University of Geneva

(Planning of the week September 29^th – October 3^rd )

Week is color coded in to Theory, Practical and theory & Breaks

Day 1 : Monday

10:00 – 11:00 Welcome and Introduction by Dr. Jean-Charles Sanchez and Dr. Odile Carrette ‘Perspective of the week and scope of the course’

11:00 – 12:00 Dr. Odile Carrette ‘SELDI Introduction’

12:00 – 14:00 LUNCH

13:30 – 15:00 SELDI – SAX Profiling – Rententate Chromatography on Chip

15:00 – 16:30 SELDI – ProteinChip Reader Software

16:30 – 17:00 Discussion meeting with trainers

17:00 – 18:00 Dr. Willy Bienvenut ‘Quantitation by chemical modification’

Day 2 : Tuesday

9:00 – 11:00 LC- Prefractionation with SAX spin column

11:00 – 12:00 1DE Tricine Gels

12 :00 – 14 :00 LUNCH

14:00 – 16:00 SELDI - analysis of the fractions

16:00 – 16:30 Discussion meeting with the trainers

16:30 – 17:00 1DE Tricine gels staining

17:00 – 18:00 Dr. Garry Corthals ‘Introduction to Protein identification by MS’

Day 3: Wednesday

9:00 – 10:30 1DE Tricine gel staining and scanning

10:30 – 11:00 1DE - Excision of the Interesting bands for MS

11 :00 – 12 :00 2DE – Dr. Jean-Charles Sanchez ‘2DE sample preparation’

12 :00 – 14 :00 LUNCH

14 :00 – 15 :00 2DE – IPG strip rehydratation

15:00 – 16:30 2DE – Gel Casting

16:30 – 17:00 Discussion meeting with trainers

17:00 -. 18:00 Dr Jean-Charles Sanchez ‘Applications in biomedical proteomics’

Day 4 : Thursday

9:00 – 10:00 2DE – First dimension

10 :00 – 11 :00 2DE – Image analysis software : Melanie 4.0

11 :00 – 12 :00 2DE – Second dimension and staining

12:00 – 14:00 LUNCH

14:00- 16:00 2DE Gel staining

16:00 – 16:30 2DE Gel scanning

16:30 – 17:00 Discussion meeting with trainers

17:00 – 18:00 Dr. Pierre-Alain Binz ‘Bioinformatics and Proteomics’

Day 5 : Friday

9:00 – 11:00 Dr. Sonja Voordijk ‘Image analysis software: MELANIE 4.0’

11:00 – 12:00 Dr. Manfredo Quadroni ‘Sample Preparation for MS’

12:00 – 13:30 LUNCH

13:30 – 15:30 Discussion: Back to your lab, what to do with your own project…