, R.D. Appel, J.-C. Sanchez,K. Rose, J.-L. Veuthey,
| |
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|
D.F.
Hochstrasser (Geneva, Switzerland)
|
14.20 - 14.40
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15N-
AND 13C- LABELING OF CELLS FOR IDENTIFICATION AND
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| QUANTIFICATION
OFPROTEINS ON 2D GELS
| |
| H.Langen,
M. Fountoulakis, S. Evers, B. Wipf, P. Berndt (Basel,Switzerland)
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14.40 - 15.00
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STABLE
ISOTOPE METABOLIC LABELING COUPLED WITH MASS
|
| SPECTROMETRY:
A RIGOROUS METHOD OF QUANTITATIVE
| |
| ANALYSIS
OF PROTEIN EXPRESSION AND INTERMOLECULAR
| |
| INTERACTIONS
| |
| Y.
P. Rochon, E. B. Carmack, J. R. Yates III, R. Aebersold, B. R. Franza, Jr.
| |
| (Seattle,
WA, USA)
|
15.00 - 15.20
|
APPLICATIONS
OF MULTI-PHOTON DETECTION IMAGER TO
|
| DIFFERENTIAL
DISPLAY OF DNA AND PROTEINS
| |
| A.K.
Drukier, Ph. Valadon, A. Wilk, D.Price (Fairfax, VA, USA)
|
PARALLEL SESSION: A
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15.30 - 16.30
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BIOLOGICAL FLUIDS
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|
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Chairpersons:
C.R. Merril, E. Gianazza
|
CORRELATION OF A HUMAN PLASMA PROTEIN MAP WITH A POLYPEPTIDE MAP
|
| BY
TECHNIQUES OF MICRO 2-D ELECTROPHORESIS AND CAPILLARY
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| ELECTROPHORESIS
(15min)
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| T.
Manabe, H. Mizuma, J. Mukai, N. Yamaguchi, N. Naohara (Matsuyama-city,
Japan)
|
IDENTIFICATION OF LOW ABUNDANCE PROTEINS IN CEREBROSPINAL FLUID
AND
|
| USING
PREPARATIVE TWO-DIMENSIONAL LIQUID PHASE ELECTROPHORESIS
|
| MALDI-TOF-MS
(15min)
|
| P.
Davidsson, A. Westman, M. Puchades, C. Nilsson, C. Hesse, K.Blennow
(Mölndal, Sweden)
|
PROTEINS OF RAT SERUM UPON EXPERIMENTAL INFLAMMATION (15 min)
|
| I.
Miller, R. Aebersold, C. Bolego, I. Eberini, P. Haynes, M. Gemeiner, L.Puglisi,
C. R. Sirtori, V.
|
| Zancan,
E.Gianazza (Wien, Austria; Seattle, WA, USA; Milan, Italy)
|
2D ELECTROPHORESIS AND COMPUTER IMAGING: QUANTITATION OF HUMAN
|
| MILK
CASEIN (15 min)
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| M.
Goldfarb (Portland,ME, USA)
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16.30 - 17.00
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COFFEE BREAK
|
17.00 - 17.45
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RAT AND MOUSE
|
|
|
Chairpersons:
J.Klose, P. Mose Larsen
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MAPPING GENES OF GENETICALLY VARIANT MOUSE PROTEINS. COMPARISON
|
| OF
THE MUTAGENIC EFFECT IN DIFFERENT ORGANS. (15 min)
|
| A.
Kaindl, H. Himmelbauer, M. Loewe, H. Lehrach, J.Klose (Berlin,
Germany)
|
TOWARDS UNDERSTANDING DIABETES AT THE MOLECULAR LEVEL (15 min)
|
| P.
Mose Larsen, J. Nerup, A. Karlsen, U. B. Christensen, H. Ullits Andersen,
M. R.Larsen,
|
| P.
Roepstorff, S. J. Fey (Odense; Gentofte; Denmark)
|
A PROTEOMICS ANALYSIS OF LIVERS FROM OBESE (OB/OB) MICE TREATED
WITH
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| THE
PPAR AGONISTS WY14,643 AND BRL49653 (15 min)
|
| U.
Edvardsson, M. Alexandersson, F. Nilsson, H.Brockenhuus von Löwenhielm,
A.-C. Nyström,
|
| B.
Dahllöf (Mölndal, Sweden)
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PARALLEL SESSION: B
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15.30- 16.30
|
PROKARYOTES I
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|
|
Chairpersons:
I.Humphery Smith, M. P. Molloy
|
REPLICATION-INDUCED PROTEIN SYNTHESIS AND ITS IMPORTANCE TO
|
| PROTEOMICS
(15 min)
|
| I.
Humphery-Smith (Eveleigh, Australia)
|
A PROTEOMIC APPROACH TO STUDY THE LEUCINE-RESPONSIVE REGULATORY
|
| PROTEIN
(Lrp) REGULON OF ESCHERICHIA COLI (15 min)
|
| K.
D. Greis, T. H. Tani, R. G. Matthews, R. A.VanBogelen (Ann Arbor, MI,
USA)
|
CHARACTERIZATION OF E. COLI PROTEIN EXPRESSION AT HIGH
CELL DENSITIES
|
| USING
2-D GEL ELECTROPHORESIS (15 min)
|
| B.Franzén,
S. Becker, R. Mikkola , A. Tjernberg, K. Tidblad, S Birnbaum(Sweden)
|
A PROTEOMIC STUDY OF CYANOBACTERIUM SYNECHOCYSTIS SP.
STRAIN PROTEIN
|
| PCC6803:
ANALYSIS OF N-TERMINAL SEQUENCES OF THE CONSTITUENTS OF 224
|
| SPOTS
RESOLVED BY 2D GEL ELECTROPHORESIS, CONSTRUCTION OF A 2D
|
| PROTEIN-GENE
LINKAGE MAP AND ITS USE IN GENE KNOCK-OUT
|
| EXPERIMENTS
(15 min)
|
| T.Sazuka,
M. Asayama, M. Shirai, O. Ohara (Japan)
|
MYCOBACTERIAL PROTEOME ANALYSIS (15 min)
|
| P.R.
Jungblut, U.E. Schaible, H. Mollenkopf, B. Raupach, U.Zimny-Arndt, S.
Lamer, J.Mattow,
|
| S.H.E.
Kaufmann (Berlin,Germany)
|
THE EFFECT OF INTERFERON GAMMA ON CHLAMYDIA
TRACHOMATISPROTEIN
|
| EXPRESSION:
A 2D-PAGE (IPG) ANALYSIS (15 min)
|
| A.C.
Shaw, G. Christiansen, S. Birkelund (Aarhus, Denmark)
|
VIRULENT PHENOTYPES ASSOCIATED WITH THE CAG PATOGENICITY ISLAND
OF
|
| HELICOBACTER
PYLORI (15 min)
|
| L.
Bini,C. Lange, S. Censini, M. Stein, S. Guidotti, R. Rappuoli, V. Pallini,
A.Covacci (Siena, Italy)
|
THE RESPONSE TO FOLATE PATHWAY INHIBITION IN H. INFLUENZAE:
|
| TOWARDS
A BETTER UNDERSTANDING OF ANTIBIOTIC ACTION (15 min)
|
| S.
Evers, K. Di Padova, M. Meyer, M.Fountoulakis, H. Langen, B. Takacs, W. Keck,
C. P. Gray
|
| (Basel,
Switzerland)
|
PRELIMINARY DATA FOR AN INDEX OF HAEMOPHILUS INFLUENZAE
|
| IMMUNOGENIC
PROTEINS (15 min)
|
| B.
Marzocchi, L. Bini, S. Liberatori, B. Magi, R. Raggiaschi, C.Cellesi, V.
Pallini, A. Rossolini
|
| (Siena,
Italy)
|
16.30 - 16.55
|
COFFEE BREAK
|
16.55 - 18.10
|
PROKARYOTES II
|
|
|
Chairpersons:F.C.Neidhardt,
P. Jungblut
|
TUESDAY SEPTEMBER 1st
|
PLENARY SESSION 4
|
| PROTEIN CHEMICAL
ANALYSIS
|
SESSION 4 A
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| PROTEIN
IDENTIFICATION AND PARTIAL CHARACTERISATION
|
| Chairpersons:
F. Lottspeich, S. Patterson
|
9.00 - 9.20
|
MASS SPECTROMETRIC
PROTEIN IDENTIFICATION APPROACHES
|
| FOR
PROTEOMIC ANALYSES
| |
| S
D. Patterson (Thousand Oaks, CA,USA)
|
9.20 - 9.40
|
MALDI-PSD ANALYSIS
ON HIGHLY DILUTED PEPTIDE MIXTURES:
|
|
|
A
HIGHLY SENSITIVE PROTEIN IDENTIFICATION METHOD IN
|
|
|
PROTEOMICS
|
| K.
Gevaert, H. Demol, J.-L. Verschelde, D. Broekaert,S. De Boeck, T.
| |
|
|
Houthaeve,
J. Vandekerckhove (Ghent, Belgium)
|
9.40 - 10.00
|
MASS SPECTROMETRY
OF INTACT PROTEINS FROM 2D GELS
|
|
|
C.
Eckerskorn (Martinsried,Germany)
|
SESSION 4 B
|
| CO-
AND POST-TRANSLATIONAL MODIFICATION ANALYSIS
|
| Chairpersons:
R. Aebersold, N. Packer
|
10.00 - 10.20
|
ANALYSIS OF
POST TRANSLATIONAL MODIFICATIONS IN
|
|
|
PROTEINS
AND PROTEOMES
|
|
|
D.
Goodlett, P. Haynes, G. Corthals, S. Gygi, J. Sherman, R.Aebersold
|
| (Seattle,
WA, USA)
|
10.20- 10.40
|
PROTEOME POST-TRANSLATIONAL
MODIFICATIONS: METHOD
|
| FOR
IDENTIFICATION AND QUANTITATIVE ANALYSIS OF
| |
| PROTEIN
LYSINE METHYLATION USING MALDI-TOF AND AAA
| |
| J.
X. Yan, J.-C. Sanchez, P.-A. Binz, K.L. Williams, D. F.Hochstrasser (Sydney, Australia;
Geneva,
| |
| Switzerland)
|
10.40 - 11.10
|
COFFEE BREAK
|
11.10 - 11.30
|
GLYCOBIOLOGY AND
PROTEOMICS
|
|
|
N.
H.Packer (Sydney, Australia)
|
11.30 - 11.50
|
CHARACTERISATION
OF GLYCOPROTEINS IN THE NUCLEUS AND
|
| THE
EXTRACELLULAR MATRIX OF DICTYOSTELIUM DISCOIDEUM
| |
| USING
TWO DIMENSIONAL GEL ELECTROPHORESIS (2-DE) AND
| |
| CARBOHYDRATE-SPECIFIC
ANTIBODIES
| |
|
|
E.
Jung, A. Bohlker, B. R. Herbert, N. H. Packer, A.A. Gooley, K. L.
|
|
|
Williams
(Sydney, Australia)
|
11.50 - 12.10
|
IDENTIFICATION OF
PHOSPHORYLATION SITES IN PROTEINS BY
|
| AFFINITY
BASED SAMPLE PREPARATION METHODS AND MALDI
| |
|
|
PEPTIDE
MASS
|
| M.
R. Larsen, O. N. Jensen, P. Mose Larsen, S. J.Fey, P. Roepstorff
(Odense,
| |
|
|
Denmark)
|
12.10 - 12.30
|
CHEMICAL CLEAVAGES
& TRUNCATION OF PROTEINS AND
|
|
|
ANALYSIS
OF POST-TRANSLATIONAL MODIFICATION
|
|
|
A.
Tsugita, R. Shen, M. Kamo, T. Kawakami, M. Takayama, K. Miyazaki, T. D.
|
| Matsui
(Tokio, Japan)
|
12.30 - 14.00
|
LUNCH
|
PLENARY SESSION 5
|
| THEORY AND
EXPERIMENT DESIGN
|
| Chairpersons:
L. G. Anderson, G.Gonnet
|
14.00 - 14.20
|
PHARMACEUTICAL PROTEOMICS:
CHARACTERIZATION OF
|
|
|
DISEASE
PROCESSES AND DRUG MECHANISMS THROUGH GENE
|
| EXPRESSION
EFFECTS
| |
| N.L.
Anderson, J. Taylor, J.-P. Hofmann, H. Chen, C.Sims, T. Custer, A.
| |
| McGrath,K.
Lyons, J. Lennon, N.G. Anderson (Rockville, MD, USA)
|
14.20 - 14.40
|
MASS
PROFILINGAT THE CELL LEVEL: MODELLING AND TESTING
|
|
|
G.
H. Gonnet (Zurich, Switzerland)
|
14.40 - 15.00
|
RELATIONSHIP
BETWEEN mRNA AND PROTEIN ABUNDANCE IN
|
|
|
YEAST
|
| S.
P. Gygi, Y. Rochon, B. R. Franza, Jr., G. Corthals, R. Aebersold
| |
| (Seattle,
WA, USA)
|
15.00- 15.20
|
POST-TRANSLATIONAL
MODIFICATIONS AND THEIR IMPACT ON
|
| EFFECTIVE
IDENTIFICATION OF PROTEINS IN DATABASES
| |
|
|
J.
D. Cavalcoli, B. Moldover(Ann Arbor, MI, USA)
|
PARALLEL SESSION: A
|
15.30- 16.30
|
YEAST
|
|
|
Chairpersons:
S.Fey, J. Garrels
|
LARGE SCALE FUNCTIONAL ANALYSIS OF THE YEAST GENOME BY TRANSPOSON
|
| TAGGING
(15 min)
|
| P.
Ross-Macdonald, T. Roemer,A Sheehan, D. Symaniatis, P. Coehlo, S. des Etages,
L. Umansky,
|
| K.
Nelson, S. Agarwal, K. Hager, K. Cheung, P. Miller, D. Kanada, H.Bussey, G. S.
Roeder,
|
| M.
Snyder (USA)
|
INVESTIGATION OF THE YEAST PLEIOTROPHIC DRUG RESISTANCE
|
| SYSTEM
(15 min)
|
| A.
Nawrocki, A. Rogowska, M. R. Larsen,P. Roepstorff, E. Balzi, A. Goffeau,
S. J. Fey, P. Mose
|
| Larsen
(Odense, Denmark; Louvain la Neuve, Belgium)
|
PDR1 AND PDR5 INTERACTIONS ANALYSED BY TWO-DIMENSIONAL GEL
|
| ELECTROPHORESIS
(15 min)
|
| A.
Rogowska, A.Nawrocki, P. Mose Larsen, P. Roepstorff, E. Balzi, A. Goffeau,
S. J.Fey
|
| (Odense,
Denmark; Louvain la Neuve, Belgium)
|
OVER-EXPRESSION IN YEAST OF Stp1 SMALL TYROSINE PHOSPHATASE AND OF
|
| ITS
DOMINANT NEGATIVE C11S. A 2-D ELECTROPHORETIC STUDY (15 min)
|
| A.
Modesti, L. Bini, V. Pallini, L. Carraresi, G.Raugei, G. Ramponi
(Firenze; Siena; Italy)
|
IDENTIFICATION OF YEAST PROTEINS FROM 2-D GELS: WORKING OUT SPOT
|
| CROSS-CONTAMINATION
(15 min)
|
| K.
C.Parker, J. I. Garrels, W. Hines, D. Patterson, S. Martin (Framingham,
Beverly, MA, USA)
|
16.30 - 16.55
|
COFFEE BREAK
|
16.55 - 18.10
|
PLANTS
|
|
|
Chairpersons:
H.Thiellement, V. Stocchi
|
PLANT PROTEOMICS, A NECESSARY COMPLEMENT TO PLANT GENETICS AND
|
| PHYSIOLOGY
(15 min)
|
| H.
Thiellement (Versailles, France)
|
IDENTIFICATION AND CHARACTERIZATION OF WHEAT GRAIN
|
| ALLERGENS
(15 min)
|
| W.
Weiss, B. Scheibe, M.J. Dunn, A. Görg (Munich,Germany; Harefield,
UK)
|
TWO-DIMENSIONNAL ANALYSIS OF HYDROPHOBIC PROTEINS FROM PLANT
|
| PLASMA
MEMBRANE (15 min)
|
| V.
Santoni, D. Rouquié, T. Rabilloud, M. Rossignol,P. Doumas
(Montpellier, Grenoble, France)
|
SEPARATION AND ANALYSIS OF THE PROTEINS OF PHOTOSYNTHETIC
|
| MEMBRANES
OF CYANOBACTERIA AND CHLOROPLASTS (15 min)
|
| P.
R. Chitnis, V. P. Chitnis, J. Sun, D. A. Heck (Ames, IA,USA)
|
SEPARATION AND CHARACTERIZATION OF NEEDLE AND XYLEM MARITIME PINE
|
| PROTEINS
(15 min)
|
| P.
Costa, C. Pionneau, G. Bauw, C.Dubos, N. Bahrman, A. Kremer, J.-M. Frigerio,
C. Plomion
|
|
(Cestas, France; Gent, Belgium)
|
PARALLEL SESSION: B
|
15.30- 16.30
|
EUKARYOTIC CELLS AND TISSUES I
|
|
|
Chairpersons:
M. J. Dunn, R. Zwilling
|
PROTEOMIC APPROACH TO THE STUDY OF HEART DISEASE AND
|
| TRANSPLANTATION
(15 min)
|
| M.
J. Dunn, C. Wheeler, J. Weekes, V. Patel, H.Brittan, M. Heinke, M. Yacoub
(Harefield, UK)
|
VARIABLE LEVELS OF BRAIN PROTEINS RELATED TO DOWN SYNDROME AND
|
| ALZHEIMER'S
DISEASE (15 min)
|
| G.
Lubec, O. Labudova, N. Cairns,M. Fountoulakis (Vienna, Austria;
London, UK; Basel,Switzerland)
|
ERP29, A NOVEL RESIDENT OF THE ENDOPLASMIC RETICULUM: ISOLATION,
|
| CHARACTERISATION
AND TWO-DIMENSIONAL GEL MAPPING (15 min)
|
| M.J.
Hubbard (Dunedin, New Zealand)
|
PROTEOME ANALYSIS OF SIGNAL TRANSDUCTION (15 min)
|
| J.Godovac-Zimmermann
(Jena, Germany)
|
IDENTIFICATION OF PHOSPHOPROTEINS AND THEIR PHOSPHORYLATION SITES
|
| IN
HUMAN PLATELETS BY 2D-PAGE AND MASS SPECTROMETRY (15 min)
|
| D.
Immler, K. Marcus, P. Presek, H. E. Meyer (Bochum, Gießen;
Germany)
|
TRANSLOCATION OF ADP-RIBOSYLATION FACTOR SMALL GTPASES IS
|
| REGULATED
BY GTP/GDP-CYCLE AND V-TYPE ATPASEDEPENDENT
|
| ENDOSOMAL ACIDIFICATION
(15 min)
|
| V.
Marshansky, B. Maranda (Montréal, Canada)
|
ASPARTIC PROTEINASES IN C. ELEGANS: ISOLATION, IDENTIFICATION
AND GEL
|
| CHARACTERIZATION
BY A COMBINED USE OF AFFINITY CHROMATOGRAPHY, 2D
|
| ELECTROPHORESIS.
MICROSEQUENCING AND DATABANK ANALYSIS (15 min)
|
(Heidelberg, Germany; Siena, Italy)
|
2-DTYROSINE-PHOSPHORYLATION PROFILES IN GUINEA PIG BRAIN
|
| SYNAPTOSOMES
(15 min)
|
| S.
Liberatori, G.Buonocore, L. Bini, S. Zanelli, J. A. Kubin, O. P. Mishra,
M.Delivoria-
|
| Papadopoulos,
V. Pallini, R. Bracci (Siena, Italy; Philadelphia,PA, USA)
|
16.30 - 16.55
|
COFFEE BREAK
|
.55 - 17.55
|
EUKARYOTIC CELLS
AND TISSUES II
|
|
|
Chairpersons:
J.-D. Tissot, R. J. Simpson
|
19.30
|
SOCIAL DINNER
|
WEDNESDAY SEPTEMBER 2nd
|
PLENARY SESSION 6
|
| PROTEOME
DATA MANAGEMENT, STORAGE AND DISPLAY
|
| Chairpersons:
A. Bairoch, S. Prime
|
9.00 - 9.20
|
BIOINFORMATICS FOR
PROTEOME ANALYSIS IN SWITZERLAND
|
|
|
A.
Bairoch, R.D. Appel, R.E. Offord, D.F.Hochstrasser(Geneva,
Switzerland)
|
9.20 - 9.40
|
DATA
REDUCTION FOR COMPARATIVE PROTEIN EXPRESSION
|
| ANALYSIS
| |
|
|
S.
Prime , R. Wilson (Oxon, UK)
|
9.40 - 10.00
|
SWISS-2DPAGE,MY-2DPAGE,
YOUR-2DPAGE...: HOW TO PUBLISH
|
|
|
2-DE
DATA ON THE WEB
|
| C.
Hoogland, J.-C. Sanchez, L. Tonella, D.F. Hochstrasser, R.D. Appel
| |
| (Geneva,
Switzerland)
|
PLENARY SESSION 7
|
| ARTIFICIAL
INTELLIGENCE AND NEURAL NETWORKS
|
| Chairpersons:
R. D. Appel, P. J.Drew
|
10.00 - 10.20
|
NEURAL NETWORKS:
THEORY AND APPLICATION TO COMPLEX
|
| PROBLEM
SOLVING
| |
|
|
P.
J. Drew (U.K.)
|
10.20 - 10.40
|
NEW
ALGORITHMIC APPROACHES FOR PROTEIN SPOT DETECTION
|
|
|
AND
POINT PATTERN MATCHING OF 2-DE GEL DATABASES
|
|
|
K.-P.
Pleissner, K. Kriegel, F. Hoffmann, C. Wenk, S. Wegner, H. Oswald,H.
|
| Alt,
E. Fleck (Berlin, Germany)
|
10.40 - 11.00
|
2-DE CLASSIFICATION
USING HEURISTIC CLUSTERING AND
|
|
|
NEURAL
NETWORKS
|
| P.M.
Palagi, D. Walther, G. Bologna, J.R. Vargas, J-.C. Sanchez, O. Cremona,
| |
| D.F.
Hochstrasser, R.D. Appel (Geneva, Switzerland; Cochabamba, Bolivia; Torino,
Italy)
|
11.00 - 11.30
|
COFFEE BREAK
|
PLENARY SESSION 8
|
| INTERVENTIONAL
CLINICAL AND BIOMEDICAL PROTEOMICS
|
| Chairpersons:
J. E. Celis, M. G. Harrington
|
11.30 - 11.50
|
A
PROTEOMIC APPROACH TO THE STUDY OF CANCER
|
|
|
J.
E. Celis, M. Østergaard, H. H. Rasmussen, I.Gromova, H. Wolf, P.
Gromov
|
|
|
Williams
(Aarhus, Denmark)
|
11.50 - 12.10
|
MINING
THE HUMAN PROTEOME: EXPERIENCE WITH THE HUMAN
|
| LYMPHOID
PROTEIN DATABASE
| |
|
|
S.M.
Hanash, D. Teichroew (Ann Arbor, MI,USA)
|
12.10 - 12.30
|
EXPLORING
BRAIN PATHOPHYSIOLOGY
|
| M.
G.Harrington (Pasadena, CA , USA)
|
12.30 - 12.50
|
PROTEIN
LEVEL DIFFERENCES IN POST MORTEM FRONTAL LOBES
|
| OF
THE MENTALLY ILL
| |
|
|
N.
Johnston, N. Anderson, L. Anderson, A. Shore, R Yolken, E. F. Torrey,
|
| and
the Stanley Neurovirology Consortium (Baltimore, MD;Rockville, MD; USA)
|
12.50 - 14.30
|
LUNCH
|
14.30 - 16.30
|
VISIT
AND DISCUSSION TO POSTERS - COFFEE BREAK
|
PARALLEL SESSION: A
|
16.30 - 18.15
|
ONCOLOGY
|
|
|
Chairpersons:
S.Hanash, C. Sarto
|
AN INTRONIC SEQUENCE ELEMENT MEDIATES BOTH ACTIVATION AND SPLICING
|
| REPRESSION
OF RAT FIBROBLAST GROWTH FACTOR RECEPTOR 2 PRE-mRNA
|
| IN
A PROSTATE CANCER MODEL SYSTEM (15min)
|
| M;
A. Garcia-Blanco, R. P. Carstens, E. Wagner (Durham, NC,USA)
|
TWO-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF CRYOPROTEINS: A REPORT
|
| OF
334 CASES (15 min)
|
| J.-D.
Tissot, F. Invernizzi, J. Schifferli, F. Spertini, P.
Schneider(Lausanne, Switzerland; Milan, Italy;
|
| Basel,
Switzerland)
|
MODIFIED EXPRESSION OF PLASMA GLUTATHIONE PEROXIDASE AND
|
| MANGANESE
SUPEROXIDE DISMUTASE IN HUMAN RENAL CELL
|
| CARCINOMA
(15 min)
|
| C.
Sarto, S. Frutiger, F. Cappellano, J.-C. Sanchez, G. Doro, F. Catanzaro,
G. J.Hughes, D. F.
|
| Hochstrasser,
P. Mocarelli (Desio-Milan, Italy; Geneva, Switzerland)
|
CHARACTERIZATION OF ASTROCYTOMA AND OLIGODENDROGLIOMA-SPECIFIC
|
| PROTEINS
BY TWO-DIMENSIONAL ELECTROPHORESIS (15 min)
|
| Th.M.
Luider, P.A.E. Sillevis Smitt, M.J. van den Bent, Ch.J. Vecht,J.M. Kros
(Rotterdam, Nederland)
|
IDENTIFICATION OF PROTEIN ALTERATIONS IN HUMAN PROSTATE EPITHELIAL
|
| TUMOR
CELLS UNDERGOING IONIZING RADIATION-INDUCED
|
| APOPTOSIS
(15 min)
|
| SC
Prasad, PJ Thraves, V Soldatenkov, P Ramsamooj, Z Xiaojun, MK Kuettel,
A.Dritschilo
|
| (Washington
DC, USA)
|
BIOCHEMICAL CHARACTERIZATION OF AN ANTIGEN RECOGNIZED BY THE IGM
|
| PRODUCED
BY MALIGNANT CELLS OF A BURKITT LYMPHOMA (15 min)
|
| L.
Musante, M. Ulivi, G. Cutrona, S. Roncella, G.Candiano, M. Ferrarini
(Genova, La Spezia; Italy)
|
PROTEOMICS OF HUMAN DISEASE (15 min)
|
| J.
Harry, K. Giese, B. Herbert, M. Tyler, A. Gooley,M. Traini, D. Mitchell, M.
Wilkins, J. Yan, N.
|
| Packer,
K. Ou, S. Gates, M. Molloy, M. Lawson, S. Kaur, B.Walsh, K. Williams
|
| (Sydney,
Australia; Emeryville, CA, U.S.A)
|
PARALLEL SESSION: B
|
16.30 - 17.30
|
SAMPLE
PREPARATION AND MASS SPECTROMETRY RELATED
|
| TECHNOLOGIES
| |
| Chairpersons:
P.-A. Binz, H. Langen
|
STRATEGIES FOR IMPROVED SEPARATION OF HYDROPHOBIC PROTEINS ON 2-D
|
| GELS
FOR PROTEOME PROJECTS: INCREASING PROTEIN SOLUBILITY AND
|
| SEQUENTIAL
EXTRACTION (15 min)
|
| M.
P. Molloy, B. R. Herbert, T. Rabilloud, K. L. Williams, A. A. Gooley
|
| (Sydney,
Australia; Grenoble, France)
|
CAPILLARY COLUMN CHROMATOGRAPHY IMPROVES SAMPLE PREPARATION FOR
|
| MASS
SPECTROMETRIC ANALYSIS: COMPLETE CHARACTERIZATION OF HUMAN
|
| ENOLASE
FROM TWO-DIMENSIONAL GELSFOLLOWING IN-SITU PROTEOLYTIC
|
| DIGESTION
(15 min)
|
| R.
J. Simpson, R. K. Rasmussen, D. S. Dorow, G. E.Reid (Parkville,
Melbourne; Australia)
|
MODIFIED IN-GEL PROTEIN DIGESTION PROCEDURE ALLOWS HIGH THROUGHPUT
|
| MS
ANALYSIS FOLLOWING 2-D GEL GRIDDING (15 min)
|
| H.
Langen, M. Fountoulakis, P. Berndt (Basel,Switzerland)
|
MICROSEQUENCE ANALYSIS OF PROTEINS FROM A SINGLE 2D-GEL WITH AN
ION-
|
| TRAP
MASS SPECTROMETER
|
| G.
Bauw, E. Posada, C. Gilot,A. Lenaerts, M. Davey, M. Van Montagu (Gent,
Belgium)
|
17.30 - 18.00
|
PHARMACOLOGY AND
TOXICOLOGY
|
|
|
Chairpersons:
L. G. Anderson, F. A. Witzmann
|
APPLIED PROTEOMICS - INVESTIGATING THE NEPHROTOXICITY OF
|
| LEAD
(15 min)
|
| F.
A. Witzmann, C. D.Fultz, R. A. Grant, S. E. Kornguth, L. S. Wright, F. L.
Siegel
|
| (Columbus,
IN; Madison WI; USA)
|
COMPARATIVE ANALYSIS OF BACTERIAL RESPONSES AS A TOOL FOR THE
|
| PREDICTION
OF ANTIBIOTIC MODE OF ACTION (15 min)
|
| S.Evers,
K. Di Padova, M. Fountoulakis, H. Langen, B. Takacs, W. Keck, C. P.Gray
(Basel, Switzerland)
|
THURSDAY SEPTEMBER 3rd
|
PLENARY SESSION 9
|
| NANOTECHNOLOGY
- MULTIDIMENSIONAL SEPARATION ON A CHIP AND
MICROCHIP CHEMISTRY
|
| Chairpersons:H.
H. Girault, J. Roeraade
|
9.00 - 9.20
|
MICRO-CHANNELS NETWORKS
FOR ELECTROPHORETIC
|
|
|
SEPARATIONS
|
| F.
Bianchi, R. Ferrigno,J. Rossier, A. Schwarz, H. H. Girault
| |
| (Lausanne,
Swittzerland)
|
9.20 - 9.40
|
TO
BEANNOUNCED
|
|
|
J.Roeraade
|
9.40 - 10.00
|
MICROSAMPLE HANDLING
ON MICROFABRICATED DEVICES FOR
|
| AUTOMATED,
HIGH SENSITIVITY IDENTIFICATION OF
| |
| PROTEINS
BY ELECTROSPRAY IONIZATION TANDEM MASS
| |
| SPECTROMETRY
| |
| D.
Figeys, R.Aebersold (Halifax, Canada; Seattle, WA, USA)
|
PLENARY SESSION 10
|
| CYBERPROTEOMIC
TOOLS
|
| Chairpersons:
M. R. Wilkins, J.Yates
|
10.00- 10.20
|
MINING
THE PROTEOME WITH COMPUTERS AND TANDEM MASS
|
| SPECTROMETRY
| |
|
|
J.
Yates (Seattle, WA, USA)
|
10.20- 10.40
|
PROTEIN MODIFICATIONS,
PROCESSING AND IDENTIFICATION
|
| IN
PROTEOME PROJECTS
| |
| M.
R.Wilkins, E. Gasteiger, D. K. Sexton, B. R. Herbert, M. P. Molloy, K. Ou,
| |
| P.-A.
Binz, A. Bairoch, R. Appel, A. A. Gooley, D. F.Hochstrasser, K. L.
| |
|
|
Williams
(Sydney, Australia; Geneva , Switzerland)
|
10.40 - 11.00
|
SOFTWARE
TOOLS FOR AUTOMATED PROTEIN DATABASE
|
| SEARCHES
WITH PEPTIDE MASS FINGERPRINTS
| |
|
|
U.
Hobohm, P. Berndt, H. Langen (Basel, Switzerland)
|
11.00 - 11.30
|
COFFEE BREAK
|
PLENARY SESSION 11
|
| AUTOMATION
AND THE FUTURE
|
| Chairpersons:
P. James, A. A.Gooley
|
11.30 - 11.50
|
VISUALIZATION OF
THE E. COLI PROTEOME WITH MASS
|
| SPECTROMETRY
| |
| R.
R. Ogorzalek Loo, C. Mitchell, P. C.Andrews (Ann Arbor, MI, USA)
|
11.50 - 12.10
|
CHEMICAL PRINTING,
A NEW PARADIGM IN LARGE-SCALE
|
|
|
PROTEOMICS:
APPLICATIONS TO PEPTIDE MASS
|
| FINGERPRINTING
| |
| A.
A. Gooley, M. Traini, F. Hopwood (Sydney, Australia)
|
12.10 - 12.30
|
PROTEOME ANALYSIS
AUTOMATION
|
|
|
P.
Dainese,S. Hoving, M. Munchbach, W. Staudenmann, P. James
|
| (Zurich,Switzerland)
|
12.30- 12.50
|
THE
"MOLECULAR SCANNER": AN HIGHLY AUTOMATED METHOD
|
|
|
FOR
PROTEIN IDENTIFICATION AND 2-D PAGE ANNOTATION
|
| P.-A.
Binz, W. Bienvenut, R. Fabbretti, E. Gasteiger, A. Bairoch, R. Appel,
| |
| J.-
C. Sanchez, D. F. Hochstrasser(Geneva, Switzerland)
|
12.50- 14.30
|
LUNCH
|
CLOSING LECTURE
|
14.30 - 15.15
|
GENOMICS,COMBINATORIAL
CHEMISTRY, GENE MANIPULATION
|
|
|
IN
VIVO, AND PROTEOMICS: KEY TECHNOLOGIES FORSTUDYING
|
| COMPLEX
BIOLOGICAL PHENOMENA
| |
|
|
K.
L.Williams (Sydney, Australia)
|
15.15 - 15.30
|
CONCLUDING REMARKS.
|
|
|
D.F.Hochstrasser,
V. Pallini
|
Posters plenary session 1: SAMPLE PREPARATION AND SOLUBILISATION
|
PROTOCLEAR TM ALLOWS DETECTION OF RARE SERUMPROTEINS
|
| B.
A. Lollo, S. Harvey, R. Sayen, R. Wagenknecht, J. Liao, J. Whaley and F.G.
Sajjadi
|
| (San
Diego, CA, USA)
|
Posters plenary session 2: pH, pI, pK AND RELEVANT GRADIENTS
|
2D ELECTROPHORESIS OF PROTEINS IN AN IMMOBILIZED pH 3-12 GRADIENT
|
| G.
Boguth, A. Harder, C. Obermaier, R. Wildgruber, A. Görg (Munich,
Germany)
|
TOWARDS AUTOMATIZATION OF 2D ELECTROPHORESIS: THE IPGPHOR
|
| C.
Obermaier, G. Boguth, A. Harder, R. Wildgruber, A. Görg (Munich,
Germany)
|
FASTER,SIMPLER 2-D ELECTROPHORESIS USING A NEW INSTRUMENT FOR
FIRST
|
| DIMENSION
IEF
|
| T.
Berkelman, N. Laird, W.Stochaj, E. Hungerman, M.-I. Benito, W. Gurske, K.
Lee-Tung, C. Ko, J.
|
| Fichmann,
R. Islam (San Francisco, CA, USA)
|
2-D PAGE PROTOCOLS: MODIFIED IPG GEL STRIP EQUILIBRATION PROCEDURE
|
| J.
X. Yan, J.-C. Sanchez, V. Rouge, K.L. Williams, D. F. Hochstrasser (Sydney,Australia;
Geneva, Switzerland)
|
Posters plenary session 3: TRANSBLOT DETECTION AND QUANTITATION
|
PRACTICAL ASPECTS OF FLUORESCENT STAINING FOR PROTEOMIC
|
| APPLICATIONS
|
| J.
P. Malone, G. S. Gerstenecker, R. M. Leimgruber (USA)
|
QUANTITATION IN PROTEOME STUDIES: APPLICATION OF AMINO ACID
|
| ANALYSIS
TO SACCHAROMYCES CEREVISIAE PROTEOME EXPRESSION
|
| J.
X. Yan, J.-C. Sanchez, L. Tonella, K.L. Williams, D. F. Hochstrasser(Sydney, Australia;
Geneva, Switzerland)
|
Posters plenary session 4A: PROTEIN IDENTIFICATION AND PARTIAL CHARACTERISATION
|
OPTIMIZING PROTEIN IDENTIFICATION AT SUB-PICOMOLE LEVELS FROM TWO-
|
| DIMENSIONAL
GELS BLOTTED TO PVDF MEMBRANES FOR PROTEOME STUDIES
|
| D.
W. Speicher, D. F. Reim, K. D. Speicher, P. Hembach(Philadelphia, PA,
USA)
|
Posters plenary session 4B: CO- AND POST-TRANSLATIONAL MODIFICATION ANALYSIS
|
ANALYSIS OF POST-TRANSLATIONAL MODIFICATION SITES USING CHEMICAL
|
| CLEAVAGES
AND MASS SPECTROMETRY
|
| M.
Kamo, T.Kawamaki, A. Tsugita (Tokyo, Japan)
|
Posters plenary session 10: CYBER PROTEOMIC TOOLS
|
SOFTWARE TOOLS FOR AUTOMATED ANALYSIS OF PEPTIDE MAP MASS SPECTRA
|
| P.
Berndt, H. Langen (Basel, Switzerland)
|
TOWARDS FULLY AUTOMATED IDENTIFICATION OF PROTEINS ON 2-D PAGE GELS
|
| R.
Fabbretti, P.-A. Binz, W. Bienvenut, E. Gasteiger, A.Bairoch, M. R.Wilkins,
J.-C. Sanchez, D.
|
| Walther,
D. F. Hochstrasser, R. D. Appel (Geneva, Switzerland;Sydney, Australia)
|
Posters plenary session 11: AUTOMATION AND THE FUTURE
|
TOWARDS AN INTEGRATED HIGH-THROUGHPUT SYSTEM FOR PROTEOME
|
| ANALYSIS
|
| M.
Traini, F. Hopwood, M. P. Molloy, K. Ou,Bradley J. Walsh, M. R. Wilkins, K. L.
Williams, A.
|
| A.Gooley
(Sydney, Australia)
|
Posters parallel session : BIOLOGICAL FLUIDS
|
ANALYSIS OF TEAR PROTEIN PATTERNS BY A NEURAL NETWORK AS A
|
| DIAGNOSTICAL
TOOL FOR THE DETECTION OF DRY EYES
|
| F.H.Grus,
A.J. Augustin (Bonn, Germany)
|
IDENTIFICATION OF PROTEINS IN A PLEURAL EXUDATE USING PREPARATIVE
|
| 2-DIMENSIONAL
LIQUID PHASE ELECTROPHORESIS AND MALDI TOF MS
|
| C.
L. Nilsson, P. Davidsson, M. Puchades, A. Westman, R.Ekman (Mölndal,
Sweden)
|
2-DE PROTEIN PATTERNS OF HUMAN NASAL LAVAGE FLUIDS. AFTER EXPOSURE
|
| CHARACTERIZATION
OF CHANGES IN PATIENTS WITH ALLERGIC RHINITIS AND
|
| TO
IRRITATING CHEMICALS
|
| J.
Svartz, B. Ståhlbom, C. Tagesson, M. Lindahl (Linköping,
Sweden)
|
COMPUTERIZED 2-D ELECTROPHORESIS OF BAL PROTEINS: A NEW APPROACH
|
| FOR
THE STUDY OF LUNG DISEASES
|
| B.
Magi, L. Bini, S. Liberatori, B. Marzocchi, P. Rottoli, M. G. Perari,
M.Vagliasindi , V. Pallini
|
| (Siena,
Italy)
|
BULL SEMINAL PLASMA PROTEIN MAP: UPDATING AND CHARACTERIZATION OF
|
| A
NOVEL CUB DOMAIN FAMILY-ASSOCIATED PROTEIN BY IN-GEL DIGESTION
|
| AND
MICROSEQUENCING
|
| M.
Mortarino, F. Ceciliani, G. Tedeschi, A. Negri, L. Gottardi, G. Maffeo,S.
Ronchi (Milan, Italy)
|
TWO-DIMENSIONAL PROTEIN MAPS OF BOVINE OVARIAN FLUIDS
|
| M.
Mortarino, D. Vigo, F. Ceciliani, G. Tedeschi, P.A. Biondi, S. Ronchi,G. Maffeo
(Milan, Italy)
|
Posters parallel session : RAT AND MOUSE
|
MAPPING GENES OF POLYMORPHIC AND NON-POLYMORPHIC PROTEINS FROM
|
| MOUSE
BRAIN 2DE GELS
|
| C.
Nock, C. Gauss, L. Schalkwyk, H. Lehrach, H.Himmelbauer, J. Klose (Berlin,
Germany)
|
EPIGENETIC EFFECTS OBSERVED IN 2-D PROTEIN PATTERNS OF MOUSE
EMBRYOS
|
| (DAY-10)
DERIVED FROM ENU-TREATED PATERNAL SPERMATOGONIA
|
| J.Nowak,
C. Gericke, J. Prehm, J. Klose (Berlin, Germany)
|
A TWO-DIMENSIONAL ELECTROPHORESIS DATABASE OF RAT HEART PROTEINS
|
| Xinping
Li, K.-P. Pleissner, V. Regitz-Zagrosek, P. Salnikof, P. Jungblut(Berlin,
Germany)
|
THE MOUSE SWISS-2DPAGE DATABASE: A BRIDGE BETWEEN GENOMIC AND
|
| PHENOMIC
ANALYSIS OF NON-INSULIN-DEPENDENT DIABETES-MELLITUS
|
| J.-C.
Sanchez, V. Rouge, S. Frutiger, G.Hughes, J. X. Yan, C. Hoogland, R.D. Appel,
P.-A. Binz,
|
| D.F.
Hochstrasser, M. Cowthorne(Geneva, Switzerland; Buckingham, UK)
|
Posters parallel session : PROKARYOTES
|
HTTP://PC13MI.BIOLOGIE.UNI-GREIFSWALD.DE/SUB2D/SUB2D.HTM -
|
| THE
"SUB2D" PROTEIN INDEX OF BACILLUS SUBTILIS
|
| J.
Bernhardt, H. Werner, H. Antelmann, U.Völker, A. Völker, S.
Ohlmeier, U. Zuber, R. Schmid,
|
| H.
Mach, M. Hecker (Greifswald; Marburg; Osnabrück; Germany)
|
A DYNAMIC 2-D PAGE DATABASE: THE MYCOBACTERIAL PROTEOME ON THE
|
| INTERNET
|
| H.-J.Mollenkopf,
B. Raupach, U. Schaible, P.R. Jungblut, S. H. E. Kaufmann(Berlin,
Germany)
|
'98 Escherichia coli SWISS-2DPAGE DATABASE UPDATE
|
| L.
Tonella, B. J. Walsh, J.-C. Sanchez, K. Ou, M. R. Wilkins, M. Tyler,
S.Frutiger, P. -A. Binz, A.
|
| A.Gooley,
I. Pescaru, R. D. Appel, J. X. Yan, A. Bairoch, C.Hoogland, G. J.Hughes, K. L.
|
| Williams,
D. F. Hochstrasser (Geneva , Switzerland;Sydney, Australia)
|
THE USE OF 2D ELECTROPHORESIS METHOD AS A TOOL TO UNDERSTAND
|
| PHYSIOLOGICAL
EVENTS IN ESCHERICHIA COLI
|
| F.
Biville, M. Perrotte, E. Turlin,A. Danchin (Paris, France)
|
PROTEOME ANALYSIS OF FIS OVEREXPRESSION IN E. COLI
|
| R.
Richins, W. Chen, L. H. Choe, and K. H. Lee (Riverside, CA;Ithaca, NY;
USA)
|
IDENTIFICATION OF A PROTEIN FAMILY SPECIFIC FOR RABBIT
|
| ENTEROPATHOGENIC
ESCHERICHIA COLI
|
| D.
Vandekerchove, A. Callebaut, T.S. Agin (Brussels; Tervuren; Belgium.
Washington, USA)
|
ANALYSIS OF THE REGULON OF SALMONELLA PATHOGENICITY ISLAND II, A
|
| NEW
VIRULENCE FACTOR OF SALMONELLA TYPHIMURIUM
|
| J.Deiwick
(Munich, Germany)
|
USE OF HIGH RESOLUTION TWO-DIMENSIONAL (2D) GEL ELECTROPHORESIS AS
|
| ANALYTICAL
TOOL FOR EVALUATION OF IN VITRO PROTEIN BIOSYNTHESIS
|
| P.
T. Schindler, F. Macherhammer,M. Siemann-Herzberg, M.S. Reuss (Stuttgart,
Germany)
|
THE USE OF PROTEOMICS TECHNIQUES TO IDENTIFY S. AUREUS
PROTEINS
|
| L.D.
Adams, G.S. Cavey, J.W. Leone,C.A. Baker (Kalamazoo, MI,USA)
|
HIGH RESOLUTION ANALYSIS OF CYTOPLASMIC AND ENVELOPE ASSOCIATED
|
| PROTEINS
FROM STAPHYLOCOCCUS AUREUS USING 2D GEL ELECTROPHORESIS R.
|
| AND
MASS SPECTROMETRY
|
| F.
Zappacosta, R. S. Annan, J. Lonsdale, and S. A. Carr (King ofPrussia, PA,
USA)
|
ENRICHMENT AND PURIFICATION OF PROTEINS OF
HAEMOPHILUS INFLUENZAE
|
| BY
POLYBUFFER EXCHANGER CHROMATOGRAPHY
|
| M.Fountoulakis,
H. Langen, C. Gray, B. Tak[daggerdbl]cs (Basel,Switzerland)
|
INDUCTION OF HEAT SHOCK PROTEINS IN RESPONSE TO PRIMARY ALCOHOLS
IN
|
| ACINETOBACTER
CALCOACETICUS
|
| D.
Benndorf, N.Loffhagen, W.Babel (Leipzig, Germany)
|
DIFFERENTIAL EXPRESSION OF BACTEROIDS PROTEINS OF
RHIZOBIUMETLI
|
| DURING
SIMBIOSIS WITH PHASEOLUS VULGARIS
|
| S.
Encarnación, M. Hernández, T. Hernández, S.Contreras, Y.
Mora, G. Dávila, J. Mora
|
| (Cuernavaca
Mor., México)
|
MAPS OF PROTEINS INDUCED FOR RHIZOBIUM ETLI IN FREE LIFE IN
THE
|
| AEROBIC
AND FERMENTATIVE METABOLISM
|
| S.
Encarnación, G. Mendoza, M. Hernández, M. del C.Vargas, A.
Dávalos, H. Taboada, M. Dunn, G.
|
| Guerrero,
J. Mora (Cuernavaca Mor.,México)
|
Posters parallel session : YEAST
|
SAMPLE PREPARATION OF SACCHAROMYCES CEREVISIAE PROTEINS FOR
TWO
|
| DIMENSIONAL
ELECTROPHORESIS
|
| R.Wildgruber,
A. Harder, A. Nawrocki, P. Mose Larsen, S.J. Fey, A.Görg
|
| (Munich,
Germany, Odense, Denmark)
|
PROTEOME ANALYSIS, A NOVEL APPROACH FOR TAXONOMIC STUDIES
|
| R.
Joubert, C. Lehmann, C. Monribot, P. Brignon, F. Gendre, H.
Boucherie(Strasbourg; Bordeaux; France)
|
TWO-DIMENSIONAL GEL ELECTROPHORESIS AS ANALYTICAL TOOL FOR THE
|
| STUDY
OF IN VIVO METABOLIC DYNAMICS IN SACCHAROMYCES CEREVISIAE
|
| F.
Macherhammer, P.T. Schindler, M. Siemann, S. Vaseghi, M.Rizzi, M. Reuss
(Stuttgart, Germany)
|
ANALYSIS AND IDENTIFICATION OF PROTEINS SECRETED BY REGENERATING
|
| PROTOPLASTS
OF SACCHAROMYCES CEREVISIAE
|
| M.
Pardo, L. Monteoliva, J. Pla, M.Sanchez, C. Gil, C. Nombela (Madrid,
Spain)
|
ANALYSIS OF OXIDATIVE STRESS RESPONSE IN YEAST BY 2D GEL
|
| ELECTROPHORESIS
|
| C.
Godon, G. Lagniel, J. Lee, J. M.Buhler, S. Kieffer, M. Perrot, H. Boucherie, M.
B. Toledano, J.
|
| Labarre
(Gif-sur-Yvette; Bordeaux; Grenoble; France. Rutgers, USA)
|
Posters parallel session : PLANTS
|
2-DIMENSIONAL ELECTROPHORESIS AS A TOOL FOR PROTEIN DIFFERENTIAL
|
| DISPLAY
IN BIOLOGICAL RESEARCH
|
| J.
Weinman, S. Natera, N. Imin, J. Stubbs, M. Vidovic,N. Guerreiro, M.Djordjevic,
B. Rolfe
|
| (Canaberra,
Australia)
|
USE OF AN ORGANELLE PROTEOME STRATEGY FOR TAGGING OF PROTEINS
|
| PRESENT
AT THE PLANT PLASMA MEMBRANE
|
| V.
Santoni , D. Rouquié, P. Doumas, M. Mansion, M.Boutry, H. Degand, P.
Dupree, L. Packman, G.
|
| Bauw,
E. Posada, P. Rouzé4, P. Dehais, I. Sahnoun, M.
|
| Rossignol(Montpellier,
France; Louvain-la-Neuve; Gent; Belgium; Cambridge,UK)
|
TWO-DIMENSIONAL PROTEIN ELECTROPHORESIS STUDY OF ZYGOTIC AND
|
| SOMATIC
EMBRYOGENESIS IN CYPRESS (CUPRESSUS SEMPERVIRENS L.)
|
| A.
Sallandrouze, M. Faurobert, M. El Maataoui, H. Espagnac(Avignon, France)
|
QUANTITATIVE ANALYSIS OF MARITIME PINE PROTEIN EXPRESSION IN TWO
|
| CONTRASTED
ENVIRONMENTS
|
| P.
Costa, C. Dubos, A. Kremer, C.Plomion (Cestas, France)
|
PROTEOME ANALYSIS OF TWO PLANTS, RICE AND ARABIDOPSIS
|
| T.
Kawakami, M. Kamo, L.-J. Yang, A. Tsugita (Tokyo, Japan)
|
TWO-DIMENSIONAL GEL ELECTROPHORESIS OF ARABIDOPSIS
THALIANAFIRST
|
| PROTEINS;
CARRIER AMPHOLINES VERSUS IMMOBILIZED PH-GRADIENT GELS AS
|
| DIMENSION
|
| G.
Bauw, E. Posada, C. Gilot, A. Lenaerts, M. Van Montagu (Gent,Belgium)
|
2D-PAGE ANALYSIS OF TUBER BORCHII VITTAD MYCELIUM
|
| F.
Bernardini, L. Vallorani, C. Sacconi, B. Pieretti, G. Piccoli, R.Pierleoni, V.
Stocchi (Urbino, Italy)
|
Posters parallel session : EUKARYOTIC CELLS AND TISSUES
|
PEPTIDE-INDUCED ANTIBODIES AS A TOOL TO LINK GENOMES AND PROTEOMES
|
| T.
Fröhlich, G. J. Arnold (Munich, Germany)
|
TRANSLATIONALLY CONTROLLED TUMOR PROTEIN IS PRODUCED IN AND
|
| SECRETED
FROM BASOPHIL LEUCOCYTES. UPREGULATION BY IL-3
|
| H.
V. Nielsen, A. Johnsen, J.-C. Sanchez, D. F.Hochstrasser, P. Oluf Schiøtz
|
| (Aarhus;
Copenhagen, Denmark. Geneva,Switzerland)
|
ACTIVE DISSOCIATION OF HOECHST FROM DNA IN A LIVING CELL. WHO COULD
|
| DO
IT?
|
| S.
Naryzhny, V. Levina, M. Filatov (Gatchina,Russia)
|
TWO-DIMENSIONAL ANALYSIS OF SMALL GTPASES ASSOCIATED WITH
|
| PHAGOSOMES
|
| M.Meyer,
A. Tiedtke (Münster, Germany)
|
A PROTEOMICS APPROACH TO IDENTIFY PACLITAXEL-INDUCED CHANGES IN
|
| BCL-2
OVEREXPRESSING CELLS
|
| D.
Müller, P. Schindler, H. Voshol, J. van Oostrum(Basel, Switzerland)
|
VALIDATION OF A TWO-DIMENSIONAL GEL ELECTROPHORESIS APPROACH FOR
|
| STUDYING
THE VARIATIONS OF PROTEIN EXPRESSION RELATED TO THE
|
| INHIBITION
OF NFkB IN LYMPHOBLASTOIDCELL LINES OVEREXPRESSING IkBa
|
| R.
Joubert-Caron, J. Feuillard, S. Kohanna, J-P. Le Caër 2, F. Poirier,D.
Bladier, M. Raphaël, M.
|
| Caron
(Bobigny; Paris; France)
|
LOOKING FOR PROTEIN KINASE C SUBSTRATES IN ISOFORM OVEREXPRESSING
|
| CELL
LINES BY IN VIVO LABELING AND PROTEOME ANALYSIS
|
| S.Dammeier,
J. Lovric, H. Mischak (Munich; Berlin; Germany)
|
PROBING CGMP-DEPENDENT PROTEIN KINASE SIGNAL TRANSDUCTION
|
| PATHWAYS
USING THE PROTEOMIC APPROACH
|
| F.
Autelitano, J.P. Ledig, C. Déon, A. Buzy, C. Tardif,D. Loyaux, M. Teufel
|
| (Strasbourg;
Rueil Malmaison; France)
|
MAPPING AND IDENTIFICATION OF IFN-GAMMA-REGULATED HELA CELL
|
| PROTEINS
SEPARATED BY IMMOBILIZED PH-GRADIENT TWO-DIMENSIONAL GEL
|
| ELECTROPHORESIS
|
| A.
C. Shaw, M. Larsen, J. Justesen,G. Christiansen, S. Birkelund (Odense;
Aarhus; Denmark)
|
PROTEOME PROFILING OF HUMAN T LYMPHOCYTES STIMULATED WITH
|
| INTERFERON-a
AND INTERLEUKIN-18
|
| T.
Nyman, S. Matikainen, T. Sareneva, I. Julkunen, N. Kalkkinen (Helsinki,
Finland)
|
THE N-TERMINUS OF GP130 IS CRITICAL FOR THE FORMATION OF THE HIGH
|
| AFFINITY
INTERLEUKIN-6 RECEPTOR COMPLEX
|
| R.
L.Moritz, L. D. Ward, T. Guo-Fen, L. J. Fabri, H. Ji, R. J. Simpson
(Parkville; Burnley; Australia)
|
A PROTEOME APROACH TO CYTOKINE MEDIATED ß-CELL DESTRUCTION
AND
|
| INSULIN-DEPENDENT
DIABETES MELLITUS
|
| AE
Karlsen, , P Mose Larsen, SJ Fey, A Nawrocki, MR Larsen,P Roepstorff, HU
Andersen, K
|
| Nielsen,
U Bjerre, T Sparre, F Pociot, T Mandrup-Poulsen, JNerup (Denmark)
|
CHARACTERISATION OF STEM CELL EXPRESSlON USING 2D ELECTROPHORESIS
|
| A.
Pearce, C. Svendsen (Cambridge, UK)
|
TWO-DIMENSIONAL REFERENCE MAP OF HUMAN BRAIN PROTEINS
|
| H.
Langen, P. Berndt, G. Lubec, M. Fountoulakis (Basel,Switzerland; Vienna,
Austria)
|
VISUALIZATION AND CHARACTERISATION OF NEURONAL MEMBRANE PROTEINS
|
| BY
2D GEL ELECTROPHORESIS AND MASS SPECTROMETRY
|
| G.Friso,
L.Wikstöm (Huddinge, Sweden)
|
ACOMPARATIVE PROTEOME ANALYSIS OF HIPPOCAMPAL TISSUE FROM
|
| SCHIZOPHRENIC
AND ALZHEIMER'S DISEASE PATIENTS
|
| P
F Edgar, SKaill, B Dean, R M Faull, R Kydd, G J Cooper (Auckland,
NewZealand; Victoria, Australia)
|
BOVINE HEREDITARY CARDIOMYOPATHY AS A MODEL FOR HUMAN DILATED
|
| CARDIOMYOPATHY:
A PROTEOMIC APPROACH
|
| J.
Weekes, C. H. Wheeler, J. Weil, T. Eschenhagen, G.Scholtysik, M. J. Dunn
|
| (London,
UK; Hamburg, Germany; Bern, Switzerland)
|
PROTEIN SYNTHESIS IN CHONDROCYTIC CELLS EXPOSED TO HYDROSTATIC
|
| PRESSURE
|
| M.
Elo, R. Sironen, K. Kaarniranta, M. B.Goldring , H. J. Helminen, S. Auriola, M.
|
| Lammi
(Kuopio, Finland; Boston, MA, USA)
|
EFFECTS OF IONIZING RADIATION ON MACROPHAGE
|
| K.Koupilová,
L. Hernychová, J. Stulík , A. Macela, W. Kaffenberger, D. van
Beuningen
|
| (Hradec
Králové, Czech Republic; Munich, Germany)
|
A TWO-DIMENSIONAL PROTEIN MAP OF CHINESE HAMSTER OVARY CELLS
|
| K.
Champion, S. Hermes, D. Arnott, S. Weikert, J. Stults, M. Vanderlaan, L.Krummen
|
| (San
Francisco, CA,USA)
|
2-D ELECTROPHORESIS AS A TOOL TO DISCRIMINATE BETWEEN FISH
|
| FILLETS
|
| P.
Roncada, G.F. Greppi (Milan, Italy)
|
USE OF 2D-GEL ELECTROPHORESIS IN ISOLATION AND IDENTIFICATION OF
|
| POLLUTANT
INDUCIBLE BIOMARKER PROTEINS IN FISH
|
| S.Aksnes,
C. Tolfsen, B. Einar Grösvik, A. Goksöyr (Bergen,Norway;
Stavanger, Norway)
|
THE CHARACTERISATION OF HUMAN SPERMATOZOA MEMBRANE PROTEINS
|
| C.
Bohring, W. Krause (Marburg, Germany)
|
IDENTIFICATION OF FUNCTIONAL PROTEINS BY PROTEOME ANALYSIS OF TICK
|
| SALIVARY
GLANDS DURING FEEDING
|
| A.W.H.
Neitz(Pretoria, South Africa)
|
BIOCHEMICAL ANALYSIS OF STAGE SPECIFIC PHAGOSOMAL PROTEINS
|
| M.
Maicher, A. Tiedtke (Münster, Germany)
|
PROTEOMIC ANALYSIS OF ENDOTHELIUM IN VIVO : 2DE MAPPING OF TISSUE-
|
| SPECIFIC
VASCULAR TARGETS
|
| A.
C. Stevens,P. Oh, J. C. Kumer, J. E. Schnitzer (San Diego, CA, USA)
|
TWO-DIMENSIONAL ELECTROPHORETIC SEPARATION AND ANALYSIS OF RET
|
| PROTEIN
IN NIH3T3 TRANSFECTED CELLS AND HUMAN CELL LINES
|
| E.Viganò,
M.Mortarino, I.Bongarzone, A.Negri, M.A.Pierotti, S.Ronchi(Milan, Italy)
|
MAPPING OF HELA CELL PROTEINS TO THE 2D-PAGE (IPG)-SYTEM: SPOT
|
| IDENTIFICATION
BY MALDI-MS AND N-TERMINAL SEQUENCING
|
| A.C.
Shaw, M. Larsen, A. Holm, G. Christiansen, S. Birkelund (Aarhus; Odense;
Copenhagen; Denmark)
|
INFLUENCE OF LOW TEMPERATURE ON PROTEOME AND PROTEIN
|
| PHOSPHORYLATION
OF CHO CELLS
|
| H.
Kaufmann, C. Schwerdel, M. Fussenegger, J. E. Bailey(Zürich,
Swittzerland)
|
AGE-RELATED CHANGES IN THE PROTEIN UNIVERSE IN DIFFERENTIATING
|
| HUMAN
KERATINOCYTES EXAMINED BY 2D-PAGE
|
| S.
Mogensen, B.F.C. Clark, S. I.S. Rattan (Aarhus, Denmark)
|
ANALYSIS OF THE SEROLOGIC RESPONSE TO CANDIDA ALBICANS PROTEINS
BY
|
| 2D-PAGE
|
| A.
Pitarch, M. Pardo, A. Jimenez, J. Pla, M. Sanchez, C.Giland C.Nombela
(Madrid; Salamanca; Spain)
|
ANALYSIS OF SEROLOGIC RESPONSE TO CANDIDA ALBICANS IN A
MURINE
|
| MODEL
|
| R.
Diez-Orejas, M. de la Viuda, M.Pardo., G. Molero, C. Vivar, C. Gil, M. Sanchez,
C. Nombela
|
| (Madrid;
Spain)
|
Posters parallel session : ONCOLOGY
|
GLOBAL EXPRESSION OF ONCOGENE PRODUCTS AND CELL CYCLE SPECIFIC
|
| PROTEINS
IN COLORECTAL EPITHELIAL CELLS
|
| I.
Demalte-Annessi, J.-C. Sanchez, J. Yan, S. Jaccoud, M.A.Reymond, K.
Günther, V. Rouge, D.F.
|
| Hochstrasser
(Geneva, Switzerland; Sydney,Australia; Erlangen, Germany)
|
CHARACTERIZATION OF COLONIC POLYPS BY TWO-DIMENSIONAL GEL
|
| ELECTROPHORESIS
|
| R.
Melis, R. White (Salt Lake City, Utah,USA)
|
MONITORING OF THE EXPRESSION OF S100A9 AND S100A6 CALCIUM-BINDING
|
| PROTEINS
IN HEALTHY AND DISEASED HUMAN COLON MUCOSA
|
| J.
Stulík, K. Koupilová, J.Österreicher,A.Macela, J.
Bureö, P. Jandík, F. Langer, K. Dìdiè
|
| (Hradec
Kralove, Czech Republic)
|
IPG-SDS PAGE PROTEIN MAPS FROM GASTRIC CANCER
|
| S.A.
Tripodi, C. Vindigni, F. Arcuri, A. Carducci, M. Cintorino, L. Bini,Y. Runci,
F. Roviello, D.
|
| Marrelli,
E. Pinto, P. Tosi, V.Pallini (Siena, Italy)
|
2-D PROTEIN PROFILES IN PRIMARY BREAST CANCER-DERIVED CELLS AND
IN
|
| VITRO
SELECTED CLONES
|
| L.
Basirico' L., L. Bini, S.Liberatori, S. Minafra, V. Pallini, I.
Pucci-Minafra (Palermo;Siena; Italy)
|
DIAGNOSIS OF HUMAN OVARIAN TUMORS USING MULTIVARIATE DATA
|
| ANALYSIS
OF PROTEIN EXPRESSION PATTERNS
|
| A.
A. Alaiya, A. Hagman, B. Franzén, B. Moberger,C.Silfversvärd, , S.
Linder, G. Auer
|
| (Stockholm,
Sweden)
|
DEVELOPMENT OF A 2-D PROTEIN DATABASE FOR HUMAN OVARIAN TUMORS
|
| A.
A. Alaiya, A. Hagman, B. Franzén, B. Moberger, C. Silfversvärd,
S.Linder, G. Auer
|
| (Stockholm,
Sweden)
|
TWO-DIMENSIONAL ELECTROPHORESIS OF PROSTATE SPECIFIC ANTIGEN IN
|
| SERA
OF MEN WITH PROSTATE CANCER OR BENIGN PROSTATIC HYPERPLASIA
|
| J.-P.
Charrier, C. Tournel, P. Dalbon, M. Jolivet (Marcy L'Etoile, France)
|
ANALYSIS OF P53 DRIVEN APOPTOTIC EVENTS IN A HUMAN OSTEOSARCOMA
|
| CELL
LINE (SAOS-2) BY 2D GELS AND DNA CHIPS
|
| T.
Voss, K.K.Wilgenbus, W.Spevak (Vienna, Austria)
|
PROTEOMIC IDENTIFICATION OF TUMOR-INDUCED VASCULAR TARGETS
|
| ON
ENDOTHELIUM IN VIVO
|
| A.
C. Stevens, P. Oh, V. Bernardino, J. C. Kumer, M.Caguyong, J. E. Schnitzer
(San Diego, CA, USA)
|
Posters parallel session : SAMPLE PREPARATION AND MASS SPECTROMETRY RELATED TECHNOLOGIES
|
HIGH SENSITIVITY MASS SPECTROMETRIC METHODS FOR OBTAINING INTACT
|
| MOLECULAR
WEIGHTS FROM GEL-SEPARATED PROTEINS
|
| J.
A.Loo, R. R. Ogorzalek Loo (Ann Arbor, MI, USA)
|
MS TOOLS FOR PROTEOMICS: AUTOMATED MS/MS ANALYSIS FOR THE
|
| IDENTIFICATION
OF PROTEINS
|
| C.
A.Settineri, L. M. Nuwaysir, J Mark, B. Sublett (Foster City, CA,USA)
|
CHARACTERIZING PROTEINS BY LC/MS PEPTIDE MAPPING
|
| C.
Settineri , L. Nuwaysir , J. Mark , T. Settineri (Foster City,CA, USA)
|
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